Nr2f6-deficient mice on the C57BL/6 background (Hermann-Kleiter et al., 2008 (link), 2012 (link), 2015 (link)), Nr2f6+/+-OT-II (C57BL/6-Tg(TcraTcrb) 425Cbn/Crl; Nr2f6+/+-OT-II mice) purchased from Charles River and crossed into the Nr2f6-deficient background (Nr2f6−/− -OT-II mice). B6.SJL (CD45.1) on a C57BL/6 background, or B6.SJL crossed to C57BL/6 (CD45.1/CD45.2) were used for adoptive transfer experiments. Mice were maintained under specific pathogen-free conditions (SPF following FELASA guidelines) and used at the age of 6-14 weeks of age (with the exception of the memory experiments). All animal experiments were performed in accordance with national and European guidelines and reviewed and authorized by the committee on animal experiments (BMWFW-66.011/0064-WF/V/3b/2016; BMWFW-66.011/0112-WF/V/3b/2017).
C57bl 6 tg tcratcrb 425cbn crl nr2f6 ot 2 mice
Manufactured by Charles River Laboratories
C57BL/6-Tg(TcraTcrb)425Cbn/Crl; Nr2f6+/+-OT-II mice is a transgenic mouse model that expresses a T cell receptor specific for the ovalbumin peptide 323-339 in the context of I-Ab. This mouse strain can be used for studying T cell responses to ovalbumin.
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2 protocols using c57bl 6 tg tcratcrb 425cbn crl nr2f6 ot 2 mice
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Transcription Factor Nr2f6 Deficient Mice
2
Generation and Characterization of Nr2f6-Deficient Mice
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Nr2f6-deficient mice on the C57BL/6 background (Hermann-Kleiter et al., 2008 (link), Hermann-Kleiter et al., 2012 (link), Hermann-Kleiter et al., 2015 (link)), Nr2f6+/+-OT-II (C57BL/6-Tg(TcraTcrb) 425Cbn/Crl; Nr2f6+/+-OT-II mice) purchased from Charles River and crossed into the Nr2f6-deficient background (Nr2f6−/−-OT-II mice). B6.SJL (CD45.1) on a C57BL/6 background, or B6.SJL crossed to C57BL/6 (CD45.1/CD45.2) were used for adoptive transfer experiments. Mice were maintained under specific pathogen-free conditions (SPF following FELASA guidelines) and used at the age of 6-14 weeks of age (with the exception of the memory experiments). All animal experiments were performed in accordance with national and European guidelines and reviewed and authorized by the committee on animal experiments (BMWFW-66.011/0064-WF/V/3b/2016; BMWFW-66.011/0112-WF/V/3b/2017).
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