Phosphate buffered saline bioperformance certified ph 7.4 pbs

BASF (Badische Anilin- und Soda Fabrik, Ludwigshafen, Germany) with the support of BASF Italia and BTC Chemical Distribution Unit (Cesano Maderno, Monza, and Brianza, Italy) kindly provided Soluplus^® and Solutol^® HS15. Distilled water was obtained from a Simplicity^® UV Water Purification System, Merck Millipore (Merck KGaA, Darmstadt, Germany). Thymoquinone, Phosphate buffered saline BioPerformance Certified pH 7.4 (PBS), Tween^® 80, lecithin (≥99%, TLC) lyophilized powder, cholesterol BioReagent (≥99), Methanol HPLC grade, Acetonitrile HPLC grade, Dimethyl sulfoxide (DMSO) HPLC grade, Formic acid analytical grade, 1,7-octadiene (98%), Dichloromethane (CH2Cl2) were purchased from Sigma–Aldrich (Milan, Italy). Sigma–Aldrich Merck (Milan, Italy) also supplied Dulbecco’s modified Eagle’s medium (DMEM), Ham’s F-12 nutrient mixture, fetal bovine serum (FBS), L-glutamine, penicillin and streptomycin, 1-(4,5-dimethylthiazol-2-yl)-3,5-diphenyl formazan (MTT) and other chemicals. Disposable plastics were obtained from Sarstedt (Nümbrecht, Germany).

Ultrapure water was produced using the Milli-Q-system (Millipore SA, Molsheim, France). Acetonitrile of HPLC and HPLC-MS grades were from Panreac (Barcelona, Spain). Formic acid, hexane, and ethanol of analytical reagent grade were from Sigma-Aldrich (Steinheim, Germany). Standards of tyrosol (>99.5%, Sigma-Aldrich, Steinheim, Germany) and rutin (>99%), luteolin-7-O-glucoside (>98%), verbascoside (>99%), and oleuropein (>98%) from Extrasynthese (Genay, France) were used for quantitative analysis.
Cremophor EL, Tween 80, and Phosphate buffered saline BioPerformance Certified pH 7.4 (PBS), were obtained from Sigma Aldrich (Saint Louis, MO, USA) with the support of Sigma Aldrich Italia (Milan, Italy). Soluplus^® was a gift from BASF (Ludwigshafen, Germany) with the support of BASF Italia, BTC Chemical Distribution Unit (Cesano Maderno, Monza e Brianza, Italy). Captex 300, Captex 355, Labrasol ALF, Capryol 90, Transcutol HP, Labrafac, Labrafilm 1944, Labrafilm 2125, and Lauroglycol 90 were from Gattefossé (Saint Priest, France).