Xevo qtof mass spectrometer q tof

Analyses were performed on an Acquity UPLC (Waters, USA) system coupled to a Xevo QTOF mass spectrometer (Q-TOF, Waters). Separations were performed on a C18 column (Waters Acquity® UPLC C18; 150 mm × 2.1 mm, 1.7 μm). For metabolic fingerprinting, a 2 μL aliquot of the extract was subjected to UPLC analysis using an exploratory gradient with a mobile phase comprising deionized water (A) and acetonitrile (B), both containing formic acid (0.1% v/v). The extracts from melons were subjected to the exploratory gradient as follows: 2%– 95% for 15 min, at a flow rate of 500 μL min^–1. Ionization was performed with an electrospray ionization (ESI) source in negative ion mode, in the range of 110–1200 Da. The optimized instrumental parameters were as follows: capillary voltage of –2800 V, cone voltage of –40 V, source temperature of 120°C, desolvation temperature of 330°C, flow cone gas of 20 L h^–1, desolvation gas flow at 600 L h^–1, and microchannel plate (MCP) detector voltage of –1900 V. The mode of acquisition was MS^E, and the system was controlled using MassLynx 4.1 software (Waters Corporation). The extracts were injected in triplicate.

These analyses were performed using an Acquity UPLC (Waters Corporation, Milford, MA, USA) system coupled to a Xevo qTOF mass spectrometer (Q-TOF, Waters). Separations were performed on a C18 column (Waters Acquity^® UPLC C18; 150 mm × 2.1 mm, 1.7 μm). For metabolic fingerprinting, a 2 μL aliquot of the fraction was subjected to UPLC analysis using an exploratory gradient with a mobile phase comprising deionized water (A) and acetonitrile (B), both containing formic acid 0.1% v/v. The sample was subjected to the exploratory gradient as follows: 2–95% for 15 min, at a flow rate of 500 µL·min⁻¹. Ionization was performed with electrospray ionization (ESI) source in negative and positive ion modes, in the range of 110–1200 Da. The optimized instrumental parameters were as follows: capillary voltage of −2800 V, cone voltage of −40 V, source temperature of 120 °C, desolvation temperature of 330 °C, flow cone gas of 20 L·h⁻¹, desolvation gas flow at 600 L·h⁻¹, and microchannel plate (MCP) detector voltage of −1900 V. The mode of acquisition was MS/MS, and the system was controlled using MassLynx 4.1 software (Waters Corporation, Milford, MA, USA).