Ast kit

Manufactured by Merck Group

Sourced in Sao Tome and Principe, China

The AST kit is a laboratory equipment product designed to perform Antimicrobial Susceptibility Testing (AST). The core function of this kit is to determine the susceptibility of microorganisms to antimicrobial agents, which is crucial for guiding appropriate treatment decisions.

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Lab products found in correlation

Alt kit, by Merck Group (3 mentions) Magnetic rna protein pull down kit, by Thermo Fisher Scientific (1 mentions) Human serum albumin (hsa), by Baxter (1 mentions) Traf6, by Cell Signaling Technology (1 mentions) Real time pcr kit, by Takara Bio (1 mentions) Lipopolysaccharides (lps), by Merck Group (1 mentions) P jnk, by Cell Signaling Technology (1 mentions) Ubiquitin, by Cell Signaling Technology (1 mentions) D galactosamine, by Merck Group (1 mentions) Stat1, by Cell Signaling Technology (1 mentions) Ldh kit, by Abcam (1 mentions)

Spelling variants of this product

AST Activity Assay Kit (34 citations) AST and ALT Activity Assay Kit (7 citations) Aspartate aminotransferase (AST) activity assay kit (5 citations) ALT and AST kits (3 citations) ALT and AST activity assay kits (3 citations)

6 protocols using ast kit

Investigating Inflammatory Liver Injury Mechanisms

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Human serum albumin (HSA) was obtained from Baxter (Deerfield, IL, United States). LPS, D-galactosamine, ALT kit, and AST kit were purchased from Sigma-Aldrich (St Louis, MO, United States). IL-6, IL-22, HMGB1 ELISA kits were purchased from BIKW Co., Ltd. (Beijing, China). Antibodies against Ubiquitin (Cat.3936), TRAF6 (Cat.8028), p38 (Cat.9212), p-p38 (Cat.9216), p65 (Cat.8242), p-p65 (Cat.3033), JNK (Cat.9252), p-JNK (Cat.4668), STAT1 (Cat.14995), and Actin (Cat.3700) were obtained from Cell Signaling Technology (United States). The magnetic RNA-Protein Pull-Down Kit was purchased from Thermo Fisher (United States). Real-time PCR kits were from Takara (Japan). NEAT1 lentivirus, sh-NEAT1 lentivirus, AAV8, and AAV8-NEAT1 were purchased from Genechem (China).

Xu Y., Cao Z., Ding Y., Li Z., Xiang X., Lai R., Sheng Z., Liu Y., Cai W., Hu R., Wang H, & Xie Q. (2019). Long Non-coding RNA NEAT1 Alleviates Acute-on-Chronic Liver Failure Through Blocking TRAF6 Mediated Inflammatory Response. Frontiers in Physiology, 10, 1503.

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Plasma Biomarker Quantification Protocol

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Blood samples were collected and centrifuged for 15 minutes at a speed of 2200–2500 RPM. Plasma was pipetted into separate tubes, CK-MB, AST, and LDH levels were analyzed with CK-MB ELISA kit (Senbeijia, Nanjing, China), AST kit (Sigma-Aldrich, St Louis, USA), and LDH kit (Abcam, Cambridge, UK). The manufacturer’s instructions were followed in all test kits used.

Chen X., Yun C., Zheng H., Chen X., Han Q., Pan H., Wang Y, & Zhong J. (2021). The protective effects of S14G-humanin (HNG) against streptozotocin (STZ)-induced cardiac dysfunction. Bioengineered, 12(1), 5491-5503.

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Serum ALT and AST Quantification

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Serum ALT and AST levels were detected using the ALT kit and AST kit (Sigma Aldrich), according to the manufacturer’s instructions.

Lin Y., Sheng M., Qin H., Zhang P., Wang C., Fu W., Meng X., Wang D, & Hou Y. (2023). Caspase 6 promotes innate immune activation by functional crosstalk between RIPK1-IκBα axis in liver inflammation. Cell Communication and Signaling : CCS, 21, 282.

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Serum ALT and AST Measurement

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Serum samples were diluted, and ALT and AST activity were measured using commercial ALT kit and AST kit (Sigma-Aldrich).

Luo J., Liu H., Xu Y., Yu N., Steiner R.A., Wu X., Si S, & Jin Z.G. (2024). Hepatic Sirt6 activation abrogates acute liver failure. Cell Death & Disease, 15(4), 283.

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AST Inhibition Evaluation Assay

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Inhibition of AST was checked by AST kit (Sigma-Aldrich Co.) according to manufacturer’s instructions. Each compound was checked at a concentration of 100 μg/mL to evaluate its inhibitory potential against AST. Assay was carried out with the positive control provided with the kit. Briefly, in the 96-well flat bottom plate, AST positive control (0.5 μL) and compounds were incubated in AST buffer for 10 minutes at 37°C followed by the addition of AST substrate, AST enzyme mix and AST developer and incubated at 37°C for further 30 minutes. Wells with no compound and containing DMSO were used as a negative control. The plate was read at 450 nm by using Elisa plate reader at the beginning and after every 5 minutes from the commencement of the assay till 30 minutes. For each sample, the inhibition was calculated by subtracting the absorbance at initial time (t=0) from the absorbance at the final time.

Singh S., Khare G., Bahal R.K., Ghosh P.C, & Tyagi A.K. (2018). Identification of Mycobacterium tuberculosis BioA inhibitors by using structure-based virtual screening. Drug Design, Development and Therapy, 12, 1065-1079.

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Plasma AST Quantification Protocol

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Plasma AST activity was detected using an AST kit (Sigma) following manufactory's instructions.

Wang S., Yang Y., Suen A., Zhu J., Williams B., Hu J., Chen F., Kozar R., Shen S., Li Z., Jeyaram A., Jay S.M., Zou L, & Chao W. (2021). Role of extracellular microRNA-146a-5p in host innate immunity and bacterial sepsis. iScience, 24(12), 103441.

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