Cellulose, α- and β-cornstarch, and sucrose were purchased from Oriental Yeast Co., Ltd. (Tokyo, Japan). t-Butylhydroquinone, casein, l -cystine, and soybean oil were from Wako Pure Chemical Industries, Ltd. (Osaka, Japan). The lard, mineral mixture (AIN-93M-Mix), and vitamin mixture (AIN-93-VX) were purchased from MP Biomedicals, LLC (Irvine, CA, USA). All other reagents were of the highest grade available.
Casein
Manufactured by Fujifilm
Sourced in Japan
Casein is a laboratory equipment product manufactured by Fujifilm. It serves as a protein-based reagent commonly used in various analytical and research applications in the life sciences industry. The core function of Casein is to provide a consistent and reliable source of protein for use in experiments and assays, without making claims about its specific intended uses.
Automatically generated - may contain errors
Lab products found in correlation
Sucrose, by Oriental Yeast (4 mentions)
L cystine, by Fujifilm (4 mentions)
T butylhydroquinone, by Fujifilm (4 mentions)
Soybean oil, by Fujifilm (3 mentions)
β cornstarch, by Oriental Yeast (3 mentions)
α cornstarch, by Oriental Yeast (3 mentions)
Sucrose, by Fujifilm (2 mentions)
Boric acid, by Fujifilm (2 mentions)
Glutathione (gsh), by Peptide Institute (1 mentions)
Cf15rxii, by Hitachi (1 mentions)
Trehalose, by Fujifilm (1 mentions)
Peptone, by Fujifilm (1 mentions)
Dri chem immuno ag1 analyzer, by Fujifilm (1 mentions)
Quercetin, by Merck Group (1 mentions)
Choline bitartrate, by Fujifilm (1 mentions)
Bovine serum albumin (bsa), by Merck Group (1 mentions)
Sodium hydroxide (naoh), by Fujifilm (1 mentions)
Sucrose octasulfate sodium salt, by LGC (1 mentions)
Trichloroacetic acid, by Fujifilm (1 mentions)
Pepsin, by Fujifilm (1 mentions)
12 protocols using casein
1
Comparison of Dietary Carbohydrate Sources
2
Recombinant proCCN Production in E. coli
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Glutathione and Bz-Arg-OEt were purchased from the Peptide Institute, Inc. (Osaka, Japan). Casein was purchased from Fuji Film Wako Pure Chemicals, Ltd. (Osaka, Japan). All chemicals and solvents used were of reagent grade. The cDNA encoding proCCN (Supplementary Materials Figure S4 ) was prepared by Eurofins Japan (Tokyo, Japan), and its codon bias was optimized for an E. coli expression system using the manufacture’s program GENEius (http://www.geneius.de/ ; accessed on 13 May 2017). Protein solutions and E. coli cells were centrifuged using CF15RXII (HITACHI, Tokyo, Japan).
3
Crickets and Cellulose-Based Diet
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Cellulose powder used in the present study was purchased from Nacalai tesk (Kyoto, Japan). Dextrin hydrate and casein were purchased from Wako Pure Chemical Industries (Osaka, Japan). For cricket food, rabbit ORC4 was purchased from ORIENTAL YEAST Co., Ltd. (Tokyo, Japan) and a commercially available diet of cat food (Friskies dry mix) was purchased from Nestle Purina PetCare Co., Ltd. (Hyogo, Japan).
4
Dietary Supplement Composition Analysis
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EPA (95.0% purity) was purchased from Combi-blocks, Inc. (San Diego, CA, USA). α-LA (>98.0% purity), casein, soybean oil, l -cystine, and t-butylhydroquinone were purchased from Wako Pure Chemical Industries (Osaka, Japan). Cellulose, α-cornstarch, β-cornstarch, and sucrose were obtained from Oriental Yeast Co., Ltd. (Tokyo, Japan). Vitamin mix (AIN-93-VX) and mineral mix (AIN-93G-Mix) were from MP Biomedicals, LLC (Irvine, CA, USA). All other reagents were of the highest grade available.
5
Drosophila Rearing Conditions Protocol
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Drosophila melanogaster flies were reared on standard agar-cornmeal media at 25 °C unless otherwise indicated8 (link), 50 (link). The detailed food compositions, except preservatives, are shown in Fig.4B . Casein, peptone, and trehalose were obtained from Wako Chemical, BD Biosciences, and Hayashibara, Co., respectively. All experiments were conducted under non-crowded conditions. No yeast paste was added to the fly tubes for any of the experiments. The percentage of puparium formation and adult flies was determined by counting homozygotes and heterozygotes in the same vials as an internal control.
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Drosophila melanogaster flies were reared on standard agar-cornmeal media at 25 °C unless otherwise indicated8 (link), 50 (link). The detailed food compositions, except preservatives, are shown in Fig.
6
Plasma Biomarker Detection Protocol
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Plasma from African and European healthy donors were 5-fold diluted with an extraction buffer (Tris EDTA buffer pH7.7 containing 0.1% Casein (Wako, Japan), 1% BIGCHAP (Dojindo, Japan)). 5-fold diluted plasma (140 μl final volume) was applied to the sample application point of the diagnostic cartridge. Then, the cartridge was inserted into FUJI DRI-CHEM IMMUNO AG1 analyzer (FUJIFILM, Japan), in which silver amplification was performed automatically. The results were interpreted based on the displayed information and the measured delta OD.
7
Quercetin Supplementation in Animal Diets
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Quercetin (≥95%) was purchased from Sigma-Aldrich (St. Louis, MO, USA). Cellulose, α-cornstarch, β-cornstarch, and sucrose were purchased from Oriental Yeast Co., Ltd. (Tokyo, Japan). Casein, soybean oil, L-cystine, and t-butylhydroquinone were purchased from Wako Pure Chemical Industries, Ltd. (Osaka, Japan). The vitamin mixture (AIN-93-VX), mineral mixture (AIN-93G-Mix), and lard were purchased from MP Biomedicals, LLC (Irvine, CA, USA). All reagents used were of the highest grade available.
8
Dietary Lipids and Nutrient Analysis
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Soybean oil and fish oil were obtained from J-Oil Mills, Inc. (Yokohama, Japan). Lard, vitamin mixture (AIN-93-VX), and mineral mixture (AIN-93G-Mix) were obtained from MP Biomedicals, LLC (Irvine, CA, USA). Cellulose, α-cornstarch, β-cornstarch, and sucrose were purchased from Oriental Yeast Co., Ltd. (Tokyo, Japan). Casein, L-cystine, choline bitartrate, and t-butylhydroquinone were obtained from Wako Pure Chemical Industries, Ltd. (Osaka, Japan). All other reagents were of the highest grade available.
9
Sensitive Aflatoxin B1 Immunoassay
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Analytical-grade reagents and
18 MΩ·cm resistance water (obtained from a Barnstrad Milli-Q
purification system) were used throughout the experiments. Nitrocellulose
membrane (Immunopore RP, code number 78356403) was purchased from
GE Healthcare Japan Ltd. Absorbent pads (CFSP203000) were purchased
from the EMD Millipore Corporation. The BCIP/NBT (5-bromo-4-chloro-39-indolyphosphate p-toluidine salt, nitro-blue tetrazolium chloride) substrate
solution, substrate buffer solution, and methanol were purchased from
Nacalai Tesque (Kyoto, Japan). An alkaline phosphatase-labeling kit
was purchased from Dojindo (Rockford, IL, U.S.A.). Aflatoxin B1–BSA conjugate from Aspergillus flavus (AFB1–BSA), aflatoxin B1 antigen (AFB1), and bovine serum albumin (BSA) were purchased from Sigma-Aldrich
(St. Louis, MO, U.S.A.). Anti-aflatoxin B1 antibody and
goat anti-mouse IgG H&L were purchased from Abcam (Cambridge,
U.K.). Boric acid, casein, sucrose, polyoxyethylene (20), sorbitan
monolaurate (Tween 20), tris(hydroxymethyl)aminomethane, and sodium
hydroxide were purchased from Wako (Wako Pure Chemical Industries,
Japan).
18 MΩ·cm resistance water (obtained from a Barnstrad Milli-Q
purification system) were used throughout the experiments. Nitrocellulose
membrane (Immunopore RP, code number 78356403) was purchased from
GE Healthcare Japan Ltd. Absorbent pads (CFSP203000) were purchased
from the EMD Millipore Corporation. The BCIP/NBT (5-bromo-4-chloro-39-indolyphosphate p-toluidine salt, nitro-blue tetrazolium chloride) substrate
solution, substrate buffer solution, and methanol were purchased from
Nacalai Tesque (Kyoto, Japan). An alkaline phosphatase-labeling kit
was purchased from Dojindo (Rockford, IL, U.S.A.). Aflatoxin B1–BSA conjugate from Aspergillus flavus (AFB1–BSA), aflatoxin B1 antigen (AFB1), and bovine serum albumin (BSA) were purchased from Sigma-Aldrich
(St. Louis, MO, U.S.A.). Anti-aflatoxin B1 antibody and
goat anti-mouse IgG H&L were purchased from Abcam (Cambridge,
U.K.). Boric acid, casein, sucrose, polyoxyethylene (20), sorbitan
monolaurate (Tween 20), tris(hydroxymethyl)aminomethane, and sodium
hydroxide were purchased from Wako (Wako Pure Chemical Industries,
Japan).
10
Sucrose Octasulfate Oral Administration
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Sucrose octasulfate sodium salt (Toronto Research Chemicals, Inc., Toronto, ON, Canada) was dissolved in distilled water for all experiments and was administered orally at a volume of 2.5 mL/kg of body weight. The following drugs and reagents were also utilized: isoflurane inhalation solution (Pfizer, New York, NY, USA); 10% sucralfate-containing suspension (ULCERLMIN® Oral Suspension 10%; Chugai Pharmaceutical Co. Ltd., Tokyo, Japan); pepsin (1:10,000, from porcine stomach mucosa), casein, trichloroacetic acid, sodium carbonate, and Folin’s reagent (all purchased from Wako Pure Chemical Industries, Ltd., Osaka, Japan); and 9-aminoacridine (9-AA; Thermo Fisher Scientific K.K., Yokohama, Japan).
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