α sarcomeric actin

Manufactured by Abcam

Sourced in United States, United Kingdom

α-sarcomeric actin is a cytoskeletal protein that plays a crucial role in muscle contraction. It is a major component of the thin filaments within the sarcomeres, the basic unit of skeletal and cardiac muscle. This protein is essential for the structural integrity and function of muscle tissues.

Automatically generated - may contain errors

Lab products found in correlation

Spelling variants of this product

α-actin (39 citations) Mouse monoclonal α-sarcomeric actin antibody (2 citations)

7 protocols using α sarcomeric actin

Immunoblotting Techniques for Cellular Protein Analysis

Check if the same lab product or an alternative is used in the 5 most similar protocols

Immunoblotting was performed as previously described.²⁵ Specific antibodies employed are as follows: LC3 (Novus Biologicals, NB100-2220); p62 (Abcam, ab56416); TRAF2 (Abcam, ab126758); COX IV (Abcam, ab14744); TOMM20 (Sigma, WH0009804M1); VDAC (Cell Signaling Technology, 4661S); PARKIN (Abcam, ab15954); FACL4 (Abcam, ab155282); calreticulin Antibody #2891; VAPB (Thermo Fisher Scientific, A302-894A); IRE1α (14C10) (Cell Signaling Technology, 3294S); GAPDH (Abcam, ab22555); TLR9 (Novus Biologicals, NBP2-24729); actin (Sigma, A2066); PINK1 (MRC PPU products and reagents, S774C [DU17570] and S086D [DU34559]); and α-sarcomeric actin (Abcam, ab52219).

Ma X., Rawnsley D.R., Kovacs A., Islam M., Murphy J.T., Zhao C., Kumari M., Foroughi L., Liu H., Qi K., Diwan A., Hyrc K., Evans S., Satoh T., French B.A., Margulies K.B., Javaheri A., Razani B., Mann D.L., Mani K, & Diwan A. (2021). TRAF2, an Innate Immune Sensor, Reciprocally Regulates Mitophagy and Inflammation to Maintain Cardiac Myocyte Homeostasis. JACC: Basic to Translational Science, 7(3), 223-243.

+ Open protocol

+ Expand

Immunofluorescence Staining of Cardiac Cells

Check if the same lab product or an alternative is used in the 5 most similar protocols

Immunofluorescence staining was performed for the detection of cardiomyocytes (α sarcomeric actin, and Dianova), macrophages (F4/80, abcam, Waltham, MA, USA), and T-lymphocytes (CD3, abcam) on paraffin-embedded LV heart tissues of the transverse cross-sections (3 µm). After heat-mediated antigen retrieval with citraconic anhydride solution, incubation with the first antibody was performed by overnight incubation at 4 °C, additional at 37 °C for 1 h, and subsequently with the appropriate secondary antibody at 37 °C for 1 h. In 15 areas at 100× magnification, positive cells were counted against all of the DAPI-positive cardiomyocytes.

Bettink S.I., Reil J.C., Kazakov A., Körbel C., Millenaar D., Laufs U., Scheller B., Böhm M, & Schirmer S.H. (2022). Inhibition of TRIF-Dependent Inflammation Decelerates Afterload-Induced Myocardial Remodeling. Biomedicines, 10(10), 2636.

+ Open protocol

+ Expand

Protein Extraction and Analysis from Myocardial Tissue

Check if the same lab product or an alternative is used in the 5 most similar protocols

For protein extraction from myocardial tissue block, tissue block was weighed and treated with ice-cold lysis buffer (60 µL per mg of tissue) with protease inhibitor, followed by being homogenized with the homogenizer at low speed (30 sec each cycle with a 1-min break on ice) until complete lysis. The protein lysate was centrifuged at 12,000 rpm at 4 ℃ for 20 min, and the supernatant was collected and stored at −80 ℃ for further use.
BCA Protein Assay Kit was used in determining Protein concentration (Thermo Scientific, USA). The protein extract underwent electrophoresis using 10% SDS/PAGE gels and later transferred onto PVDF membrane (Merck Millipore, Germany). Antibodies including vimentin (Santa Cruz, USA), cardiac troponin T (cTnT, Santa Cruz, USA), connexin 43 (Cell Signaling, USA), c-Kit (Santa Cruz, USA), CD31 (Santa Cruz, USA), α-smooth muscle actin (α-SMA, Abcam, USA), α-sarcomeric actin (Abcam, USA), rabbit anti-p38α (Santa Cruz, USA), mouse anti p-p38 (Santa Cruz, USA), GAPDH (Proteintech, USA) were all utilized for Western blot analyses. Western blot bands were quantitated by Quantity One software (BioRad, USA).

Duan X., Yang J., Zhang W., Yang X., Xia Y., Gan S., Kuang S., Cheng X., Xie S, & Liu Y. (2023). Isoproterenol pre-treatment improve the therapeutic efficacy of cardiosphere-derived cells transplantation for myocardial infarction. Journal of Thoracic Disease, 15(6), 3089-3105.

+ Open protocol

+ Expand

Cardiomyocyte Immunofluorescence Staining

Check if the same lab product or an alternative is used in the 5 most similar protocols

After indicated treatment, cardiomyocytes were rinsed with PBS for 3 times and fixed with 4% paraformaldehyde for 20 min. Then, cells were rinsed with PBS and permeabilized with 0.1% Triton X-100 (Merck, Darmstadt, GER) for 5 min; After rinsed with PBS, cells were then stained with α-sarcomeric actin (abcam, Cambridge, UK) followed by a fluorescent secondary antibody (abcam, Cambridge, UK). Cells were visualized under a Leica fluorescence microscope and cell sizes were measured by NIH Image J (1.51e) software.

Han X., Wang Y., Fu M., Song Y., Wang J., Cui X., Fan Y., Cao J., Luo J., Sun A., Zou Y., Hu K., Zhou J, & Ge J. (2019). Effects of Adiponectin on Diastolic Function in Mice Underwent Transverse Aorta Constriction. Journal of Cardiovascular Translational Research, 13(2), 225-237.

+ Open protocol

+ Expand

Immunofluorescent Staining of Notch1 ICD and α-Sarcomeric Actin

Check if the same lab product or an alternative is used in the 5 most similar protocols

Immunofluorescent staining was performed as described in our previous publication [12 (link), 23 (link)]. Briefly, the paraffin-embedded heart sections (3 μm) were incubated in 1% normal donkey serum in PBS containing 0.3% Triton X-100 (1 h, 37°C). The primary antibodies to Notch1 ICD and α-sarcomeric actin (Abcam, Cambridge, UK, 1 : 50 dilution) were added and incubated with the sections overnight (4°C). Then, the paraffin-embedded heart sections were washed with PBS 3 times and incubated with Cy3-conjugated goat anti-rabbit IgG and FITC-conjugated donkey anti-goat IgG (Abbkine, Redlands, CA, USA). 4′,6-Diamidino-2-phenylindole (DAPI, Sigma-Aldrich, MO, USA) was used to stain the nuclei. The cellular distribution of Notch1 ICD and α-sarcomeric actin was observed using a confocal microscope (FV1000, Olympus, Tokyo, Japan). Five fields of each section were randomly chosen and photographed. The graphs were analyzed and calculated using Image-Pro Plus software (Media Cybernetics, MA, USA).

Yu L., Li Z., Dong X., Xue X., Liu Y., Xu S., Zhang J., Han J., Yang Y, & Wang H. (2018). Polydatin Protects Diabetic Heart against Ischemia-Reperfusion Injury via Notch1/Hes1-Mediated Activation of Pten/Akt Signaling. Oxidative Medicine and Cellular Longevity, 2018, 2750695.

+ Open protocol

+ Expand

Cardiomyocyte Differentiation Protocol

Check if the same lab product or an alternative is used in the 5 most similar protocols

Cardiomyocyte differentiation was induced using cardiomyocyte differentiation medium [CDM; 2% FBS ESQ (embryonic stem cell qualified) (Invitrogen), 1% insulin transferring selenium in IMDM: DMEM/F12; 1:1 (Sigma-Aldrich)] supplemented with 1 mM DMSO. The DMSO-supplemented CDM was changed every 2 days for 6 days. Cells were washed with PBS (Invitrogen) to remove the dead cells, and 2 ml CDM supplemented with 0.1 mM ascorbic acid (Sigma-Aldrich) was added to the plate. The medium was changed every 2 days for the following 6 days (hereafter, the cells treated under such conditions are labeled as “DMSO-treated cells” for simplicity). After that, the cells were fixed with paraformaldehyde (Sigma-Aldrich) and immunostained with antibodies against α-sarcomeric actin (1:1,000; Abcam), cardiac troponin T (1:1,000; Abcam), or lysed for harvesting of protein and RNA. Negative control cells were treated with CEM for 12 days, with the medium changed every 2 days.

Tan S.C., Gomes R.S., Yeoh K.K., Perbellini F., Malandraki-Miller S., Ambrose L., Heather L.C., Faggian G., Schofield C.J., Davies K.E., Clarke K, & Carr C.A. (2015). Preconditioning of Cardiosphere-Derived Cells With Hypoxia or Prolyl-4-Hydroxylase Inhibitors Increases Stemness and Decreases Reliance on Oxidative Metabolism. Cell transplantation, 25(1), 35-53.

+ Open protocol

+ Expand

Histological Analysis of Mouse Heart Post-TAC

Check if the same lab product or an alternative is used in the 5 most similar protocols

Mouse heart tissues were collected 4 weeks after TAC. The heart was fixed with paraformaldehyde, embedded in paraffin and cut into slices of 4 μm thickness for histological analysis. The size and morphological alterations of the heart tissue were assessed by hematoxylin/eosin (HE) and Masson trichrome staining. Paraffin-embedded tissue sections were stained with mouse monoclonal α-sarcomeric actin (α-actin) antibody (1:75, Abcam) and Alexa Fluor 594 goat anti-mouse antibody (1:200, Abcam) as secondary antibody. Cell nucleus was stained with DAPI (Sigma-Aldrich, Merck KGaA). Sections were mounted and analyzed under a confocal microscope (Zeiss, GmbH). The heart tissues collected from six mice in each group were analyzed [20 (link)].

Ding Y., Wang J, & Lu J. (2021). miR-337-5p promotes the development of cardiac hypertrophy by targeting Ubiquilin-1 (UBQLN1). Bioengineered, 12(1), 6771-6781.

+ Open protocol

+ Expand

About PubCompare

Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.

We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.

However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.

Ready to get started?

Revolutionizing how scientists
search and build protocols!