Methyl p-toluenesulfonate, ethylenediamine, and super dehydrated acetonitrile were purchased from Wako Pure Chemical Industries, Ltd. (Osaka, Japan). 2-Ethyl-2-oxazoline was purchased from Tokyo Chemical Industry Co., Ltd. (Tokyo, Japan). 1-Ethyl-3-(3-dimethylaminopropyl) carbodiimide hydrochloride (WSC) was purchased from Dojindo Molecular Technologies, Inc. (Kumamoto, Japan). 4-Dimethylaminopyridine (DMAP) and 2 mol/l-hydrochloric acid were purchased from Nacalai Tesque, Inc. (Kyoto, Japan). Poly(2-Ethyl-2-oxazoline) (molecular weight: 25, 50, and 200 kDa) was purchased from Sigma-Aldrich Japan (Tokyo, Japan). 1H-Benz[e]indolium, 2-[7-[3-(5-carboxypentyl)-1,3-dihydro-1,1-dimethyl-2H-benz[e]indol-2-ylidene]-1,3,5-heptatrien-1-yl]-1,1-dimethyl-3-(4-sulfobutyl), inner salt (ICCA) was synthesized according to previously reported method32 (link). All other chemicals of highest purity available were used.
Ethylenediamine
Manufactured by Fujifilm
Sourced in Japan
Ethylenediamine is a colorless, volatile liquid chemical compound. It is used as a building block in the synthesis of various organic compounds and as a hardening agent in epoxy resins.
Automatically generated - may contain errors
Lab products found in correlation
Super dehydrated acetonitrile, by Fujifilm (2 mentions)
2 ethyl 2 oxazoline, by Tokyo Chemical Industry (2 mentions)
Methyl p toluenesulfonate, by Fujifilm (2 mentions)
4 dimethylaminopyridine dmap, by Nacalai Tesque (1 mentions)
Poly 2 ethyl 2 oxazoline, by Merck Group (1 mentions)
Milli q system, by Merck Group (1 mentions)
Oral swab, by Salimetrics (1 mentions)
Sia kit, by GE Healthcare (1 mentions)
N ethyl n 3 dimethylaminopropyl carbodiimide hydrochloride, by GE Healthcare (1 mentions)
N hydroxysuccinimide nhs, by GE Healthcare (1 mentions)
4 4 azobis 4 cyanovaleric acid v 501, by Fujifilm (1 mentions)
Dpaema, by Merck Group (1 mentions)
Tris 2 carboxyethyl phosphine hydrochloride tcep, by Fujifilm (1 mentions)
2 isopropyl 2 oxazoline, by Tokyo Chemical Industry (1 mentions)
S 2 4 isothiocyanatobenzyl 1 4 7 10 tetraazacyclododecane tetraacetic acid p scn bn dota, by Macrocyclics (1 mentions)
1 2 dioleoyl sn glycero 3 phosphoethanolamine, by NOF corporation (1 mentions)
Fluorescein isothiocyanate (fitc), by Dojindo Laboratories (1 mentions)
Phospholipid c test wako, by Fujifilm (1 mentions)
Dulbecco s phosphate buffered saline pbs, by Nissui Pharmaceutical (1 mentions)
5 protocols using ethylenediamine
1
Synthesis of Fluorescent Poly(2-Ethyl-2-Oxazoline)
2
Surface Plasmon Resonance Cortisol Assay
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Mouse anti-cortisol monoclonal antibody (association constant KA = 1.7 × 109 M−1, cortisol antibody) was purchased from Abcam PLC (UK). As the cortisol analog, hydrocortisone 3-(O-carboxymethyl) oxime (hydrocortisone 3-CMO) was purchased from Sigma Aldrich (USA). PEG6-COOH aromatic dialkanethiol (PEG6-COOH) was purchased from SensoPath Technologies, Inc. (USA), and was used as a reagent to form SAMs on the chip surface. The Au sensor chip included in the SIA Kit, the N-ethyl-N-(3-dimethylaminopropyl) carbodiimide hydrochloride (EDC) included in an amine coupling kit, the N-hydroxysuccinimide (NHS) for sensor surface fabrication, a borate buffer (pH 8.5, 10 mM disodium tetraborate, 1 M NaCl), and 50 mM NaOH were purchased from GE Healthcare Bio-Science AB (Sweden). Ethylenediamine was purchased from Wako Pure Chemical Industries, Ltd. (Japan). An ELISA kit was used as a conventional salivary cortisol detection method, and Salimetrics oral swabs, used as a salivary collection material were purchased from Salimetrics LLC (USA). Water purified using a Milli-Q integral water purification system was used as the solvent. All aqueous solutions were prepared with Milli-Q water obtained from a Milli-Q system (Millipore Corporation, USA).
3
Synthesis and Purification of Polymeric Fluorescent Probes
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DPAEMA (97%) was obtained from Sigma-Aldrich (St. Louis, MO, USA) and purified using a Sigma-Aldrich inhibitor removal column. MPC was purchased from NOF Corp. (Tokyo, Japan), which was synthesized and purified following a previously reported method [28 (link)]. 4-Cyanopentanoic acid dithiobenzoate (CPD) was synthesized as a chain transfer agent (CTA) according to a reported procedure [29 (link)]. Tris(2-carboxyethyl)phosphine hydrochloride (TCEP, 98%), ethylenediamine (>99.0%), and 4,4′-azobis-(4-cyanovaleric acid) (V-501, 98%) were purchased from Wako Pure Chemical (Osaka, Japan) and used without any purification procedure. Pyrene (Py, 97%) was supplied by Wako Pure Chemical and purified via recrystallization from methanol. Alexa Fluor 555 C2 maleimide (A555) and Alexa Fluor 488 C5 maleimide (A488) were purchased from Thermo Fisher Scientific (Tokyo, Japan) and used as received. Methanol and ethanol were dried over 4Å molecular sieves and purified via distillation. Water was purified using an ion-exchange column.
4
Radiopharmaceutical Synthesis Protocols
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Methyl p-toluenesulfonate, ethylenediamine, and super-dehydrated acetonitrile were purchased from Wako Pure Chemical Industries, Ltd. 2-ethyl-2-oxazoline, 2-isopropyl-2-oxazoline, and 2-propyl-2oxazoline were purchased from Tokyo Chemical Industry Co., Ltd. S-2-(4-isothiocyanatobenzyl)-1,4,7,10-tetraazacyclododecane tetraacetic acid (p-SCN-Bn-DOTA) was purchased from Macrocyclics Inc. 111 In chloride ( 111 InCl 3 ) was kindly supplied by Nihon Medi-Physics. 90 Y-chloride ( 90 YCl 3 ) was purchased from Eckert & Zieglr Radiopharma GmbH. Other reagents were of reagent grade and were used without further purification unless otherwise indicated.
5
Synthesis and Characterization of Lipid-PEG Conjugates
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Boc-Protected-amino-PEG-carbonate-NHS (Boc-PEG-NHS, Mw 5000), methoxy-PEG-carbonate-NHS (MeO-PEG-NHS, Mw 5000), 1,2-dimyristoyl-sn-glycero-3-phosphoethanolamine (DMPE), 1,2dipalmitoyl-sn-glycero-3-phosphoethanolamine (DPPE), 1,2-distearoyl-sn-glycero-3-phosphoethanolamine (DSPE), and 1,2-dioleoyl-sn-glycero-3-phosphoethanolamine (DOPE) were purchased from NOF Corporation (Tokyo, Japan). 1-Tetradecanoyl-sn-glycero-3-phosphoethanolamine (lysoPE (m=12)), 1hexadecanoyl-sn-glycero-3-phosphoethanolamine (lysoPE (m=14)), and 1-octadecanoyl-sn-glycero-3phosphoethanolamine (lysoPE (m=16)) were purchased from Avanti Polar Lipids, Inc. (Alabaster, AL).
Fluorescein isothiocyanate (FITC) was purchased from Dojindo Laboratories (Kumamoto, Japan). Dichloromethane, chloroform, diethyl ether, toluene, N,N'-dimethylformamide (DMF), ethanol, triethylamine (TEA), and tetrahydrofuran (THF) were purchased from Nacalai Tesuque (Kyoto, Japan). 1,6-Diphenyl-1,3,5-hexatriene (DPH), trifluoroacetic acid (TFA), ethylenediamine, phospholipid C-test wako were purchased from Wako Pure Chemical Industries, Ltd. (Osaka, Japan). Dulbecco's phosphate buffered saline (PBS) was purchased from Nissui Pharmaceutical, Co., Ltd. (Tokyo, Japan). Cholesteryl chloroformate was obtained from Tokyo Chemical Industry Co., Ltd. (Tokyo, Japan). All of these reagents were used as obtained.
Fluorescein isothiocyanate (FITC) was purchased from Dojindo Laboratories (Kumamoto, Japan). Dichloromethane, chloroform, diethyl ether, toluene, N,N'-dimethylformamide (DMF), ethanol, triethylamine (TEA), and tetrahydrofuran (THF) were purchased from Nacalai Tesuque (Kyoto, Japan). 1,6-Diphenyl-1,3,5-hexatriene (DPH), trifluoroacetic acid (TFA), ethylenediamine, phospholipid C-test wako were purchased from Wako Pure Chemical Industries, Ltd. (Osaka, Japan). Dulbecco's phosphate buffered saline (PBS) was purchased from Nissui Pharmaceutical, Co., Ltd. (Tokyo, Japan). Cholesteryl chloroformate was obtained from Tokyo Chemical Industry Co., Ltd. (Tokyo, Japan). All of these reagents were used as obtained.
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