Blebbistatin (B05060), low-endotoxin BSA (A8806), PA (P9767), and Y27632 (Y0503) were from Sigma Aldrich (St. Louis, MO), while cis-PO/palmitoleic acid (10009871) was from Cayman Chemicals (Ann Arbor, MI). Antibodies used for immunoblotting were α-actinin (Millipore Sigma; A7811), β-actin (Cell Signaling; 4790), α-catenin (ThermoFisher; 13-9700), β-catenin (BD Biosciences; 610153), VE-cadherin (Santa Cruz; 9989), RhoA (Santa Cruz; c-418), IRDye 800CW goat anti-rabbit immunoglobulin G (IgG) (LI-COR; 9253211), and IRDye 680LT goat anti-mouse IgG (92668070). Antibodies and reagents used for immunofluorescence were α-catenin (ThermoFisher; 13-9700), β-catenin (BD Biosciences; 610153), DAPI (4’,6-Diamidino-2-phenylindole, dihydrochloride; ThermoFisher; D1306), GM130 (BD Biosciences; 610823), phospho-MLC (Cell Signaling; 3674), phalloidin (Alexa Fluor 555) (ThermoFisher; A34055), phospho-YAP S127 (Abcam; 76252), YAP (Cell Signaling 14074), VE-cadherin (Santa Cruz; 9989), goat anti-rabbit IgG secondary antibody (Alexa Fluor 488) (ThermoFisher; A11008), goat anti-mouse IgG secondary antibody (Alexa Fluor 647) (ThermoFisher; A21235). Dako fluorescence mounting medium (S3023) was from Aligent. Y227632 (Y0503) was from Millipore Sigma (Burlington, MA). The plasmid encoding GFP–β-actin was kindly provided by Sergio Grinstein (Hospital for Sick Children, Toronto, Ontario, Canada).
Pa p9767
Manufactured by Merck Group
Sourced in United States
The PA (P9767) is a laboratory equipment product manufactured by Merck Group. It serves as a core function for various laboratory applications. The detailed technical specifications and intended use of this product are not included in this factual and unbiased description.
Automatically generated - may contain errors
Lab products found in correlation
4 6 diamidino 2 phenylindole (dapi), by Thermo Fisher Scientific (2 mentions)
Irdye 680lt goat anti mouse igg, by LI COR (1 mentions)
Ve cadherin, by Santa Cruz Biotechnology (1 mentions)
Phospho mlc, by Cell Signaling Technology (1 mentions)
Blebbistatin, by Merck Group (1 mentions)
Low endotoxin bsa, by Merck Group (1 mentions)
α catenin, by Thermo Fisher Scientific (1 mentions)
Irdye 800cw goat anti rabbit immunoglobulin g igg, by LI COR (1 mentions)
Phospho yap s127, by Abcam (1 mentions)
Alexa fluor 647 goat anti mouse igg secondary antibody, by Thermo Fisher Scientific (1 mentions)
Y 27632, by Merck Group (1 mentions)
β actin, by Cell Signaling Technology (1 mentions)
α actinin, by Merck Group (1 mentions)
β catenin, by BD (1 mentions)
Gm130, by BD (1 mentions)
3 ma m9281, by Merck Group (1 mentions)
2 protocols using pa p9767
1
Immunoblotting and Immunofluorescence Assay Protocol
2
Autophagy Regulation in Reproductive Physiology
Check if the same lab product or an alternative is used in the 5 most similar protocols
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CQ (100421-200401) was purchased from the National Institutes for Food and Drug Control (Beijing, China), and RAP (R-5000) was purchased from LC Laboratories (Woburn, MA, USA). 3-MA (M9281) was purchased from Sigma (St. Louis, MO, USA). The rabbit anti-SQSTM1/p62 monoclonal antibody (ab109012) for western blot was purchased from Abcam (Cambridge, UK). The rabbit anti-phospho-mTOR antibody (#2974), rabbit anti-mTOR antibody (#2983), rabbit anti-BECLIN1 antibody (#3495) and rabbit anti-LC3II/I antibody (#12741) for western blot were purchased from Cell Signaling Technology (Beverly, MA, USA). The rabbit anti-GAPDH antibody (sc-25778) for western blot was purchased from Santa Cruz Biotechnology (Dallas, TX, USA). The secondary antibody was purchased from LI-COR Biosciences. Oestradiol (E2, E-EL-0065c), testosterone (T, E-EL-0072c), follicle stimulating hormone (FSH, E-EL-M0511c), and luteinizing hormone (LH, E-EL-M0057c) detection kits were purchased from Elabscience Biotechnology Co., Ltd. (Wuhan, China). PA (P9767) was purchased from Sigma (St. Louis, MO, USA). All the other chemicals used in our study were of analytical grade.
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