For direct qualitative analysis, 100 mg of lyophilized plant material was extracted with 5 mL of MeOH: H2O (8:2 v/v) in an ultrasonic bath for 20 min at 25 °C. The supernatant was collected and evaporated to dryness. Extracts were suspended in 2 mL of 0.2% formic acid (FA) in H2O prior to preliminary purification and concentration using solid phase extraction (SPE). SPE was performed using an Oasis Max 1cc Vac Cartridge, 500 mg Sorbent per Cartridge, 30 µm Particle Size (Waters Corporation, Milford, MA, USA) conditioned with MeOH (4 mL) and H2O (4 mL). The sample was loaded and cartridges washed with 5 mL water in 0.1% FA. The phenolic compounds were eluted with 5 mL of 80% MeOH in 0.1% FA. Samples were re-evaporated to dryness and dissolved in 25% of MeOH to obtain 1 mg dry weight/mL baseline plant material concentration.
Mg sorbent per cartridge
Manufactured by Waters Corporation
Sourced in United States
The Mg Sorbent per Cartridge is a laboratory equipment product designed for sample preparation and extraction. It serves as a sorbent material within a cartridge format, providing a means to selectively isolate and concentrate analytes of interest prior to further analysis. The product's core function is to facilitate efficient sample cleanup and analyte enrichment, enabling more accurate and reliable analytical results. No further details or interpretations are provided.
Automatically generated - may contain errors
Lab products found in correlation
2 protocols using mg sorbent per cartridge
1
Phenolic Compounds Extraction from Plants
2
Muscle Bioactive Extraction Protocol
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
To extract the BA, 100 g of muscle was blended with 40 mL of acetonitrile and 20 mL of isopropanol during 30 s. NaCl (1.2 g) was added to the blend, mixed on vortex for 2 min, supplemented with 4 g of Na 2 SO 4 and 0.5 g of MgSO 4 and mixed again for 2 min (Water, 2012) . Lastly, the samples were centrifuged (Hermle Model Z323K, Germany) at 1960 g for 10 min. The supernatant was dried using a rotary evaporator (Büchi R-215, Labortechnik, Switzerland). The pellet was dissolved in 1 mL of 0.1% formic acid, sonicated for 2 min, passed through cartridges (Oasis MCX 3 cc Vac Cartridge, 60 mg Sorbent per cartridge, 30 µm, Waters, USA) and filtered (Membrane Filter, 0.45 µm and 0.22 µm pore size, Merck Millipore, Ireland) (Water 2012).
About PubCompare
Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.
We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.
However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.
Ready to get started?
Sign up for free.
Registration takes 20 seconds.
Available from any computer
No download required
Revolutionizing how scientists
search and build protocols!