Finnigan ltq

Manufactured by Thermo Fisher Scientific

Sourced in United States, Germany

The Finnigan LTQ is a linear ion trap mass spectrometer designed for high-performance qualitative and quantitative analysis. It features advanced ion optics, a high-capacity linear ion trap, and a fast scanning system to provide high sensitivity and resolution.

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Lab products found in correlation

Xcalibur 2 2 sp1, by Thermo Fisher Scientific (3 mentions) Lichrocart, by Merck Group (2 mentions) Betasil c18 column, by Thermo Fisher Scientific (2 mentions) Hp1100 hplc system, by Agilent Technologies (1 mentions) 1290 6545 uhplc qtof lc ms spectrometer, by Agilent Technologies (1 mentions) Jupiter c18, by Phenomenex (1 mentions) Mascot protein identification software, by Matrix Science (1 mentions) Hp 9000 workstation, by Agilent Technologies (1 mentions) Hp 1100 l, by Agilent Technologies (1 mentions) Ht multitron standard, by Infors (1 mentions) Vibra cell vcx130, by Sonics (1 mentions) Acquity uplc, by Waters Corporation (1 mentions) Finnigan trace dsq, by Thermo Fisher Scientific (1 mentions)

16 protocols using finnigan ltq

HPLC-APCI-MS Analysis of Isoxazolin-5-one Glucosides

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Analyses were carried out using an Agilent HP1100 HPLC system equipped with an RP-C18 column, LiChroCART (250 × 4 mm, 5 μm;Merck KGaA, 64271, Darmstadt, Germany) connected to a Finnigan LTQ (Thermo Electron Corp, Dreieich, Germany) operated in the APCI mode (vaporizer temperature: 500 °C, capillary temperature 300 °C). Standard compounds for identification were either purchased (Sigma-Aldrich (St. Louis, MO, USA) or synthesised. Isoxazolin-5-one glucoside and its esters were synthesised according to previously described protocols (Becker et al.2013 (link), 2015 (link)).
Samples were analyzed by injection (5 μl) and by the application of a gradient elution. The following protocol was used: 100 % solvent A (H₂O + 0.1 % HCOOH) and 0 % solvent B (MeCN + 0.1 % HCOOH), linear gradient to 60 % solvent B in 35 min. Extract samples of whole larvae were analyzed by injecting a 5 μl sample and using an isocratic elution with 35 % solvent B (v/v) in H₂O +0.1 % HCOOH. For identification and quantification, the formic acid adducts [M+HCOOH-H]⁻ were used (m/z 292 for 2-(β-D-glucopyranosyl)-3-isoxazolin-5-one (5), m/z 393 for 2-[6′-(3″-nitropropanoyl)-β-D-glucopyranosyl]-3-isoxazolin-5-one (6), m/z 331for salicin (3), and m/z 377 for 8-hydroxygeraniol-8-O-β-D-glucoside (1).

Pauls G., Becker T., Rahfeld P., Gretscher R.R., Paetz C., Pasteels J., von Reuss S.H., Burse A, & Boland W. (2016). Two Defensive Lines in Juvenile Leaf Beetles; Esters of 3-nitropropionic Acid in the Hemolymph and Aposematic Warning. Journal of Chemical Ecology, 42, 240-248.

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PFOS/PFOA Analysis in Whole Blood and Seminal Plasma

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Details for the analysis of PFOS/PFOA in whole blood were previously reported [29 (link),35 (link)]. Chemical analysis of seminal plasma was performed following the same procedure adopted for whole blood and yet tested for these and other body fluids [15 (link)]. Briefly, the samples were homogenized and extracted with methyl tert-butyl ether (MTBE, J.T. Baker, Center Valley, PA, USA). The solvent was evaporated under nitrogen and replaced with methanol (J.T. Baker). Quantification was performed in a HPLC (equipped with Betasil^© C18 column, Thermo Electron Corporation, San Jose, CA, USA) interfaced to a mass spectrometer at linear triple quadrupoles, by electrospray ionization (ESI) source, working in negative ion mode (Finnigan LTQ Thermo Electron Corporation, San Jose, CA, USA). The limit of detection (LOD) for both PFOS and PFOA were 0.4 ng/mL, corresponding to the value of the compounds in the blanks +3 Standard Deviation (SD).

La Rocca C., Tait S., Guerranti C., Busani L., Ciardo F., Bergamasco B., Perra G., Mancini F.R., Marci R., Bordi G., Caserta D., Focardi S., Moscarini M, & Mantovani A. (2015). Exposure to Endocrine Disruptors and Nuclear Receptors Gene Expression in Infertile and Fertile Men from Italian Areas with Different Environmental Features. International Journal of Environmental Research and Public Health, 12(10), 12426-12445.

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PFOS/PFOA Quantification by HPLC-MS/MS

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For analysis of PFOS/PFOA, an extraction was performed according to the analytical procedure previously described [32 ]. Briefly, the samples were extracted with methyl tert-butyl ether (MTBE, J.T. Baker). The solvent was evaporated under nitrogen and replaced with methanol (J.T. Baker). Twenty µL were injected into HPLC (equipped with Betasil© C18 column, Thermo Electron Corporation) interfaced to a mass spectrometer at linear triple quadrupoles, by an electrospray ionization (ESI) source, working in negative ion mode (Finnigan LTQ Thermo Electron Corporation, San Jose, CA). The limit of detection (LOD) for both PFOS and PFOA was 0.4 ng/mL, corresponding to the value of the compounds in the blanks +3 SD.

La Rocca C., Tait S., Guerranti C., Busani L., Ciardo F., Bergamasco B., Stecca L., Perra G., Mancini F.R., Marci R., Bordi G., Caserta D., Focardi S., Moscarini M, & Mantovani A. (2014). Exposure to Endocrine Disrupters and Nuclear Receptor Gene Expression in Infertile and Fertile Women from Different Italian Areas. International Journal of Environmental Research and Public Health, 11(10), 10146-10164.

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Analysis of Compounds 1 and 2 by HPLC-MS

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The analysis of compounds 1 and 2 was done by modifying the procedure that appears in the literature. 1 Measurements were carried out on an Agilent HP1100 HPLC system equipped with an OH-endcapped RP-C18 column (RP-C18e), LiChroCART (250 × 4 mm, 5 µm; Merck KGaA, 64271, Darmstadt, Germany) connected to a Finnigan LTQ (Thermo Electron Corp., Dreieich, Germany) ion trap mass spectrometer operating in the APCI mode (vaporizer temperature: 500 °C, capillary temperature 300 °C). Standard compounds for identification were either purchased (Sigma-Aldrich (St Louis, MO, USA)) or synthesized. 2 to 5 µl of the sample volume was injected, depending on the larval size (up to 20 mg larval fresh weight: 5 µl; m > 20 mg: 2 µl). The following parameters were used: flow rate = 0.5 ml min -1 at rt: 90% solvent A (H 2 O + 0.1% v/v HCO 2 H) and 10% solvent B (MeCN + 0.1% v/v HCO 2 H) for 5 minutes, linear gradient to 100% solvent B in 5 min, then 100% B for 2 min, linear gradient to 10% B in 5 min and further elution with 10% B for 5 min.

Becker T., Ploss K, & Boland W. (2016). Biosynthesis of isoxazolin-5-one and 3-nitropropanoic acid containing glucosides in juvenile Chrysomelina. Organic & biomolecular chemistry, 14(26).

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Thread Spray Ionization Mass Spectrometry

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Mass spectra were acquired on a Thermo Fisher Scientific Finnigan LTQ linear ion trap mass spectrometer (San Jose, CA, U.S.A.). The tip of the thread was positioned parallel to the MS inlet via a copper alligator clip, which was connected to an external high-voltage supply (0–6 kV). The thread spray ionization method generates ions without gas assistance so a close interface distance (0.5–5 mm) between the tip and the MS inlet was used to optimize signal intensity. MS parameters used were as follows: 200 °C capillary temperature, 3 microscans, and 60% S-lens voltage. Thermo Fisher Scientific Xcalibur 2.2 SP1 software was applied for MS data collecting and processing. Tandem MS with collision-induced dissociation (CID) was utilized for analyte identification. 1.5 Th (mass/charge units) for isolation window and 25 (manufacturer’s unit) of normalized collision energy was chosen for the CID tests.

Swiner D.J., Durisek GR I.I.I., Osae H, & Badu-Tawiah A.K. (2020). A proof-of-concept, two-tiered approach for ricin detection using ambient mass spectrometry. RSC Advances, 10(29), 17045-17049.

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NMR Spectroscopy and Mass Spectrometry Protocol

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¹H NMR and ¹³C NMR spectra
were recorded on a Bruker 400, 500, or 600 Hz instrument.
Data for ¹H NMR were presented as the chemical shift in
ppm, and multiplicities were denoted as follows: s, singlet; d, doublet;
t, triplet; q, quartet; m, multiplet; br, broad. Data for ¹³C NMR were reported as the chemical shift. The ESI mass spectra were
determined on a Thermo Fisher FINNIGAN LTQ instrument. All high-resolution
mass spectra (HRMS) results were obtained on an Agilent 1290-6545
UHPLC-QTOF LC/MS spectrometer. Thin-layer chromatography was performed
on silica gel plates (GF-254). DCM refers to dichloromethane. Flash
column chromatography was carried out using commercially available
200–300 mesh under pressure unless otherwise indicated. All
commercially available chemicals and solvents were directly used without
further purification unless otherwise noted.

Qin H., Odilov A., Bonku E.M., Zhu F., Hu T., Liu H., Aisa H.A, & Shen J. (2022). Facile Synthesis of Benzimidazoles via N-Arylamidoxime Cyclization. ACS Omega, 7(49), 45678-45687.

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Proteomics Analysis by Mass Spectrometry

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Extracts were electrophoresed on 4–20% gradient gels and then fixed. Each gel lane was cut lengthwise into a series of 1 mm slices and then reduced and alkylated followed by digestion with trypsin. Extracted peptides were analyzed by capillary LC-tandem MS (Thermo Finnigan LTQ) using a 50 μM i.d. × 8 cm long capillary column packed with Phenomenex Jupiter C18 reversed phase matrix resolved with a linear gradient of acetonitrile (13 (link)). The instrument was operated in the data-dependent mode in which one mass spectrum and eight collision induced dissociation spectra were acquired per cycle. Data were analyzed using Mascot protein identification software (Matrix Science, Ltd.), with manual inspection of peptide matches for validation.

MCGUIRE J.D., MOUSA A.A., ZHANG B.J., TODOKI L.S., HUFFMAN N.T., CHANDRABABU K.B., MORADIAN-OLDAK J., KEIGHTLEY A., WANG Y., WALKER M.P, & GORSKI J.P. (2014). EXTRACTS OF IRRADIATED MATURE HUMAN TOOTH CROWNS CONTAIN MMP-20 PROTEIN AND ACTIVITY. Journal of dentistry, 42(5), 626-635.

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Metabolite Profiling of Cell Cultures

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Samples were prepared by diluting 1 mL of spent cell culture medium with 4 mL of methanol (MeOH). Precipitated proteins and cell debris were removed by centrifugation at 14,000 x g at 4 °C for 20 min. The supernatant was evaporated to dryness under a stream of N₂, then resuspended in 100 μL 5 mM ammonium acetate (mobile phase). LCMS was performed using a Surveyor MS Pump Plus HPLC and Finnigan LTQ linear ion trap mass spectrometer (Thermo) as follows: separation of 75 μL of sample was performed with a linear gradient from 50% to 95.0% MeOH over 4 min after an initial run-in at 50% MeOH for 1 min on a 100 mm × 2 mm, 2.5 μm Synergi Hydro-RP reverse phase column (Phenomenex) with a flow rate of 200 μL·min⁻¹. The HPLC was next coupled to MS by negative electrospray ionization (-4.5 kV), and ion optics, gas flows, and capillary temperature were optimized using a direct injection of an aldosterone standard. The m/z transitions monitored were 359 > 189, 297, 315, and 331. Integrated peak areas were normalized to cellular protein content for analysis.

Maron B.A., Oldham W.M., Chan S.Y., Vargas S.O., Arons E., Zhang Y.Y., Loscalzo J, & Leopold J.A. (2014). Upregulation of Steroidogenic Acute Regulatory Protein by Hypoxia Stimulates Aldosterone Synthesis in Pulmonary Artery Endothelial Cells to Promote Pulmonary Vascular Fibrosis. Circulation, 130(2), 168-179.

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HPLC-DAD and ESI-MS Analysis of Compounds

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The quantitative analyses were carried out using a HP 1100 L liquid chromatograph equipped with a DAD detector and managed by a HP 9000 workstation (Agilent Technologies). A 150 mm × 4.6 mm i.d., 5 μm Zorbax Eclipse XDB, RP18 column was used. 20 μL of each sample were injected. Chromatography was carried out in gradient mode using a flow rate of 0.6 mL/min. The mobile phase was (A) formic acid/water pH 3.2 and (B) CH₃CN. The multi-step linear solvent gradient used was: 0–5 min 15–20% B; 5–7 min 20–30% B; 7–10 min 30–40% B; 10–15 min 40–50% B; 15–20 min 50–80% B; 20–25 min 80–15% B; post time 10 min; oven temperature 30 °C, flux: 0.6 mL/min. The UV/Vis spectra were recorded in the range 200–700 nm and the chromatograms were acquired at 210, 260, 280, 350 nm.
For qualitative analysis, MS experiments were conducted using a LTQ equipped with an ESI interface (Finnigan LTQ, Thermofisher Scientific, Waltham, MA). Mass spectrometry and electrospray operating parameters were optimized for negative polarity. The following final settings were used: sheath gas flow rate (arb): 30, aux gas flow rate (arb): 5, sweep gas rate (arb): 5, capillary temp (°C): 290.00, capillary voltage (V): 16.93, tube lents (V): −99.72.

Piazzini V., Monteforte E., Luceri C., Bigagli E., Bilia A.R, & Bergonzi M.C. (2017). Nanoemulsion for improving solubility and permeability of Vitex agnus-castus extract: formulation and in vitro evaluation using PAMPA and Caco-2 approaches. Drug Delivery, 24(1), 380-390.

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Thread Spray Ionization Mass Spectrometry

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Mass spectra were acquired on a Thermo Fisher Scientific Finnigan LTQ linear ion trap mass spectrometer (San Jose, CA, U.S.A.). The tip of the thread was positioned parallel to the MS inlet via a copper alligator clip, which was connected to an external high-voltage supply (0–6 kV). The thread spray ionization method generates ions without gas assistance so a close interface distance (0.5–5 mm) between the tip and the MS inlet was used to optimize signal intensity. MS parameters used were as follows: 200 °C capillary temperature, 3 microscans, and 60% S-lens voltage. Thermo Fisher Scientific Xcalibur 2.2 SP1 software was applied for MS data collecting and processing. Tandem MS with collision-induced dissociation (CID) was utilized for analyte identification. 1.5 Th (mass/charge units) for isolation window and 25 (manufacturer's unit) of normalized collision energy was chosen for the CID tests.

Swiner D.J., Durisek GR I.I.I., Osae H, & Badu-Tawiah A.K. (2020). A proof-of-concept, two-tiered approach for ricin detection using ambient mass spectrometry. RSC Advances, 10(29), 17045-17049.

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