Tested bee product (0.50 g) was mixed with 25 mL of bidistilled water and macerated for 24 h. The extracts were filtered through a paper filter (Labbox, Barcelona, Spain) and then through a 0.45 µm PVDF membrane filter. The UV-Vis spectra of bee products were performed using different dilution levels: bee pollen samples were diluted 11 times, beebread 18 times, royal jelly 10 times, propolis 19 times and dilution of three times was used for honey solutions. Absorbance values were recorded with a UV-visible spectrophotometer Shimadzu UV-Vis 1280 (Kyoto, Japan) using 1.0 nm scan pitch, 200–1100 nm scan range in 60 s. For all absorbance measurements Quartz cells (1 cm) were used [25 (link)].
Uv vis 1280
Manufactured by Shimadzu
Sourced in Japan
The UV-Vis 1280 is a single-beam ultraviolet-visible spectrophotometer manufactured by Shimadzu. It is designed to measure the absorption or transmission of light in the ultraviolet and visible regions of the electromagnetic spectrum.
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Lab products found in correlation
3 protocols using uv vis 1280
1
UV-Vis Analysis of Bee Products
2
Melanoidins Content Determination
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Absorbance at 420 nm was measured by UV–Vis spectrophotometer (UV–vis 1280, Shimadzu, Japan) as an indicator of melanoidins content in the MRPs. The sample solution was diluted 20 times before the analysis. The concentration of melanoidins was calculated based on the Lambert-Beer law. An extinction coefficient, i.e. 0.60 L/(mmol × cm), was utilized as reported in Kim [16] .
3
Quantification of Flavonoids and Phenols in Plant Extract
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The total flavonoid content in PH was determined by the aluminium chloride colorimetric method [22 (link)]. Briefly, 1 mL of PH or gallic acid (standard) of different concentrations was taken in screw cap test tubes. Then 3 mL of methanol, 200 μL of 10% aluminium chloride, 200 μL of 1 M potassium acetate, and 5.6 mL of distilled water were added to the test tubes respectively. Tubes were incubated for 30 min and read at 420 nm using a spectrophotometer (UV-VIS 1280, Shimadzu Corporation, Japan).
The content of total phenol (TPC) in PH was determined by the Folin-Ciocalteau reagent method [23 (link)]. A 0.5 mL of PH or gallic acid of different concentrations (800-50 μg/mL) was taken in a test tube. Then 2.5 mL of 10 times diluted Folin-Ciocalteau reagent solution was added. The resulting solution was added with 2.5 mL of 7.5% sodium carbonate. All the test tubes were then incubated for 20 min at 25 °C and absorbance was taken at 760 nm.
The content of total phenol (TPC) in PH was determined by the Folin-Ciocalteau reagent method [23 (link)]. A 0.5 mL of PH or gallic acid of different concentrations (800-50 μg/mL) was taken in a test tube. Then 2.5 mL of 10 times diluted Folin-Ciocalteau reagent solution was added. The resulting solution was added with 2.5 mL of 7.5% sodium carbonate. All the test tubes were then incubated for 20 min at 25 °C and absorbance was taken at 760 nm.
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