To generate whole genome shotgun sequencing libraries for D. pseudoobscura strains, we first pooled one male each from 8 SR strains and 8 ST strains from our Zion National Park collections. We extracted DNA from these flies using the 5 Prime Archive Pure DNA extraction kit according to the manufacturer’s protocol (ThermoFisher, Waltham, MA). All libraries were generated with the Illumina TruSeq Nano kit (Epicentre, Illumina Inc., CA) using the manufacturers protocol, and sequenced as 500 bp paired end reads on an Illumina HiSeq 2000 instrument.
5 prime archive pure dna extraction kit
Manufactured by Thermo Fisher Scientific
The 5 Prime Archive Pure DNA extraction kit is a laboratory tool designed to isolate and purify DNA from a variety of sample types. It utilizes a silica-based membrane technology to efficiently capture and concentrate DNA fragments, allowing for high-quality DNA extraction.
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Lab products found in correlation
2 protocols using 5 prime archive pure dna extraction kit
1
Pooled Whole Genome Sequencing of Drosophila pseudoobscura
2
Whole Genome Sequencing of D. persimilis
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To generate whole genome shotgun sequencing libraries for D. persimilis strains, we pooled one male each from two SR strains and two ST strains (from Sierra Nevada and Mt St Helena collections). We extracted DNA from these flies using the 5 Prime Archive Pure DNA extraction kit according to the manufacturer’s protocol (ThermoFisher, Waltham, MA). All libraries were generated with the Illumina TruSeq Nano kit (Epicentre, Illumina Inc, CA) using the manufacturers protocol, and sequenced as 500bp paired end reads on an Illumina HiSeq 2000 instrument.
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