1250 infinity system

The WSFs (3 mg/mL) from different ripening points were analyzed by high-performance size-exclusion chromatography coupled with a refractive index detector (HPSEC-RID) using a 1250 Infinity system (Agilent, Santa Clara, CA, USA). The system was equipped with four PL aquagel-OH columns (60, 50, 40 and 30; 429 300 × 7.5 mm) connected in series. The eluent used was 0.2 M NaNO₃/0.02% NaN₃ (0.6 mL/min). The RID temperature was set at 30 °C^{20 (link)}. Average molecular weight (Mw) was calculated using a standard curve of dextrans (Mw 5–1,800 kDa; Sigma-Aldrich (St. Louis, MO, USA)). The void volume (V_o) was the elution time of the heavier molecule (blue dextran; ~1,800 kDa), and the included volume (V_i) was the elution time of glucose.

Molecular mass distribution of the papaya WSF was analyzed by high performance size exclusion chromatography coupled to a refractive index detector (HPSEC-RID) using a 1250 Infinity system (Agilent, Santa Clara, CA). Samples were diluted with water (1 mg/mL), injected (25 µL) and separation was conducted through four PL aquagel-OH MIXED-M (300 × 7.5 mm, 8 μm) columns in tandem (Agilent, Santa Clara, CA). The eluent was 0.2 M NaNO₃ at 35 °C with a flow of 0.6 mL·min⁻¹. Molecular weights were estimated using dextran T-series (5, 25, 50, 80, 150 and 410 kDa; Fluka^TM) as external standards.