Pharmaceutical grade hyaluronic acid (HA) sodium salt from Streptococcus Equi (MW = 40, 200, 600 kDa and 2 MDa) was obtained from Lehvoss Iberia (Barcelona, Spain). Silver nitrate, Muller-Hinton Broth (MHB), Coliform ChromoSelect agar, Braid-Parker agar, Dulbecco’s modified Eagle’s medium—high glucose (DMEM) were supplied by Sigma-Aldrich (Madrid, Spain). Invitrogen Life Technologies Corporation (Sant Cugat del Vallès, Spain) provided the alamarBlue cell viability reagent. Adipic acid dihydrazide (ADH), hydrochloric acid, nitric acid, 1-ethyl-3-(3-dimethylaminopropyl)carbodiimide (EDC) and 2,4,6-trinitrobenzene sulfonic acid (TNBSA) were supplied by Thermo Fischer Scientific (Sant Cugat del Vallès, Spain). Sodium dodecyl sulfate was purchased from Fisher Bioreagent (Madrid, Spain). Bacterial strains Staphylococcus aureus (ATCC 25923) and Escherichia coli (ATCC 25922), and human fibroblast (ATCC-CRL-4001, BJ-5ta) and keratinocyte (HaCaT cell line) cells were obtained from the American Type Culture Collection (ATCC LGC Standards, Barcelona, Spain).
Coliform chromoselect agar
Manufactured by Merck Group
Sourced in Spain
Coliform ChromoSelect agar is a selective and differential medium used for the detection and enumeration of coliform bacteria in water, food, and other samples. It contains chromogenic substances that allow the differentiation of coliforms from other bacteria based on colony color.
Automatically generated - may contain errors
Lab products found in correlation
Alamarblue cell viability reagent, by Thermo Fisher Scientific (2 mentions)
Hacat cell line, by American Type Culture Collection (2 mentions)
Nitric acid, by Thermo Fisher Scientific (1 mentions)
Dulbecco s modified eagle s medium dmem high glucose, by Merck Group (1 mentions)
1 ethyl 3 3 dimethylaminopropyl carbodiimide edc, by Thermo Fisher Scientific (1 mentions)
2 4 6 trinitrobenzene sulfonic acid tnbsa, by Thermo Fisher Scientific (1 mentions)
Muller hinton broth mhb, by Merck Group (1 mentions)
Silver nitrate, by Merck Group (1 mentions)
Escherichia coli atcc 25922, by American Type Culture Collection (1 mentions)
Staphylococcus aureus atcc 25923, by American Type Culture Collection (1 mentions)
Cetrimide agar, by Merck Group (1 mentions)
Sodium tellurite, by Merck Group (1 mentions)
Milli q plus system, by Merck Group (1 mentions)
Chloroform, by Merck Group (1 mentions)
Phosphate buffered saline (pbs), by Merck Group (1 mentions)
Dulbecco s modified eagle s medium, by Merck Group (1 mentions)
P aeruginosa, by American Type Culture Collection (1 mentions)
Bj 5ta, by American Type Culture Collection (1 mentions)
2 protocols using coliform chromoselect agar
1
Hyaluronic Acid Characterization and Antimicrobial Evaluation
2
Antimicrobial Potential of Kraft Lignin
Check if the same lab product or an alternative is used in the 5 most similar protocols
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Protobind 6000
sulfur-free Kraft lignin powder with an average molecular weight of
1000 g·mol–1 was purchased from Green Value
(Switzerland). Sodium tellurite, chloroform, phosphate-buffered saline
(PBS), Coliform ChromoSelect agar, Cetrimide agar, and Dulbecco’s
modified Eagle’s medium (DMEM) were obtained from Sigma-Aldrich
(Spain). Nutrient broth (NB) was provided by Sharlab (Spain). AlamarBlue
cell viability reagent and molecular probe 2′,7′-dichlorodihydrofluorescein
diacetate (H2DCFDA) were purchased from Invitrogen, Life
Technologies Corporation (Spain). Avanti Polar Lipids provided phosphatidylethanolamine
(PE, #840027) and phosphatidylglycerol (PG, #841188) extracted from E. coli. Bacterial strains S. aureus (ATCC 25923), E. coli (ATCC 25922),
and P. aeruginosa (ATCC 10145), human
fibroblast cells (ATCC-CRL-4001, BJ-5ta), and human keratinocyte cells
(HaCaT cell line) were obtained from the American Type Culture Collection
(ATCC LGC Standards, Spain). The water used in all experiments was
purified by the Milli-Q plus system (Millipore) with 18.2 MΩ·cm–1 resistivity prior to its use.
sulfur-free Kraft lignin powder with an average molecular weight of
1000 g·mol–1 was purchased from Green Value
(Switzerland). Sodium tellurite, chloroform, phosphate-buffered saline
(PBS), Coliform ChromoSelect agar, Cetrimide agar, and Dulbecco’s
modified Eagle’s medium (DMEM) were obtained from Sigma-Aldrich
(Spain). Nutrient broth (NB) was provided by Sharlab (Spain). AlamarBlue
cell viability reagent and molecular probe 2′,7′-dichlorodihydrofluorescein
diacetate (H2DCFDA) were purchased from Invitrogen, Life
Technologies Corporation (Spain). Avanti Polar Lipids provided phosphatidylethanolamine
(PE, #840027) and phosphatidylglycerol (PG, #841188) extracted from E. coli. Bacterial strains S. aureus (ATCC 25923), E. coli (ATCC 25922),
and P. aeruginosa (ATCC 10145), human
fibroblast cells (ATCC-CRL-4001, BJ-5ta), and human keratinocyte cells
(HaCaT cell line) were obtained from the American Type Culture Collection
(ATCC LGC Standards, Spain). The water used in all experiments was
purified by the Milli-Q plus system (Millipore) with 18.2 MΩ·cm–1 resistivity prior to its use.
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