Rat anti mouse il 10 clone jes5 16e3

An ELISA for the detection of IFN-γ or IL-10 in plasma was carried out as described previously [18 (link)]. A rat anti-mouse IFN-γ mAb (clone R4–6A2; eBioscience, San Diego, CA, USA) and a rat anti-mouse IL-10 (clone JES5–16E3; eBioscience) were used as the capture antibodies, and a biotinylated, rat anti-mouse IFN-γ mAb (clone XMG1.2; eBioscience) and rat anti-mouse IL-10 mAb (clone JES5–2A5; eBioscience) as the detecting antibodies. The concentrations of cytokines in plasma were calculated from standard curves prepared using known quantities of murine recombinant IFN-γ (Genzyme, Boston, MA, USA) and IL-10 (Pierce, Rockford, IL, USA). The reaction was visualized using peroxidase-conjugated streptavidin (Zymed) and the substrate 2,2’-azino-bis(3-etylbenzthiazoline-6-sulphonic acid) (Wako, Osaka, Japan).

Rat Anti-Mouse IFNγ clone XMG1.2, Invitrogen, Cat #: 12-7311-82, PE (1 μL/1 × 10⁶ cells).
Rat Anti-Mouse TNFα clone MP6-XT22, eBioscience, Cat#17-7321-82, APC (1.25 μL/1 × 10⁶ cells).
Rat Anti-Mouse IL2 clone JES6-5H4, eBioscience, Cat#48-7021-82, eFluor450 (1.25 μL/1 × 10⁶ cells).
Rat Anti-Mouse IL4 clone 11B11, BioLegend, Cat#504132, PE-Dazzle594 (1.25 μL/1 × 10⁶ cells).
Rat Anti-Mouse IL10 clone JES5-16E3, eBioscience, Cat#56-7101-82, AF700 (1.25 μL/1 × 10⁶ cells).
Rat Anti-Mouse CD4 clone GK1.5, BioLegend, Cat#100451, BV605 (2.5 μL/1 × 10⁶ cells).
Rat Anti-Mouse CD8 clone 53-6.7, BD, Cat#563332, BV786 (0.75 μL/1 × 10⁶ cells).
Live/dead stain, eBioscience Cat#65-0866-18, eFluor506 (1:1000).