Hionic fluor complete lsc cocktail

After treatment with NANA or fenofibrate for 6 weeks, mice were injected with [³H]-cholesterol-loaded macrophages. Thereafter, blood (100 μL) was sampled from the retro-orbital sinus at 0, 12, 24 and 48 h, and faeces were collected every 24 h. The animals were euthanized, and the livers were collected.
Liver (0.3 g) or faeces were homogenized in a hexane/isopropanol (3:2, v/v) solution, and the mixtures were placed on a shaker for 12 h at room temperature. Thereafter, the mixture was centrifuged at 5000×g for 15 min, and the supernatant was kept for measurement. Prepared samples were transferred into 5.0 mL scintillation vials, and 4.0 mL of HIONIC FLUOR™ complete LSC-cocktail (PerKinElmer, Inc., Waltham, MA, USA) was then added and mixed well. The scintillation vials were counted on a SN-6930B type liquid scintillation counter (BECKMAN, USA). The results were expressed as a percentage of injected [³H]-cholesterol or directly by counts per minute (CPM) [15 (link)].

After 8 weeks of treatment, the other 6 mice in each group were used for isotope tracing study. After the injection of [³H]-cholesterol-loaded macrophages, blood was sampled from the retro-orbital sinus at the time points of 0, 6, 12 and 24 h, 100 μL per mouse each time. Faeces were collected every 24 h. Blood was sampled at 48 h after the injection, as described above. Animals were killed, and the liver and bile were collected.
Liver (0.3 g) and faeces were homogenized in a hexane/isopropanol (3:2, v/v) solution, and the mixtures were placed on a shaker for 12 h at room temperature. Then, the mixture was centrifuged at 5000 × g for 15 min, and the supernatant was kept for measurement. Prepared samples were transferred into 5.0 mL scintillation vials, and 4.0 mL of HIONIC FLUOR™ complete LSC-cocktail (PerKinElmer, Inc., Waltham, MA, USA) was then added and mixed well. The scintillation vials were counted on a SN-6930B type liquid scintillation counter (BECKMAN, USA). The results were expressed as a percentage of injected [³H]-cholesterol or directly by counts per minute (CPM).