Apc labeled anti cd3

Manufactured by BioLegend

Sourced in United States

APC-labeled anti-CD3 is a monoclonal antibody conjugated with Allophycocyanin (APC), which binds to the CD3 antigen expressed on the surface of T cells. This product is suitable for flow cytometry applications to identify and quantify T cell populations.

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Lab products found in correlation

Fitc labeled anti cd4, by BioLegend (2 mentions) Percp cy5.5 labeled anti cd8, by Thermo Fisher Scientific (2 mentions) Bv510 labeled anti cd44, by BioLegend (2 mentions) Fixable viability stain 780, by BD (2 mentions) Apc labeled anti cd3, by Thermo Fisher Scientific (1 mentions) Pe labeled anti cd80, by BioLegend (1 mentions) Fitc labeled anti cd4, by Thermo Fisher Scientific (1 mentions) Gallios flow cytometer, by Beckman Coulter (1 mentions) Apc brdu flow kit, by BD (1 mentions)

Close spelling variants of this product

Anti-CD3-APC CD3-APC Anti-CD3

4 protocols using apc labeled anti cd3

Flow Cytometric Analysis of Rat T Cells and DCs

Cited 1 time

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Analysis of BTLA expression on CD4+ lymphocytes in peripheral blood mononuclear cells (PBMCs) of patients from the BPAR and stable groups was performed as previously described^{27 (link)}. Peripheral blood cells of rat recipients at D3 and D7 following kidney transplantation were collected and then stained with APC-labeled anti-CD3 (eBioscience CA, USA), FITC-labeled anti-CD4 (eBioscience CA, USA), and PercCP-eFluor710-labeled anti-CD8 (eBioscience CA, USA) antibodies at 4 °C for 45 min for flow cytometry analysis. APC-labeled anti-OX62 (eBioscience CA, USA) along with PE-labeled anti-CD80 (BioLegend CA, USA) were used to determine the purity of the primary DCs, and APC-labeled anti-CD3 was used for T cells. Cell staining was performed according to the manufacturers’ recommendations. T cell proliferation was measured by bromodeoxyuridine (BrdU) incorporation (APC BrdU Flow Kit, BD Pharmingen NJ, USA) according to the manufacturer’s protocols. Cells stained with APC-labeled anti-BrdU mAb (BD Pharmingen NJ, USA) were analyzed by flow cytometry. Flow cytometry analysis was conducted using a Gallios flow cytometer (Beckman Coulter, USA).

Zhang J., Zhang H., Wang Z., Yang H., Chen H., Cheng H., Zhou J., Zheng M., Tan R, & Gu M. (2019). BTLA suppress acute rejection via regulating TCR downstream signals and cytokines production in kidney transplantation and prolonged allografts survival. Scientific Reports, 9, 12154.

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MHC-II Tetramer Assay for Lung CD4 T Cells

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The PE-labeled MHC-II Ag85B tetramer I-A(b)/FQDAYNAAGGHNAVF and control tetramer I-A(b)/PVSKMRMATPLLMQA were provided by the NIH Tetramer core facility at Emory University (Atlanta, GA). Isolated PBMCs, splenocytes, and lung lymphocytes were incubated with fixable viability stain 780 (BD Biosciences), APC-labeled anti-CD3 (Biolegend), PerCP-Cy5.5-labeled anti-CD8 (eBioscience), FITC-labeled anti-CD4 (Biolegend), and BV510-labeled anti-CD44 (Biolegend), together with either control or Ag85B PE-labeled MHC-II tetramer. To detect tissue-resident CD4 T cells in the lung, lung lymphocytes were incubated with the same cocktails, together with APC-Cy7-labeled anti-CD69 (Biolegend). Cells were washed three times with PBS/2% PBS. Sample acquisition was performed on FACSCelesta and data analyzed using FlowJo.

Kirk N.M., Huang Q., Vrba S., Rahman M., Block A.M., Murphy H., White D.W., Namugenyi S.B., Ly H., Tischler A.D, & Liang Y. (2023). Recombinant Pichinde viral vector expressing tuberculosis antigens elicits strong T cell responses and protection in mice. Frontiers in Immunology, 14, 1127515.

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Detect Antigen-Specific T Cells

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The PE-labeled MHC-I tetramer H-2K(b)/EsxH epitope IMYNYPAM was provided by the NIH Tetramer core facility at Emory University (Atlanta, GA). Isolated PBMCs, splenocytes, and lung lymphocytes were incubated with PE-labeled EsxH/H-2K(b) MHC-I tetramer, fixable viability stain 780 (BD Biosciences), APC-labeled anti-CD3 (Biolegend), PerCP-Cy5.5-labeled anti-CD8 (eBioscience), FITC-labeled anti-CD4 (Biolegend), and BV510-labeled anti-CD44 (Biolegend) for one hour at room temperature. Cells were washed three times with PBS/2% PBS. Sample acquisition was performed on FACSCelesta and data analyzed using FlowJo.

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Multiparametric PBMC Phenotyping by FACS

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PBMC were obtained from whole blood after dilution in an equal volume of
Dulbecco’s phosphate buffered saline (DPBS) and overlaid onto
Ficoll-Paque Plus. Whole blood was centrifuged at 400G for 30 min at 20°C
with no brake. Mononuclear cells from the interphase between plasma and Ficoll
were harvested, washed twice in DPBS and subjected to RBS lysis using ACK. After
a final wash, the supernatant was discarded, and the pellet was resuspended in
cold FACS buffer (DPBS containing 2% BSA and 2mM EDTA).
PBMC were analyzed by fluorescence activated cell sorting (FACS) to
determine phenotype. Briefly, cells were stained with Fixable Viability Dye Blue
to ascertain viability (ThermoFisher), BV510-labeled anti-CD45 (BioLegend),
APC-labeled anti-CD3 (BioLegend), A700-labelled anti-CD14 and anti-CD19
(BioLegend), APC-Cy7 labeled anti-CD16 (BioLegend), BV605-labeled anti-CD56
(BioLegend), BV786-labeled anti-CD4 (BD Bioscience), BB700-labeled anti-CD8a (BD
Bioscience), BB515-labeled anti-Tim-3 (BD Biosciences), PE-labeled anti-TIGIT
(eBio), and BV421-labeled anti-PD-1 (BioLegend). Cytometric analysis was
performed on a BD LSR Fortessa X-20 and data analyzed with FlowJo v10
(Treestar). For the analysis, cells were gated on live singlets followed by
relevant lineage markers. Isotype control antibodies were included in each
experiment to determine appropriate gating for Tim-3, TIGIT, and PD-1.

AUDENET F., FARKAS A.M., ANASTOS H., GALSKY M.D., BHARDWAJ N, & SFAKIANOS J.P. (2018). Immune phenotype of peripheral blood mononuclear cells in patients with high-risk non-muscle invasive bladder cancer. World journal of urology, 36(11), 1741-1748.

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