Microvue complement c3a plus

Manufactured by Quidel

The Microvue Complement C3a Plus is a laboratory instrument designed for the quantitative measurement of the C3a complement component in biological samples. It utilizes an enzyme-linked immunosorbent assay (ELISA) method to detect and quantify the levels of C3a in the tested samples.

Automatically generated - may contain errors

Lab products found in correlation

Meclofenamate, by Merck Group (3 mentions) Txb2 eia kit, by Cayman Chemical (1 mentions) Ctad tubes, by BD (1 mentions) Maxisorp 96 well flat bottom plates, by Thermo Fisher Scientific (1 mentions) Spectramax m3, by Molecular Devices (1 mentions)

3 protocols using microvue complement c3a plus

Measuring Coagulation and Inflammation Markers

Cited 1 time

Check if the same lab product or an alternative is used in the 5 most similar protocols

Beta-thromboglobulin (βTG) and prothrombin fragments 1+2 (F1+2) were measured by commercial enzyme-linked immunosorbent assay (ELISA) in plasma samples collected in CTAD tubes as previously described (24 (link)). EDTA plasma was used to measure histamine (ELISA kit Starfish, Gernusco, Italy) and C3a levels. Because the manufacturer of C3a kits recently modified their ELISA to the C3a Plus version (Microvue Complement C3a Plus, Quidel, San Diego, CA), archived GalTKO.hCD46 samples were reanalyzed for this study. Blood, collected at 3 time points (0, 15 and 60min) in EDTA tubes containing 100μl (at 10μg/ml) of meclofenamate (Sigma-Aldrich, St.Louis, MO), was analyzed to measure plasma levels of thromboxane B₂ (TXB2 EIA Kit, Cayman Chemical Company, Ann Arbor, MI; Catalog No. 519031).

Burdorf L., Riner A., Rybak E., Salles I., De Meyer S., Shah A., Quinn K., Harris D., Zhang T., Parsell D., Ali F., Schwartz E., Kang E., Cheng X., Sievert E., Zhao Y., Braileanu G., Phelps C., Ayares D., Deckmyn H., Pierson R I.I.I, & Azimzadeh A. (2016). Platelet sequestration and activation during GalTKO.hCD46 pig lung perfusion by human blood is primarily mediated by GPIb, GPIIb/IIIa, and VWF. Xenotransplantation, 23(3), 222-236.

+ Open protocol

+ Expand

Plasma Biomarkers of Thrombosis and Inflammation

Cited 1 time

Check if the same lab product or an alternative is used in the 5 most similar protocols

Beta-thromboglobulin (βTG), prothrombin fragments 1+2 (F1+2), were measured by commercial enzyme-linked immunosorbent assay (ELISA) in plasma samples collected in CTAD tubes as previously described (31 EDTA plasma was used to measure histamine (ELISA kit Starfish) and C3a levels (Microvue Complement C3a Plus, Quidel) (29 (link)). Blood, collected in EDTA tubes containing 100μl of meclofenamate (10μg/ml, Sigma-Aldrich), was used for thromboxane (Thromboxane B2 EIA Kit).

Burdorf L., Harris D., Dahi S., Zhang T., Ali F., Shah A., Thompson M., Braileanu G., Cheng X., Sievert E., Schwartz E., Sendil S., Parsell D.M., Redding E., Phelps C.J., Ayares D.L., Azimzadeh A.M, & Pierson RN 3.r.d. (2018). THROMBOXANE AND HISTAMINE MEDIATE PVR ELEVATION DURING XENOGENEIC PIG LUNG PERFUSION WITH HUMAN BLOOD. Xenotransplantation, 26(2), e12458.

+ Open protocol

+ Expand

Biomarkers of Thrombosis and Inflammation

Check if the same lab product or an alternative is used in the 5 most similar protocols

Beta-thromboglobulin (βTG) and prothrombin fragments 1+2 (F1+2), were measured by commercial enzymelinked immunosorbent assay (ELISA) using plasma samples collected in CTAD tubes (Becton Dickinson, Franklin Lakes, NK, USA). EDTA plasma was used to measure histamine (ELISA kit Starfish) and C3a levels (Microvue Complement C3a Plus, Quidel). Blood, collected in EDTA tubes containing 100 μL of meclofenamate (10 μg/ml, Sigma-Aldrich), was used for thromboxane (Thromboxane B2 EIA Kit).
Additionally, human blood serum samples were tested for anti-Neu5Gc IgG antibodies using Maxisorp 96-well flat bottom plates (Nunc, cat #44-2404-21) coated with 1 μg/ml of Neu5Gc-PAA (Glycotech, cat #08-051) or PAA (Glycotech, cat #08-000). Affinity purified horseradish peroxidase (HRP) -conjugated goat anti-human IgG (Jackson, cat #109-035-008) was used to detect the anti-Neu5Gc IgG antibodies. All ELISA plates were measured using Spectramax M3 (Molecular Devices).

Chaban R., Habibabady Z., Hassanein W., Connolly M.R., Burdorf L., Redding E., Laird C., Ranek J., Braileanu G., Sendil S., Cheng X., Sun W., O'Neill N.A., Kuravi K., Hurh S., Ayares D.L., Azimzadeh A.M, & Pierson RN 3.r.d. (2022). Knock-out of N-glycolylneuraminic acid attenuates antibody-mediated rejection in xenogenically perfused porcine lungs. Xenotransplantation, 29(6).

+ Open protocol

+ Expand

About PubCompare

Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.

We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.

However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.

Ready to get started?

Revolutionizing how scientists
search and build protocols!