Uv 2700 uv vis spectrometer

The adsorption ability of 1 was evaluated by adsorbing pure QT in ethanol solution at ambient temperature. Typically, 5 mg of activated 1 was soaked in a 2 mL 40 mg L⁻¹ quercetin ethanol solution for 24 h. The concentration of QT at a given time was measured by UV-vis spectrophotometer from 200 nm to 500 nm on a SHIMADZU UV-2700 UV-vis spectrometer. Absolute ethanol was used as reference in this experiment. A calibration curve (eqn (S1)†) was plotted to calculate the maximum adsorption amount of QT by 1.

The glucose-tolerant Synechocystis sp. PCC 6803 strain (hereafter referred to as the GT strain) is isolated by Williams in 1988 (Williams 1988 (link)). Synechocystis 6803 was cultivated in 70 mL of modified BG-11 medium, consisting of BG-11₀ liquid medium (Rippka 1988 (link)) supplemented with 20 mM HEPES-KOH (pH 7.8) and 5 mM NaNO₃ or l-arginine. For the incubation, the cultures were bubbled with 1% (v/v) CO₂ in the air and incubated at 30 °C under continuous white light (50 μmol photons m^− 2 s^− 1). For the cultivation of the mutant strains overexpressing argG or argH, 70 mL of modified BG-11 medium containing 0.7 μg/mL kanamycin was used. The OD₇₃₀ of the cyanobacterial cultures was measured using a Shimadzu UV-2700 UV-vis spectrometer (Shimadzu, Kyoto, Japan). The OD₇₃₀ was set at 0.4 when the cultivation was started.

All the reagents used in this work were commercially available and used as received without further purification. Powder X-ray diffraction (PXRD) patterns were recorded using a Rigaku (D/Max-Ultima IV) diffractometer equipped with Cu Kα radiation (λ = 1.54184 Å). Simulated PXRD patterns were calculated with the Mercury program using the single-crystal data.^{16 (link)} Thermogravimetric analysis (TGA) was performed using a Netzsch STA449F3 instrument in N₂ atmosphere in the temperature range of 30–800 °C and a heating rate of 10 °C min⁻¹. IR spectra in the range of 500–4000 cm⁻¹ was collected using a SHIMADZU IRTracer-100 spectrometer. The ESI-MS was carried out on a Thermo TSQ instrument. UV-vis study was performed on a SHIMADZU UV-2700 UV-vis spectrometer at room temperature. Transmission electron microscope energy-disperse X-ray spectroscopy (TEM-EDS) was conducted on a Hillios G4 UX transmission electron microscope. Crystals of 1·guest were evacuated with supercritical CO₂ in a Tousimis™ Samdri® PVT-30 critical point dryer prior to gas sorption measurements. Low-pressure gases (N₂ and CO₂) sorption isotherms (up to 1 atm) were performed on Micromeritics ASAP 2020 surface area.