Ema clone e29

Manufactured by Roche

The EMA (clone E29) is a laboratory equipment product offered by Roche. It is a monoclonal antibody that can be used for the detection and analysis of certain cellular markers. The core function of the EMA (clone E29) is to provide a tool for researchers and clinicians to study and characterize specific cell types or cellular processes. However, a more detailed and unbiased description of the product's features and intended use cannot be provided while maintaining the requested approach.

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Lab products found in correlation

Bond polymer refine detection, by Leica camera (1 mentions) Clone ae1 ae3, by Agilent Technologies (1 mentions) Optiview dab ihc detection kit, by Roche (1 mentions) Leica bond 3, by Leica camera (1 mentions) Benchmark ultra, by Roche (1 mentions) Ck7 clone ov tl 12 30, by Agilent Technologies (1 mentions) Cd10 clone sp67, by Roche (1 mentions) Benchmark xt automated system, by Roche (1 mentions) Ventana discovery system, by Roche (1 mentions) Diaminobenzidine, by Agilent Technologies (1 mentions) S100 protein clone poly z311, by Agilent Technologies (1 mentions) Myod1 clone ep212, by Cell Marque (1 mentions) Cd4 4b12, by Leica Biosystems (1 mentions) Etv4 clone 16, by Santa Cruz Biotechnology (1 mentions) Desmin clone de r 11, by Roche (1 mentions) Cd30 ber h2, by Agilent Technologies (1 mentions) Benchmark ultra automated stainer, by Roche (1 mentions)

3 protocols using ema clone e29

Immunohistochemical Staining Protocols for Pathological Analysis

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The immunohistochemical stains were performed at the Department of Pathology, MSKCC, using commercially available antibodies. Staining for pan-cytokeratin (clone AE1/AE3, Dako, 1:1600), EMA (clone E29, Ventana, pre-diluted), S100 (polyclonal, Dako, 1:8000), INI-1/BAF-47 (clone 25/BAF47, BD Bioscience, 1:200) were performed on the automated Ventana BenchMark ULTRA immunostainer (Roche, Indianapolis, IN), using OptiView DAB IHC Detection Kit (Ventana Medical Systems, Tucson, AZ). Staining for Brachyury (clone EPR18113, Abcam, 1:500) was performed on the Leica Bond-3 immunostainer (Leica, Buffalo Grove, IL), using a polymer detection system (DS9800; Leica, Bond Polymer Refine Detection).

Wen X., Cimera R., Aryeequaye R., Abhinta M., Athanasian E., Healey J., Fabbri N., Boland P., Zhang Y, & Hameed M. (2021). Recurrent loss of chromosome 22 and SMARCB1 deletion in extra-axial Chordoma: A clinicopathological and molecular analysis. Genes, chromosomes & cancer, 60(12), 796-807.

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Immunohistochemical Profiling of Tissue Samples

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Immunohistochemistry was conducted in 5 μm formalin-fixed
paraffin-embedded (FFPE) whole tissue sections. Staining for CK7 (clone OV-TL
12/30, DAKO, 1:800), AMACR (clone 12H4, Zeta Corp, 1:100), CD10 (clone SP67,
Ventana), EMA (clone E29, Ventana), CD15 (clone MMA, Ventana) and Pax8
(Proteintech, 1:100) was performed using a BenchMark XT automated system
(Ventana, Tucson, AZ). Staining for YAP/TAZ (D24E4, Cell Signaling Technology,
1:50) was performed using an automated Ventana Discovery system (Ventana).
Immunostaining scores (H-scores) for YAP/TAZ nuclear staining were determined as
[H= intensity (0–3) x percentage of positive cells (1–100)].

Ren Q., Wang L., Al-Ahmadie H.A., Fine S.W., Gopalan A., Sirintrapun S.J., Tickoo S.K., Reuter V.E, & Chen Y.B. (2018). Distinct Genomic Copy Number Alterations Distinguish Mucinous Tubular and Spindle Cell Carcinoma of the Kidney from Papillary Renal Cell Carcinoma with Overlapping Histologic Features. The American journal of surgical pathology, 42(6), 767-777.

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Immunohistochemical Profiling of Tissue Samples

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The tissue slides were deparaffinized in xylene, hydrated in alcohol, and baked in a microwave (30 min in Tris buffer pH 9). Endogenous peroxidase was blocked. Staining was performed on the Benchmark ultra-automated stainer (Ventana) using diamino-benzidine as chromogen (Dako, Glostrup, Denmark). The following antibodies were used: AE1/E3 (clone PCK26, Ventana), KL1 (clone KL1, HIS-TOLS reagent), EMA (clone E29, Ventana), P63 (4A4, Ventana), S100 protein (clone poly Z311, Dako), SOX10 (EP268, BioSB), Desmin (clone DE-R11, Ventana), Myogenin (clone LO26, Leica), MYOD1 (clone EP212, Cell marque), ALK (clones D5F3, Cell Signaling Technology and clone 1A4, Novocastra), CD99/MIC2 (12E7, Dako), ETV4 (clone 16, Santa Cruz), CD30 (BerH2, Dako), CD4 (4B12, Novocastra), and CD7 (SP94, Ventana).

Le Loarer F., Cleven A.H.G., Bouvier C., Castex M.P., Romagosa C., Moreau A., Salas S., Bonhomme B., Gomez-Brouchet A., Laurent C., Le Guellec S., Audard V., Giraud A., Ramos-Oliver I., Cleton-Jansen A.M., Savci-Heijink D.C., Kroon H.M., Baud J., Pissaloux D., Pierron G., Sherwood A., Coindre J.M., Bovée J.V.M.G., Larousserie F, & Tirode F. (2020). A subset of epithelioid and spindle cell rhabdomyosarcomas is associated with TFCP2 fusions and common ALK upregulation. Modern pathology : an official journal of the United States and Canadian Academy of Pathology, Inc, 33(3).

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