Tg slc1a3 cre ert 1nat j

Mouse husbandry and procedures, including euthanasia, complied with the Guide for the Care and Use of Laboratory Animals of the National Institutes of Health and were approved by the Washington University in St. Louis Institutional Animal Care and Use Committee (Protocol # 19-0950). Mouse strains included C57BL/6J (The Jackson Laboratory, Stock #00664), BKS.Cg-Dock7^m +/+ Lepr^db/J (“db/db”, The Jackson Laboratory, Stock #00642), Tg(Slc1a3-cre/ERT)1Nat/J (“GLAST-CreER”, The Jackson Laboratory, Stock #012586), and B6.Cg-Gt(ROSA)26Sor^{tm14(CAG-tdTomato)Hze}/J (“tdTomato” The Jackson Laboratory, Stock #007914) [20 (link),21 (link),22 (link)]. For in vivo luciferase assays, we used a peroxisome proliferator response element luciferase transgenic mouse on the C57/BL/6J background (repTOP PPRE-Luc, Charles River), as previously described [23 (link),24 (link)]. After weaning, all mice were maintained on a standard chow diet until 3 months of age. At 3 months, mice were maintained on standard chow or transitioned to a custom-milled standard chow diet supplemented with 0.2% w/w fenofibrate (Envigo Teklad rodent diet) as previously described [25 (link)].

hCD63-GFP floxed mice were generated in the lab by homologous recombination, as previously described^{35 (link)}. The WT (C57B/6J, #000664), Ai14-tdT^f/f reporter (#007914), Tg(Slc1a3-cre/ERT)1Nat/J (#012586), ApoE-KO (B6.129P2-Apoe tm1Unc/J #002052), B6.Cg-Tg (Thy1-YFP)HJrs/J (#003782), and B6.C-Tg(CMV-Cre)1Cgn/J(#006054) mice were obtained from the Jackson Laboratory. HepaCAM knock-out (KO) mice were generated by breeding HepaCAM floxed mice (a kind gift from Dr. Cagla Eroglu at Duke University)^{19 (link)} with CMV-Cre mice. Both male and female mice were used in all experiments. Both sexes were used in all studies. All mice were maintained on a 12 h light/dark cycle with food and water ad libitum.