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Anti ovalbumin ige mouse elisa kit

Manufactured by Cayman Chemical
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The Anti-Ovalbumin IgE (mouse) ELISA kit is a laboratory tool used for the quantitative detection of anti-ovalbumin IgE antibodies in mouse samples. The kit utilizes the enzyme-linked immunosorbent assay (ELISA) technique to measure the levels of these specific antibodies.

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Lab products found in correlation

Anti ovalbumin igg1 mouse elisa kit, by Cayman Chemical (1 mentions) Mouse ifn γ elisa kit, by Solarbio (1 mentions) Mouse il 4 elisa kit, by Solarbio (1 mentions) Bio plex suspension array system, by Bio-Rad (1 mentions) Ensight multimode plate reader, by PerkinElmer (1 mentions) Bio plex pro mouse cytokine assay, by Bio-Rad (1 mentions)

4 protocols using anti ovalbumin ige mouse elisa kit

1

Ovalbumin-Induced Allergy Biomarkers

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NALF was centrifugalized to collect the supernatant. The blood was collected from the vena cava, and then serum was collected via centrifugation. Nasal tissues of sacrificed mice were stored at −70°C, and then homogenated and centrifuged to collect the supernatant. The anti-OVA Ig (immunoglobulin) E, anti-OVA IgG1 and anti-OVA IgG1 levels in serum, and IFN-γ, IL-12, IL-4, IL-5, and IL-13 levels in the NALF and nasal mucosa were measured through enzyme-linked immunosorbent assays (ELISA) using an anti-ovalbumin IgE (mouse) ELISA kit (500840, Cayman, MI, USA), anti-ovalbumin IgG1 (mouse) ELISA kit (500830, Cayman, MI, USA), mouse anti-ovalbumin (Gal d 2) IgG2a ELISA Kit (600-120-02A, Biolead, China), mouse IFN-γ ELISA kit (SEKM-0031, Solarbio, China), mouse IL-12p40 ELISA kit (SEKM-0012, Solarbio, China), mouse IL-13 ELISA kit (SEKM-0014, Solarbio, China), mouse IL-5 ELISA kit (SEKM-0006, Solarbio, China), and mouse IL-4 ELISA kit (SEKM-0005, Solarbio, China) according to the manufacturers’ instructions.
Dong F., Tan J, & Zheng Y. (2020). Chlorogenic Acid Alleviates Allergic Inflammatory Responses Through Regulating Th1/Th2 Balance in Ovalbumin-Induced Allergic Rhinitis Mice. Medical Science Monitor : International Medical Journal of Experimental and Clinical Research, 26, e923358-1-e923358-10.
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2

OVA-Induced Allergic Airway Responses

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Mice were sensitized with 20 μg of OVA emulsified with 2 mg of Imject Alum Adjuvant in 150 μl PBS by i.p. injection. 21 days after OVA sensitization, mice were anesthetized and challenged with 500ug of OVA in 50 μl of PBS and the airway mechanics were measured using the Flexivent system. The airway resistance increased gradually after the OVA injection, reaching the peak at 50-60 seconds after the injection. To examine the level of OVA-specific IgE, serum of sensitized mice was collected on day 18 (before challenge). The OVA-specific IgE level was determined using the anti-Ovalbumin IgE (mouse) ELISA kit (CaymanChemical, 500840) following the user’s instructions.
Han L., Limjunyawong N., Ru F., Li Z., Hall O.J., Steele H., Zhu Y., Wilson J., Mitzner W., Kollarik M., Undem B.J., Canning B.J, & Dong X. (2018). Mrgprs on vagal sensory neurons contribute to bronchoconstriction and airway hyperresponsiveness. Nature neuroscience, 21(3), 324-328.
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3

OVA-Induced Allergic Airway Responses

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Mice were sensitized with 20 μg of OVA emulsified with 2 mg of Imject Alum Adjuvant in 150 μl PBS by i.p. injection. 21 days after OVA sensitization, mice were anesthetized and challenged with 500ug of OVA in 50 μl of PBS and the airway mechanics were measured using the Flexivent system. The airway resistance increased gradually after the OVA injection, reaching the peak at 50-60 seconds after the injection. To examine the level of OVA-specific IgE, serum of sensitized mice was collected on day 18 (before challenge). The OVA-specific IgE level was determined using the anti-Ovalbumin IgE (mouse) ELISA kit (CaymanChemical, 500840) following the user’s instructions.
Han L., Limjunyawong N., Ru F., Li Z., Hall O.J., Steele H., Zhu Y., Wilson J., Mitzner W., Kollarik M., Undem B.J., Canning B.J, & Dong X. (2018). Mrgprs on vagal sensory neurons contribute to bronchoconstriction and airway hyperresponsiveness. Nature neuroscience, 21(3), 324-328.
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4

Measuring Allergy-Related Biomarkers in Mice

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Serum and nasal lavage fluid (NLF) were obtained from each mouse. The concentrations of OVA-sIgE in serum were detected using the Anti-Ovalbumin IgE (mouse) ELISA Kit (Cayman Chemical, Ann Arbor, MI, USA) and an EnSight Multimode Plate Reader (PerkinElmer, Waltham, MS, USA). The concentrations of IFN-γ, IL-4, IL-5, IL-13, IL-10, and IL-17A were detected using the Bio-Plex suspension array system (Bio-Rad) according to the Bio-Plex Pro Mouse Cytokine Assays Quick Guide.
Tan L., Fu L., Zheng L., Fan W., Tan H., Tao Z, & Xu Y. (2022). TET2 Regulates 5-Hydroxymethylcytosine Signature and CD4+ T-Cell Balance in Allergic Rhinitis. Allergy, Asthma & Immunology Research, 14(2), 254-272.
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