Trypto casein soy agar

Manufactured by Solabia

Sourced in Spain

Trypto-Casein Soy agar is a laboratory culture medium used for the cultivation and enumeration of microorganisms. It provides a nutritious environment for the growth of a variety of bacterial species.

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Lab products found in correlation

Doxorubicin, by Teva Pharmaceuticals (2 mentions) Mh agar plates, by Solabia (2 mentions) Yeast extract, by Merck Group (1 mentions) Bacteriological agar, by Molar Chemicals (1 mentions) Mgso4 7h2o, by Reanal (1 mentions) Carbonyl cyanide 3 chlorophenylhydrazone, by Merck Group (1 mentions) Sodium chloride (nacl), by Molar Chemicals (1 mentions) Mhb 2, by Merck Group (1 mentions) Reserpine, by Merck Group (1 mentions) 3 n morpholino propanesulfonic acid mops, by Merck Group (1 mentions) Crystal violet, by Merck Group (1 mentions) Dimethyl sulfoxide (dmso), by Merck Group (1 mentions) Sabouraud dextrose agar, by bioMérieux (1 mentions) Vancomycin van, by Thermo Fisher Scientific (1 mentions) Rpmi 1640 broth medium, by Harvard Bioscience (1 mentions) Cefotaxime ctx, by Duchefa Biochemie (1 mentions) Antibiotic disks, by Liofilchem (1 mentions)

5 protocols using trypto casein soy agar

Antimicrobial Compound Screening Media

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In the experiments described herein, the culture media used were the following: cation-adjusted Mueller–Hinton broth (MHB II; Sigma-Aldrich, St. Louis, MO, USA and Biokar Diagnostics, Allone, Beauvais, France), Luria–Bertani broth (LB-B; Sigma, St. Louis, MO, USA), Tryptic Soy broth (TSB; Scharlau Chemie S. A., Barcelona, Spain), and Trypto-Casein Soy agar (TSA; Biokar Diagnostics, Allone, Beauvais, France). For the QS inhibition assays, the agar medium used was Luria–Bertani with a few modifications (LB*-A) and was prepared in house. The composition was as follows: 1.0 g yeast extract (Merck, Darmstadt, Germany), 10.0 g tryptone (Biolab, Budapest, Hungary), 10.0 g NaCl (Molar Chemicals, Halásztelek, Hungary), 1.0 g K₂HPO₄ (Biolab, Budapest, Hungary), 0.3 g MgSO₄·7 H₂O (Reanal, Budapest, Hungary), 5 mL Fe-EDTA stock solution and 20.0 g of bacteriological agar (Molar Chemicals, Halásztelek, Hungary) per liter of media.
The chemicals used—DMSO, 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT), sodium dodecyl sulfate (SDS), phosphate-buffered saline (PBS; pH 7.4), ethidium bromide (EB), reserpine, carbonyl cyanide 3-chlorophenylhydrazone (CCCP), PMZ, PAβN, and CV—were bought from Sigma-Aldrich Chemie GmbH (Steinheim, Germany), and doxorubicin (2 mg/mL) was purchased from Teva Pharmaceuticals, Budapest, Hungary.

Resende D.I., Durães F., Zubarioglu S., Freitas-Silva J., Szemerédi N., Pinto M., Pinto E., Martins da Costa P., Spengler G, & Sousa E. (2024). Antibacterial Potential of Symmetrical Twin-Drug 3,6-Diaminoxanthones. Pharmaceuticals, 17(2), 209.

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Culturing and Characterizing Microorganisms

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The culture media used in the experiments were the following: cation-adjusted Mueller-Hinton broth (MHB II; Sigma-Aldrich, St- Louis, MO, USA and Biokar Diagnostics, Allone, Beauvais, France), Tryptic Soy broth (TSB; Scharlau Chemie S. A., Barcelona, Spain), and Trypto-Casein Soy agar (TSA; Biokar Diagnostics) were purchased. Sabouraud Dextrose Agar (SDA) was purchased from Bio-Mérieux (Marcy L’Etoile, France), RPMI-1640 broth medium from Biochrom AG (Berlin, Germany), which was buffered with 3-(N-morpholino) propanesulfonic acid (MOPS), purchased from Sigma-Aldrich, to pH 7.0.
Dimethyl sulfoxide (DMSO), 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT), sodium dodecyl sulfate (SDS), phosphate-buffered saline (PBS; pH 7.4), ethidium bromide (EB), reserpine, and crystal violet (CV) were purchased from Sigma-Aldrich Chemie GmbH (Steinheim, Germany). Doxorubicin 2 mg/mL was purchased from Teva Pharmaceuticals, Budapest, Hungary. The antibiotics cefotaxime (CTX) was purchased from Duchefa Biochemie, Haarlem, The Netherlands, and vancomycin (VAN) from Oxoid, Basingstoke, England.

Jesus A., Durães F., Szemerédi N., Freitas-Silva J., da Costa P.M., Pinto E., Pinto M., Spengler G., Sousa E, & Cidade H. (2022). BDDE-Inspired Chalcone Derivatives to Fight Bacterial and Fungal Infections. Marine Drugs, 20(5), 315.

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Bacterial Growth Media Comparison

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Two general-purpose media were used to grow the 3 bacterial species, Brain-Hearth Infusion Broth^™ and Trypto-Casein Soy Agar^™, both from BIOKAR Diagnostics©, Allonne, France.

Rodrigues P.M., Luís J, & Tavaria F.K. (2022). Image Analysis Semi-Automatic System for Colony-Forming-Unit Counting. Bioengineering, 9(7), 271.

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Antibiotic Susceptibility Profiling of Enterobacteriaceae

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The antibiotic susceptibility profile was evaluated for 44 isolates of Enterobacteriaceae (28 E. coli and 16 Klebsiella spp. isolates) using the disc diffusion method on Mueller–Hinton (MH) agar plates (Biokar Diagnostics, Beauvais, France) with antibiotic disc (Liofilchem, Roseto degli Abruzzi, Italy) according to the Clinical Laboratory Standards Institute (CLSI) [38 ]. Isolated colonies grown on Trypto–Casein–Soy agar (Biokar Diagnostics) for 22 ± 2 h at 37 °C were suspended in sterile saline until the turbidity was equivalent to the Mc Farlands 0.5 standard. The resulting bacterial suspensions were used to inoculate MH plates. After antibiotic disc deposition, the plates were incubated for 18 ± 2 h at 37 °C. Fifteen antibiotics were used: amoxicillin (AMX) 10 mg; amoxicillin/clavulanic acid (AMC) 30:10 mg; ceftazidime (CAZ) 30 mg; imipenem (IPM) 10 mg; cefpirome (CPO) 30 mg; aztreonam (ATM) 30 mg; cefoxitin (FOX) 30 mg; ampicillin (AMP) 10 mg; cefotaxime (CTX) 30 mg; chloramphenicol (CHL) 30 mg; tetracycline (TET) 30 mg; gentamycin (GEN) 10 mg; trimethoprim/sulfamethoxazole (SXT) 1:19 mg; azithromycin (AZM) 15 mg; ciprofloxacin (CIP) 5 mg. In each 90 mm plate, five different antibiotic discs were applied, and two replicates were used for each antibiotic. The ranges of the diameter of each antibiotic disc for analysis according to CLSI [38 ] are in Supplemental Table S1.

Salamandane A., Vila-Boa F., Malfeito-Ferreira M, & Brito L. (2021). High Fecal Contamination and High Levels of Antibiotic-Resistant Enterobacteriaceae in Water Consumed in the City of Maputo, Mozambique. Biology, 10(6), 558.

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Antibiotic Susceptibility Profiling of E. coli

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The antibiotic susceptibility profile was evaluated for 59 isolates of E. coli, using the disk diffusion method on Mueller-Hinton (MH) agar plates (Biokar Diagnostics, Beauvais, France) with antibiotic disks (Liofilchem, Roseto degli Abruzzi, Italy) according to the Clinical Laboratory Standards Institute (CLSI, 2021) [31] . Strain E. coli ATCC 25922 was used as a control. Briefly, isolated colonies grown on Trypto-Casein-Soy agar (Biokar Diagnostics) at 37 • C for 18 ± 2 h were suspended in sterile saline until the turbidity was equivalent to that of the McFarlands 0.5 standard (ca. 106 CFU/mL). The resulting bacterial suspensions were used to inoculate MH plates. After disk deposition (four per plate), plates were incubated for 18 ± 2 h at 37 • C. Fifteen antibiotics were used: amoxicillin (AMX) 10 µg, amoxicillin/clavulanic acid (AMC) 30:10 µg, ceftazidime (CAZ) 30 µg, imipenem (IPM) 10 µg, cefpirome (CPO) 30 µg, aztreonam (ATM) 30 µg, cefoxitin (FOX) 30 µg, ampicillin (AMP) 10 µg, cefotaxime (CTX) 30 µg, chloramphenicol (CHL) 30 µg, tetracycline (TET) 30 µg, gentamycin (GEN) 10 µg, trimethoprim/sulfamethoxazole (SXT) 1:19 µg, azithromycin (AZM) 15 µg, ciprofloxacin (CIP) 5 µg. The ranges of the diameters of the inhibition halos for each antibiotic used according to the CLSI 2021 [31] are listed in the Supplemental Table S1.

Salamandane A., Alves S.B., Chambel L., Malfeito‐Ferreira M., & Brito L. (2022). Characterization of Escherichia coli from Water and Food Sold on the Streets of Maputo: Molecular Typing, Virulence Genes, and Antibiotic Resistance. Applied Microbiology, 2(1), 133-147.

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