Rnu6 2
RNU6-2 is a small nuclear RNA (snRNA) molecule that is a component of the U6 small nuclear ribonucleoprotein (snRNP) complex. The U6 snRNP is involved in the spliceosome, which is responsible for the removal of introns from pre-mRNA during the process of gene expression.
4 protocols using rnu6 2
Quantification of miR-34a Expression
Validation of miRNA Expression in Ovarian Cancer Cells
Quantitative Analysis of miRNA Profiles
Profiling miRNA Expression in Fibrosis
miRNA Real-time PCR Analysis Cells were cultured on 35-mm culture dishes and treated with TGF-b1 (10 ng/mL) for 72 h, or BLM (0.4 mM) and MTX (0.1 mM) for 144 h. miRNA was extracted from the cells with miRNeasy Mini Kit and was reverse transcribed into cDNA using miScript Reverse Transcription Kit. Real-time PCR was performed using miScript SYBR Green PCR Kit, according to the manufacturer's instructions. The PCR conditions were as follows: initial denaturation for 1 cycle of 15 min at 95 C, followed by specified cycles of 15 s at 94 C (denaturation), 30 s at 55 C (annealing), and 30 s at 70 C (extension). A melting curve was obtained after the reaction to confirm the single product. The primers for rno-miR-34a-5p, rno-miR-222-3p, rno-miR-200a-3p, and RNU6-2 were purchased from QIAGEN. The expression level of miRNA was normalized as to that of RNU6-2.
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