Metacarb 87h

Manufactured by Agilent Technologies

Sourced in United States, Japan

Metacarb 87H is a high-performance liquid chromatography (HPLC) column designed for the separation and analysis of organic compounds. The column features a cation exchange resin with a sulfonated polystyrene-divinylbenzene matrix and provides efficient separation performance.

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Lab products found in correlation

L 2400, by Hitachi (9 mentions) L 2200, by Hitachi (9 mentions) Seveneasy, by Mettler Toledo (7 mentions) 1200 series, by Agilent Technologies (1 mentions) Libra s22 spectrophotometer, by Harvard Bioscience (1 mentions) Ankom 220 fiber analyzer, by ANKOM Technology (1 mentions) 1260 infinity 2, by Agilent Technologies (1 mentions) 1220 infinity, by Agilent Technologies (1 mentions) Chrompass software, by Jasco (1 mentions) Lc 2000plus series system, by Jasco (1 mentions) D glucose anhydrous, by Scharlab (1 mentions) Ri 1530, by Jasco (1 mentions) Ri 71, by Merck Group (1 mentions) Toc vcsh, by Shimadzu (1 mentions)

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Metacarb 87H column (27 citations)

22 protocols using metacarb 87h

Rumen Fermentation Analysis Protocol

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A press and sensor machine (EA-6, Laurel Electronics, Costa Mesa, CA, USA) was
used to measure the total gas (TG). The pH was determined using the Schott
^® Instrumets Lab 860 (SI Analytics GmbH, Mainz, Germany)
after opening each serum bottle. Samples of fermentation were also collected in
1.5 mL cryotubes and deep frozen at −80°C. These samples were
later thawed at room temperature and then centrifuged at 13,000×g for 15
min at 4°C using a Micro 17TR centrifuge (Hanil Science Industrial,
Incheon, Korea) and the supernatant was used for ammonia nitrogen
(NH₃-N) and volatile fatty acids (VFA) analysis [22 (link)]. Using the Libra S22 spectrophotometer
(Biochrom, Cambridge, UK) at an absorbance of 630 nm, NH₃-N
concentration was measured according to the methods developed by Chaney and
Marbach [25 (link)]. VFA were analyzed using
high performance liquid chromatography (Agilent Technologies1200 series, Agilent
Technologies, Santa Clara, CA, USA) with a UV detector (210 nm and 220 nm) and a
Metacarb87H (Agilent Technologies) column using 0.0085 N
H₂SO₄ as a buffer at a flow rate of 0.6 mL/min and
temperature column of 35°C. The DM disappearance in the rumen was
calculated according to the protocol of Van Emon et al. [26 (link)]. For NDF and acid detergent fiber (ADF) analysis, ANKOM
220 Fiber Analyzer (AnkomTechnology) based on the Van Soest et al. [20 (link)] method was used.

Son A.R., Kim S.H., Valencia R.A., Jeong C.D., Islam M., Yang C.J, & Lee S.S. (2021). Kimchi cabbage (Brassica rapa L.) by-products treated with calcium oxide and alkaline hydrogen peroxide as feed ingredient for Holstein steers. Journal of Animal Science and Technology, 63(4), 841-853.

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Characterization of Rice Husk Composition

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For the characterization of RHE, high-performance liquid chromatography (HPLC) was performed. For hydrolysis, 0.5 g of the rice husk was weighed (previously ground and sifted) and placed in a flask to which 5 mL of H₂SO₄ at 72% was added. The samples were agitated manually for 1 h at 30 °C. After the agitation of the samples, water was added to dilute the acid to 4%. Post-hydrolysis, the sample content in the flasks was autoclaved for 1 h at 121 °C. After the treatment, the samples were filtered, and the solid part of the samples was dried for 24 h and weighed for the determination of the amount of Klason lignin present in the rice husk. The liquid phase of the sample was filtered through a 0.45 µm nylon filter for the injection of the sample into the HPLC system (Agilent 1260 Infinity II, Ratingen, Germany). The analysis was performed on Metacarb 87H (300 mm × 7.8 mm, Agilent, Ratingen, Germany) at temperature of 60 °C with a 0.7 mL/min flow rate of 0.005 mol/L sulfuric acid as the mobile phase. Xylose, arabinose, glucose, and acetic acid were determined and related to the polysaccharides present in the rice husk [36 (link)].

Cid-Ibarra G., Rodríguez-Jasso R.M., Rosero-Chasoy G., Belmares R., Carlos Contreras-Esquivel J., Machado-Cepeda S., Cabello-Galindo A, & Ruiz H.A. (2024). Microalgae Biomass Production from Rice Husk as Alternative Media Cultivation and Extraction of Phycocyanin Using 3D-Printed Ohmic Heating Reactor. Foods, 13(9), 1421.

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HPLC Quantification of Formic Acid

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Formic acid conversion was calculated using HPLC (high-performance liquid chromatography). Liquid samples of the reaction mixture were withdrawn periodically (0.1 ml), diluted to 10 ml using deionised water and analysed by HPLC, Agilent 1220 Infinity LC using a column Agilent MetaCarb 87H 250 × 4.6 mm at 60 °C equipped with an variable wavelength detector (VWD) at 210 nm. The eluent was an aqueous solution of phosphoric acid (0.1 wt%) and the flow rate was set to 0.4 ml min⁻¹. Succinic acid was used as an external standard for the quantification of formic acid (Fig. S1A).

Sanchez F., Motta D., Roldan A., Hammond C., Villa A, & Dimitratos N. (2018). Hydrogen Generation from Additive-Free Formic Acid Decomposition Under Mild Conditions by Pd/C: Experimental and DFT Studies. Topics in Catalysis, 61(3), 254-266.

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Quantification of Fermentation Byproducts

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Glucose, galactose, fucose, formic acid, acetic acid, HMF, furfural and bioethanol were analyzed via High-Performance Liquid Chromatography (HPLC) using an Agilent 1260 Infinity II Perkin Elmer(USA) equipped with a refractive index detector and a MetaCarb 87H column (300 mm x 7.8 mm, Agilent). The mobile phase was 5 mM H2SO4 set at a flow rate of 0.7 mL/min at 60 °C and the injection volume was 20 µL. Authentic standards of each compound of interest over a concentration range of 0 -10 g/L were employed to generate calibration curves and were used for reference and quantification (Pino et al., 2018) . Prior to HPLC analysis, all samples were filtered through a 0.45 μm nylon filter.

Aparicio E., Rodríguez-Jasso R.M., Pinales-Márquez C.D., Loredo-Treviño A., Robledo-Olivo A., Aguilar C.N., Kostas E.T, & Ruiz H.A. (2021). High-pressure technology for Sargassum spp biomass pretreatment and fractionation in the third generation of bioethanol production. Bioresource technology, 329.

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Silage Quality Evaluation Protocol

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Silage pH and ammonia-N were measured using pH meter (SevenEasy; Mettler Toledo, Greifensee, Switzerland) and the colorimetric method described by Chaney and Marbach [11 (link)], respectively. The silage extraction was centrifuged at 5,645×g for 15 min to separate supernatant from silage residue. Collected supernatant was used for lactate and VFA analyses. The concentrations of lactate and VFA were determined using HPLC (L-2200; Hitachi, Tokyo, Japan) fitted with a UV detector (L-2400; Hitachi, Tokyo, Japan) and a column (Metacarb 87H; Varian, Palo Alto, CA, USA) according to the method described by Muck and Dickerson [12 (link)].

Lee S.S., Paradhipta D.H., Lee H.J., Joo Y.H., Noh H.T., Choi J.S., Ji K.B, & Kim S.C. (2020). Application of lactic acid bacteria producing antifungal substance and carboxylesterase on whole crop rice silage with different dry matter. Animal Bioscience, 34(6), 1029-1037.

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BSG Enzymatic Hydrolysis and Characterization

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After the enzymatic hydrolysis of BSG_EF, total phenol (TP) content was determined by the Folin–Ciocalteau method, using gallic acid as standard, and the results were expressed in mg gallic acid equivalents (GAE)/g BSG_EF. The antioxidant activity (AA) was analyzed by the DPPH method, as described by Fernandes et al. (2019) (link), and the results were expressed in µmol Trolox equivalents (TE)/g BSG_EF. The released reducing sugars (glucose, xylose, and arabinose) were analyzed by high-performance liquid chromatography using a Jasco830-IR intelligent refractive-index detector with a Varian MetaCarb 87H column. The column was eluted with 0.005 M H₂SO_4, and the flux was settled at 0.7 ml min⁻¹ at 60°C. The results for each sugar and total released reducing sugars (sum of glucose, xylose, and arabinose) were expressed as mg g⁻¹ BSG_EF.

Fernandes H., Salgado J.M., Ferreira M., Vršanská M., Fernandes N., Castro C., Oliva-Teles A., Peres H, & Belo I. (2022). Valorization of Brewer’s Spent Grain Using Biological Treatments and its Application in Feeds for European Seabass (Dicentrarchus labrax). Frontiers in Bioengineering and Biotechnology, 10, 732948.

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Silage Extraction and Analysis Protocol

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Twenty grams of silage were mixed along with 200 mL of distilled water to make silage extraction for pH, ammonia-N, lactate, volatile fatty acid (VFA), and microbial counts. The pH was measured with electric pH meter (SevenEasy, Mettler Toledo, Greifensee, Switzerland) and ammonia-N was analyzed by colorimetry [21 (link)]. For lactate and VFA analyses, the silage extraction was centrifuged at 5,645×g for 15 min and collected the supernatant. The concentrations of lactate and VFA were determined using HPLC (L-2200, Hitachi, Tokyo, Japan) fitted with a UV detector (L-2400; Hitachi, Japan) and a column (Metacarb 87H; Varian, Palo Alto, CA, USA) described by Muck and Dickerson method [22 ].

Lee S.S., Lee H.J., Paradhipta D.H., Joo Y.H., Kim S.B., Kim D.H, & Kim S.C. (2018). Temperature and microbial changes of corn silage during aerobic exposure. Asian-Australasian Journal of Animal Sciences, 32(7), 988-995.

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Volatile Fatty Acid Quantification

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The sample was centrifuged at 13,500×g for 20 min, and the supernatant was used to determine the concentrations of VFA in the sample. The HPLC (L-2200, Hitachi, Tokyo, Japan) fitted with a UV detector (L-2400, Hitachi, Japan) and a silica gel column (Metacarb 87H, Varian, CA, USA) was used to measure VFA contents as described by Muck and Dickerson (1988) .

Heo W., Kim E.T., Cho S.D., Kim J.H., Kwon S.M., Jeong H.Y., Ki K.S., Yoon H.B., Ahn Y.D., Lee S.S, & Kim Y.J. (2016). The In vitro Effects of Nano-encapsulated Conjugated Linoleic Acid on Stability of Conjugated Linoleic Acid and Fermentation Profiles in the Rumen. Asian-Australasian Journal of Animal Sciences, 29(3), 365-371.

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Determining Glucose Utilization for Lactic Acid Production

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To determine whether the three isolates use glucose as a carbon source for conversion to lactic acid, the isolates were cultured in MRS broth containing 2% glucose at 28°C for 2 days. The bacterial cultures were centrifuged and the supernatant were collected. Each supernatant was filtered through a 0.45-μm cellulose acetate filter for high-performance liquid chromatography (HPLC) analysis. The concentrations of organic acids, such as lactic acid and acetic acid, were determined through HPLC (L-2200; Hitachi, Tokyo, Japan) using an ultraviolet detector (L-2400; Hitachi) and a column (Metacarb 87H; Varian, Palo Alto, CA, USA). HPLC profiles of the culture filtrates were analyzed through comparison with the elution profiles of standard organic acids injected separately.

Choi O., Lee Y., Kang B., Cho S.K., Kang Y., Kang D.W., Lee S.B., Bae S.M, & Kim J. (2023). Identification and characterization of gut-associated lactic acid bacteria isolated from the bean bug, Riptortus pedestris (Hemiptera: Alydidae). PLOS ONE, 18(3), e0281121.

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Silage Quality Analysis Protocols

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Silage pH and ammonia-N were measured using pH meter (SevenEasy, Mettler Toledo, Greifensee, Switzerland) and the colorimetric method described by Chaney and Marbach [17 (link)], respectively. The silage extraction was centrifuged at 5,645×g for 15 min and collected the supernatant for lactate and VFA analyses. The concentrations of lactate and VFA were determined using HPLC (L-2200, Hitachi, Tokyo, Japan) fitted with a UV detector (L-2400; Hitachi, Japan) and a column (Metacarb 87H; Varian, Palo Alto, CA, USA) according to the method described by Muck and Dickerson [18 (link)].

Paradhipta D.H., Joo Y.H., Lee H.J., Lee S.S., Kwak Y.S., Han O.K., Kim D.H, & Kim S.C. (2019). Effects of wild or mutated inoculants on rye silage and its rumen fermentation indices. Asian-Australasian Journal of Animal Sciences, 33(6), 949-956.

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