Human lung fibroblasts or MPCs were added to flow cytometry wash/staining buffer and blocked with anti-human Fc receptor antibodies (BioLegend) for 15 minutes at 4°C. After blocking, cells were stained with anti-human CCR10, CD3, CD4, CD8, CD19, CD68, EpCAM, CD45, EphA3, SSEA4, and PDGFRα for 20 minutes at 4°C. Cells were then washed twice with flow cytometry wash/staining buffer and fixed in 5% neutral buffered formalin (NBF). Representative gating and respective controls of main stainings are summarized in Supplemental Figure 12 .
Anti human fc receptor antibodies
Manufactured by BioLegend
Anti-human Fc receptor antibodies are a type of laboratory equipment used to detect and study Fc receptors on human cells. These antibodies can bind to and label Fc receptors, which are proteins that recognize the Fc portion of antibodies. This allows researchers to identify and analyze cells expressing Fc receptors using techniques such as flow cytometry.
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Lab products found in correlation
3 protocols using anti human fc receptor antibodies
1
Comprehensive Immune Cell Profiling
2
Human Lung Cell Immunophenotyping
3
Human Lung Cell Immunophenotyping
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Human lung cells were added to flow cytometry wash/staining buffer and blocked with anti-human Fc receptor antibodies (Biolegend) for 15 min at 4 °C. After blocking, cells were stained with anti-human CD45, EpCAM, CD3, CD8, CD4, CD45RO, CD45RA, CD28, CTLA4, PD-1 and/or PD-L1 antibodies (Biolegend) for 20 minutes at 4 °C. Unstained, isotype and fluorescent minus one controls (Figure S8 ) were utilized to gate out any non-specific antibody binding and background fluorescence. Cells were then washed twice with flow cytometry wash/staining buffer, fixed in 5% neutral buffered formalin. Flow cytometric data were acquired using a MACSQuant 10 (Miltenyi Biotech) flow cytometer and data were analyzed using Flowjo software V10.2 (Treestar Inc.).
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