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6 protocols using cuprous chloride

1

Synthesis of Copper-Silicon Composite

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Methanol (99.5%), cuprous chloride (97%), cuprous oxide (95%), copper oxide (99%), and bulk metallic copper powder (150 μm) were of reagent grade and were purchased from Sinopharm Chemical Reagent Co., Ltd. Silicon powder (>99.5%) was supplied by Jiangsu Hongda New Materials Co., Ltd. The materials were used as received without further purification.
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2

Dual-Responsive Nanoparticle Synthesis

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MEO2MA, OEGMA, bis[2-(2′-broMoisobutyryloxy)-ethyl]disulfide (BiBOEDS), silver trifluoroacetate (CF3COOAg) and poly(vinylpyrrolidone) (PVP, Mw=55kD) were obtained from Sigma-Aldrich Corp (St Louis, MO, USA). Tris(2-dimethyl-aminoethyl)amine (Me6TREN) and anhydrous sodium hydrosulfide (NaHS) were purchased from Alfa Aesar (Shanghai, China). Ethylene glycol (EG, purity>99.0%), cuprous chloride (CuCl), chloroauric acid hydrate (HAuCl4·4H2O, Au content>47.8%) and 4'6-diamidino-2-phenylindole (DAPI) were purchased from Sinopharm Chemical Reagent Co., Ltd (Beijing, China). DMEM, RPMI 1640 and fetal bovine serum (FBS) were obtained from Gibco BRL/Life Technologies (Grand Island, NY, USA). pHLIP (ACEQNPIYWARYADWLFTTPLLLLDLALLVDADEGT) was synthesized by Bankpeptide Inc. (Heifei, China). All other reagents were of analytical grade and used without any further purification.
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3

ATRP Initiator-Functionalized Silica Nanoparticles

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4-Vinylpyridine, acryl alcohol, and bromoisobutyryl bromide were purchased from Sigma-Aldrich. N,N-Dimethylformamide (DMF), triethylamine, and cuprous chloride (CuCl) were received from Sinopharm Chemical Reagent. Dichloromethane, tris[2-(dimethylamino)ethyl]amine (Me6TREN), and dimethylchlorosilane were purchased from Alfa Aesar. Silica nanoparticles (30 nm, 99.5%) were purchased and used without further purification. Deionized water (18.2 MΩ cm) was obtained through an ultrapure water generator (Ulup, China). Toluene was distilled over sodium using benzophenone as an indicator and stored with molecular sieves. The chlorosilane-functionalized initiator molecules and the initiator-modified silica nanoparticles for ATRP were synthesized according to the literature.27 (link)
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4

Antioxidant and Anti-inflammatory Assays

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Methanol, cordycepin, 97% luminol, 2,2-diphenyl-1-picrylhydrazyl (DPPH) and phorbol 12-myristate 13- acetate (PMA) were purchased from Sigma (USA). Phenol, concentrated sulfuric acid, ethanol, trifluoroacetic acid, cuprous chloride, sodium dihydrogen phosphate, and disodium hydrogen phosphate were purchased from Sinopharm Chemical Reagent Co. Ltd (CN). NKA-II macroporous adsorption resin was purchased from Shanghai Yuanye Biotechnology Co., Ltd (CN). D101 macroporous adsorption resin was purchased from Cangzhou Baoen Adsorbent Material Technology Co. Ltd (CN). 30% hydrogen peroxide was from Shanghai Taopu Chemical Company, and o-phenanthroline was from Shanghai Jingdian Chemical Technology Co. Ltd (CN). AlamarBlue reagent was purchased from Thermo Scientific (USA). All the reagents were of analytical grade.
Mouse macrophage RAW 264.7 and immortalized human keratinocytes (HaCaT cells) were provided by the Institute of Edible Mushrooms, Shanghai Academy of Agricultural Sciences. Malassezia furfur ATCC 14521 was purchased from the China Industrial Microbial Strain Collection Management Center. Dulbecco’s modified Eagle’s medium (DMEM), high glucose DMEM, heat-inactivated fetal bovine serum, glutamine, penicillin, and streptomycin were purchased from Gibco (USA).
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5

Resorcinol-Formaldehyde Synthesis Protocol

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Resorcinol
(≥99.5%,
AR), formaldehyde aqueous solution (37–40%, AR), sodium carbonate
anhydrous (≥99.8%, AR), alcohol (≥99.7%, AR), hydrochloric
acid (36–38%, AR), and cuprous chloride (≥97%, AR) were
purchased from Sinopharm Chemical Reagent Co., Ltd. (Shanghai, China).
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6

Synthesis of Copper and Indium Compounds

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Cuprous chloride (CuCl), indium chloride tetrahydrate (InCl3·4H2O), indium nitrate pentahydrate (In(NO3)3·5H2O), urea (CO(NH2)2), N,N-dimethylformamide, thioacetamide (C2H5NS), anhydrous ethanol, ammonium oxalate (AO), dimethyl sulfoxide (DMSO), isopropyl alcohol (IPA), and benzoquinone (BQ) were obtained from Sinopharm Chemical Reagent Co., Ltd. (China). The lomefloxacin (LOM) was purchased from Aladdin Chemistry Co., Ltd (China). Besides, the potassium dichromate (K2Cr2O7) was supplied by Shiyi Chemical Co., Ltd. (China). All chemicals and reagents are of analytical grade and used directly in the experiment.
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