Alexafluor conjugated and hrp conjugated secondary antibodies

The antibody against BHRF1 was a gift from Jaap Middeldorp (VU University Medical Centre, The Netherlands). Antibodies against BZLF1 and BRLF1 were purchased from Argene (bioMérieux SA, Marcy l'Etoile, France). The rabbit monoclonal antibodies against phospho‐ATM (ab81292), ATM (ab32420), and the anti‐HA tag (1:5000 dilution; ab9110) rabbit polyclonal antibody were purchased from Abcam. The anti‐γ‐H2AX^ser139 antibody was purchased from EMD Millipore (Quincy, MA, USA). The antibodies against CHK2 (#3440) and phospho‐CHK2 (#2197) were purchased from Cell Signaling Technology (Danvers, MA, USA). All of the AlexaFluor‐conjugated and HRP‐conjugated secondary antibodies were purchased from Molecular Probes (New York, NY, USA). The HRP‐conjugated secondary antibodies were purchased from Santa Cruz Biotechnology (Santa Cruz, CA, USA). Western blot analysis was performed as previously described 24 and all the primary and the secondary antibodies used is 1:1000 and 1:5000 in dilutions unless otherwise specified. The signal intensity was measured by ImageJ software (http://rsb.info.nih.gov/ij).

The antibodies for BRCA1 (OP92), γ‐H2AX (05‐636) and Vinculin (MAB3574) were purchased from Millipore (Quincy, MA, USA). The antibodies for RAD51 (ab63801), p53 (ab31333) and ATM (ab32420) were purchased from Abcam (Cambridge, MA, USA). Anti‐p21 (#2974), anti‐p‐CHK1 (S345) (#2348) and anti‐PARP1 (#9542) were obtained from Cell Signaling Technology (Danvers, MA, USA). All of the Alexa Fluor‐conjugated and HRP‐conjugated secondary antibodies were purchased from Molecular Probes (New York, NY, USA). Western blot analysis was performed as previously described,¹³, ⁴⁷ and the signal intensity was measured by ImageJ software (http://rsb.info.nih.gov/ij/).