BMDCs prepared as described earlier were harvested and stained with PerCP/Cy5.5 anti-mouse CD11c, PE anti-mouse CD80, APC anti-mouse I-A/I-E (MHC II), FITC anti-mouse F4/80, or the corresponding isotype control (BioLegend) for 25 min at 4 °C. After washing, cells were analyzed with flow cytometry. Data analysis was performed by using FlowJo software (Tree Star, Ashland, OR, USA), and the results were reported as mean fluorescence intensity.
Apc anti mouse 1 a 1 e
Manufactured by BioLegend
Sourced in United States
APC anti-mouse I-A/I-E is a fluorescent-labeled antibody that binds to the I-A and I-E molecules expressed on the surface of mouse cells. It is a useful tool for the identification and analysis of major histocompatibility complex (MHC) class II-positive cells in mouse samples by flow cytometry.
Automatically generated - may contain errors
2 protocols using apc anti mouse 1 a 1 e
1
Phenotypic Analysis of BMDCs
2
Spleen Cell Phenotypic Analysis
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On day 45, spleen single-cell suspensions were collected from the five groups of mice, filtered with a cell strainer, and stained with PerCP/Cy5.5 anti-mouse CD11c, PE anti-mouse CD80, APC anti-mouse I-A/I-E (major histocompatibility complex II, or MHC II), or the corresponding isotype control (BioLegend, San Diego, CA, USA) for 25 min at 4 °C. After washing with wash buffer, cells were analyzed by using flow cytometry.
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