The heads of cicada specimens were removed from the body with fine needles under a stereomicroscope (Olympus SZX10, Japan), and then rinsed twice for half a minute with 70% ethanol using an ultrasonic cleaner. Samples were then dehydrated in a graded series of 75%, 80%, 85%, 90%, 95% ethanol for 20 min each and 99.9% ethanol for 30 min twice before being transferred to a mixed solution of ethanol and tert-butanol (3:1, 1:1, and 1:3, by volume) for 15 min each, and finally to 100% tert-butanol for 30 min. Then the samples were placed into a freeze-drier (VFD-21S, SHINKKU VD, Japan) for 3 h. The dried sections of mouthparts were then mounted on the aluminum stubs with double-sided copper sticky tape and coated with gold/palladium (40/60) in a high resolution sputter coater (MSP-1S, Hitachi, Tokyo, Japan). The samples were subsequently examined with a Hitachi S-3400N SEM (Hitachi, Tokyo, Japan) operated at 15 kV. Ten individuals of each nymphal instar, and of male and female adults, were observed.
Msp 1s
Manufactured by Hitachi
Sourced in Japan
The MSP-1S is a laboratory equipment manufactured by Hitachi. It is a micro-slicer designed for sectioning small samples with high precision. The MSP-1S can produce thin sections with a thickness range of 0.5 to 20 micrometers.
Automatically generated - may contain errors
Lab products found in correlation
Szx10, by Olympus (2 mentions)
S 3400n sem, by Hitachi (1 mentions)
Tristar 2 3020, by Micromeritics (1 mentions)
Jem 2010, by JEOL (1 mentions)
Iraffinity 1, by Shimadzu (1 mentions)
Tm 1000, by Hitachi (1 mentions)
Jcm 6000, by JEOL (1 mentions)
Nova nano 450 sem, by Thermo Fisher Scientific (1 mentions)
5 protocols using msp 1s
1
SEM Analysis of Cicada Mouthparts
2
Characterization of HA Hollow Microspheres
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The crystalline phase of the HA hollow microspheres was identified by X-ray powder diffraction (XRD) with the CuKα irradiation of an X-ray diffractometer (RINT 2500HR/PC, Rigaku). Fourier transform IR (FTIR) absorption spectroscopy was performed using the KBr method (IRAffinity-1S, Shimadzu). The specific surface area of the microspheres was measured with a surface area and porosity analyzer (Tristar II 3020, Micromeritics). The morphology of the microspheres was observed mainly by scanning electron microscopy (SEM; JCM-6000, JEOL, and TM-1000, Hitachi) after sputter coating with Au-Pd (MSP-1S, Vacuum Device). The wet filtration cakes of the HA microspheres in the PLLA membrane fractured after being frozen in liquid nitrogen to observe the internal structure of the microspheres. When measuring the size of the microspheres a drop of suspension in methanol was placed on a glass coverslip, and the dispersion medium was evaporated at room temperature. Then, the microspheres and coverslip were coated with Au-Pd. Transmittance electron microscopy (TEM) images of the microspheres on a micro grid (STEM 150 Cu Grid with carbon film, Okenshoji) were taken at 200 kV with JEM-2010 (JEOL).
3
Scanning Electron Microscopy of Callitettix versicolor Antennae
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All Callitettix versicolor (Fabricius) specimens used in this study were collected in the Guizhou Province, China and preserved in 70% ethanol and stored at 4 °C.
Heads with antennae were separated from the rest of the body and rinsed in 70% ethanol in an ultrasonic cleaning. Dehydration was achieved with a graded ethanol series of 75%, 80%, 90%, and 95% each for 20 min and twice in 99.9% ethanol solutions for 30 min. The 99.9% ethanol was then substituted successively by mixing alcohol and tert-butyl ethanol with in proportions of 3:1, 1:1 and 1:3. Finally, specimens were placed in pure tert-butyl ethanol for 30 min. Specimens were dried in a freeze-drier (VFD-21S, SHINKKU VD, Tokyo, Japan) for 3 h. Thereafter, the antennae were removed from the dried samples under a stereomicroscope (Olympus SZX10, Olympus, Tokyo, Japan) and placed on aluminum stubs in various positions using double-sided sticky tape. The antennae were sputter-coated with gold-palladium (MSP-1S, Hitachi, Tokyo, Japan) and examined and observed at 15 kV in a Nova nano SEM450. Antennae of male and female adults were observed for comparison.
Heads with antennae were separated from the rest of the body and rinsed in 70% ethanol in an ultrasonic cleaning. Dehydration was achieved with a graded ethanol series of 75%, 80%, 90%, and 95% each for 20 min and twice in 99.9% ethanol solutions for 30 min. The 99.9% ethanol was then substituted successively by mixing alcohol and tert-butyl ethanol with in proportions of 3:1, 1:1 and 1:3. Finally, specimens were placed in pure tert-butyl ethanol for 30 min. Specimens were dried in a freeze-drier (VFD-21S, SHINKKU VD, Tokyo, Japan) for 3 h. Thereafter, the antennae were removed from the dried samples under a stereomicroscope (Olympus SZX10, Olympus, Tokyo, Japan) and placed on aluminum stubs in various positions using double-sided sticky tape. The antennae were sputter-coated with gold-palladium (MSP-1S, Hitachi, Tokyo, Japan) and examined and observed at 15 kV in a Nova nano SEM450. Antennae of male and female adults were observed for comparison.
4
Ultrastructural Analysis of Insect Antennae
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The specimens were rinsed twice with 70% ethanol using an ultrasonic cleaner (KQ118, Kunshan, China), twenty seconds for each time. And then, samples were dehydrated in agraded series of 80%, 90% ethanol for 20 min each and 99.9% ethanol for 30 min twice before being transferred to a mixed solution of ethanol and tertbutanol (3:1, 1:1, and 1:3, by volume) for 15 min each, and finally dipped into 100% tert-butanol for 30 min. After that, the samples were dried with a freeze-drier (VFD-21S, SHINKKUVD, Japan) for 3 h. The dried antennae were then separated from bodies and mounted on the aluminum stubs under a stereomicroscope with double-sided copper sticky tape and coated with gold/palladium (40/60) in a high-resolution sputter coater (MSP-1S, Hitachi, Tokyo, Japan). Fine morphology of four female and four male antennae were examined with a Nova Nano 450 SEM (FEI, America) operated at 15 kV.
5
Scanning Electron Microscopy of Bio-Oss Bone Substitute
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The samples were placed on a conductive object stage, sprayed with gold sputter in a vacuum spray apparatus (MSP-1S; Hitachi, Ltd., Tokyo, Japan) and then scanned by SEM (Hitachi S-3400N; Hitachi, Ltd.) at different magnifications. The surface morphology of Bio-Oss bone substitute in different groups was observed with the electron acceleration voltage set to 10 kV.
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