To exclude effects of angioplasty on monocyte subset distribution and any diurnal variation, blood was drawn in the morning before coronary angiogram. A 21-gauge butterfly needle (0.8 mm × 19 mm; Greiner Bio-One, Kremsmünster, Austria) was used for venipuncture from an antecubital vein. The initial 3 mL of blood were discarded, and blood was drawn into an EDTA tube (Greiner Bio-One) for immediate analysis by flow cytometry. An additional 3.8% sodium citrate Vacuette tube, a serum separator tube and an EDTA tube (all Greiner Bio-One) were collected, centrifuged at 3000 rpm at 4°C for 15 min and stored in aliquots at -80°C for later analysis.
Serum separator tube
Manufactured by Greiner
Sourced in Austria, United States
Serum separator tubes are a type of laboratory equipment used to collect and process blood samples. The primary function of these tubes is to separate the liquid portion (serum) from the cellular components of the blood sample. This separation is achieved through the use of a gel or physical barrier within the tube, which allows for the efficient isolation of the serum for further analysis or testing.
Automatically generated - may contain errors
Lab products found in correlation
Edta tube, by Greiner (4 mentions)
21 gauge butterfly needle, by Greiner (3 mentions)
Sodium citrate vacuette tube, by Greiner (3 mentions)
Vacuette tube, by Greiner (1 mentions)
Zoletil, by Virbac (1 mentions)
Atipamezole, by Pfizer (1 mentions)
Bio plex 3d suspension array system, by Bio-Rad (1 mentions)
Mrx revelation microplate reader, by Dynex (1 mentions)
11 protocols using serum separator tube
1
Blood Draw Protocol for Monocyte Analysis
2
Blood Collection for Coronary Angiography
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
Blood was drawn in the morning prior to elective coronary angiography after venipuncture from an antecubital vein using a 21-gauge butterfly needle (0.8 mm × 19 mm; Greiner Bio-One, Kremsmünster, Austria). After the initial 3 mL of blood were discarded, blood was drawn into an EDTA tube (Greiner Bio-One) for immediate analysis by flow cytometry. Furthermore, a 3.8% sodium citrate Vacuette tube (Greiner Bio-One; nine parts of whole blood, one part of sodium citrate 0.129 M/L), a serum separator tube (Greiner Bio-One) and an EDTA tube (Greiner Bio-One) were collected, immediately centrifuged (4°C; 3000RPM for 15 min) and stored at—80°C for later analysis.
3
Blood Sample Collection for Coronary Angiography
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
Blood was drawn in the morning prior to elective coronary angiography after venipuncture from an antecubital vein using a 21-gauge butterfly needle (0.8 mm × 19 mm; Greiner Bio-One, Kremsmünster, Austria). After the initial 3 mL of blood were discarded, blood was drawn into an EDTA tube (Greiner Bio-One) for immediate analysis by flow cytometry. Furthermore, a 3.8% sodium citrate Vacuette tube (Greiner Bio-One; nine parts of whole blood, one part of sodium citrate 0.129 M/L), a serum separator tube (Greiner Bio-One) and an EDTA tube (Greiner Bio-One) were collected, immediately centrifuged (4 °C; 3000RPM for 15 min) and stored at −80 °C for later analysis.
4
Blood Sampling Protocol for Biomarker Analysis
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
Blood was drawn at the time of admission (24 h time window) from an arterial or central venous line. A serum separator tube, an EDTA‐tube and a 3.8% sodium citrate vacuette tube (Greiner Bio‐One, Austria) were used for collection after discarding the initial 3 mL of blood to ensure stable sampling conditions. Consecutively, samples were centrifuged at 4°C and 3000 RPM for 15 min and stored at −80°C for later analysis. Standard laboratory values including NT‐proBNP were carried out by the department of laboratory medicine of the Vienna general hospital.
5
Induction and Evaluation of Collagen-Induced Arthritis in Mice
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
CIA was induced in 6–10 week old mice as previously described (47 (link)). A total of 100 μL of chick type II collagen (CII, final concentration 1 mg/ml; Sigma, MO, USA) emulsified in complete Freund's adjuvant containing 5 mg/ml heat-killed M. tuberculosis H37RA (BD Difco, NJ, USA) was injected intradermally in two sites at the base of the tail. The injections were repeated 21 days later. Animals were monitored three times weekly for erythema and swelling of limbs, and a clinical score (0–3) was given for each paw. Serum was collected using serum separator tubes (Greiner, Vilvoorde, Belgium) and analyzed for anti-chick collagen type II IgG antibodies by ELISA as described (48 (link)). Standard curves were constructed from pooled sera of CII hyper-immunized DBA/1 mice, set at 100,000 units/ml.
6
Comprehensive Lion Health Assessment
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
Lions were located by wildlife officers' reports, global positioning system satellite locations, or radio telemetry of tagged animals, baiting and calling stations, and by tracking spoor. Samples were collected during winter (June-August) from 13 lions (Table 1), which represents 59% of the 22 adult-subadult lions known to be present in NTGR in 2014. Samples originated from two of the four prides, a coalition of two brothers, and the one lone male in NTGR (Snyman et al. 2014) . Lions were immobilized by dart injection of 0.03-0.05 mg/kg medetomidine 20 mg/mL (Kyron Laboratories, Johannesburg, South Africa) and 0.5-1.0 mg/kg Zoletil 100 mg/mL (tiletaminezolazepam; Virbac, Centurion, South Africa) and reversed with 0.2 mg/kg atipamezole 5 mg/mL (Pfizer, Sandton, South Africa) administered intramuscularly. Once anesthetized, each lion was given a complete physical examination. Blood samples were collected by femoral or cephalic venipuncture and placed into ethylenediaminetetraacetic acid and serum separator tubes (Greiner Bio-one, Monroe, North Carolina, USA). Clotted blood was centrifuged at 1,250 3 G for 15 min and serum was removed and frozen at À20 C until testing. Whole blood was also frozen until testing. The ears and inguinal region were examined for ectoparasites, and representative samples were preserved in 100% ethanol.
7
Serum Preparation from Blood Samples
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
For the biorepository, blood was collected from subjects into serum separator tubes (Greiner Bio-One, Monroe, USA) and centrifuged at 1300x g for 10 minutes. Serum was transferred to a fresh tube and centrifuged at 2000x g for 5 minutes. The supernatant was then aliquoted and stored at -80°C.
8
Blood Sample Collection Protocol
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
On the second experimental day (Figure 1 ), participants visited the laboratory and rested for 30
minutes prior to the first blood sample being taken. Blood samples were drawn
into vacutainers from the antecubital forearm vein using a 20-gauge needle for
the biochemical measurements. Blood was collected into serum-separator tubes
(Greiner Bio-One), allowed to clot for 30 minutes at room temperature, and then
centrifuged at 2000 g for 10 minutes. Sera were then harvested
in aseptic conditions and frozen at −80°C for storage.
minutes prior to the first blood sample being taken. Blood samples were drawn
into vacutainers from the antecubital forearm vein using a 20-gauge needle for
the biochemical measurements. Blood was collected into serum-separator tubes
(Greiner Bio-One), allowed to clot for 30 minutes at room temperature, and then
centrifuged at 2000 g for 10 minutes. Sera were then harvested
in aseptic conditions and frozen at −80°C for storage.
9
Cytokine Profiling of Nanoparticle-Injected Mice
10
Quantification of Serum Granulysin Levels
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
Serum GNLY concentrations were measured using the LEGEND MAX™ Human Granulysin ELISA Kit (cat. no. 438007; BioLegend, Inc.). The sera of OA patients and controls were prepared from peripheral venous blood samples (3 ml/sample), which were taken by venipuncture in plastic Serum Separator Tubes, 3.5 ml (Greiner Bio-One GmbH), allowed to clot for 20 min at 22-25˚C, and centrifuged (1,000 x g, 10 min, at 4˚C). The supernatant (serum) was collected in Cryo.S, 2 ml, round bottom, screw-cap vial (Greiner Bio-One GmbH) and stored at -80˚C until required for ELISA. Assays were performed following the manufacturer's instructions, and the absorbance was measured using an MRX Revelation microplate reader (Dynex Technologies Inc.).
About PubCompare
Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.
We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.
However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.
Ready to get started?
Sign up for free.
Registration takes 20 seconds.
Available from any computer
No download required
Revolutionizing how scientists
search and build protocols!