Transformed Hsp104 variants and controls were grown overnight in raffinose media. The overnight cultures were diluted to an OD600 of 0.3 (A600nm = 0.3) and grown in galactose-supplemented media at 30°C. α-synuclein samples were induced for 8h, while FUS and TDP-43 samples were induced for 5h. Samples were then normalized to an OD600 of 0.6 (A600nm = 0.6). The pelleted cells were resuspended in 0.1 M NaOHfor5 min and then pelleted again and resuspended in 1xSDS sample buffer. The samples were then boiled and separated by SDS-PAGE (4%–20% gradient, Bio-Rad) and transferred to a PVDF membrane. The following primary antibodies were used: anti-GFP polyclonal (Sigma-Aldrich), anti-FUS polyclonal (Bethyl Laboratories), anti-TDP-43 polyclonal (Proteintech), anti-Hsp104 polyclonal (Enzo Life Sciences), and anti-PGK monoclonal (Invitrogen). Two fluorescently labeled secondary antibodies were used: anti-rabbit (Li-Cor) and anti-mouse (Li-Cor). Blots were imaged using a LI-COR Odyssey FC Imaging system.
Anti gfp polyclonal
Manufactured by Merck Group
The Anti-GFP polyclonal antibody is a laboratory reagent used to detect and quantify the presence of green fluorescent protein (GFP) in various experimental systems. It is a polyclonal antibody, meaning it recognizes multiple epitopes on the GFP protein. The antibody can be used in a range of applications, including Western blotting, immunoprecipitation, and immunofluorescence microscopy, to identify and analyze GFP-tagged proteins.
Automatically generated - may contain errors
Lab products found in correlation
Anti tdp 43 polyclonal, by Proteintech (2 mentions)
Anti fus polyclonal, by Fortis Life Sciences (2 mentions)
Anti hsp104 polyclonal, by Enzo Life Sciences (2 mentions)
Anti pgk monoclonal, by Thermo Fisher Scientific (2 mentions)
Odyssey fc imaging system, by LI COR (2 mentions)
Sds page, by Bio-Rad (2 mentions)
2 protocols using anti gfp polyclonal
1
Monitoring Protein Aggregation Dynamics
2
Monitoring Protein Aggregation Dynamics
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Transformed Hsp104 variants and controls were grown overnight in raffinose media. The overnight cultures were diluted to an OD600 of 0.3 (A600nm = 0.3) and grown in galactose-supplemented media at 30°C. α-synuclein samples were induced for 8h, while FUS and TDP-43 samples were induced for 5h. Samples were then normalized to an OD600 of 0.6 (A600nm = 0.6). The pelleted cells were resuspended in 0.1 M NaOHfor5 min and then pelleted again and resuspended in 1xSDS sample buffer. The samples were then boiled and separated by SDS-PAGE (4%–20% gradient, Bio-Rad) and transferred to a PVDF membrane. The following primary antibodies were used: anti-GFP polyclonal (Sigma-Aldrich), anti-FUS polyclonal (Bethyl Laboratories), anti-TDP-43 polyclonal (Proteintech), anti-Hsp104 polyclonal (Enzo Life Sciences), and anti-PGK monoclonal (Invitrogen). Two fluorescently labeled secondary antibodies were used: anti-rabbit (Li-Cor) and anti-mouse (Li-Cor). Blots were imaged using a LI-COR Odyssey FC Imaging system.
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