Fourier-transform infrared (FT-IR) spectra (650–4000 cm−1) were recorded in the transmission mode on a Perkin Elmer FT-IR Spectrum 400 spectrophotometer (Perkin Elmer, Mexico). UV-visible spectra were collected on a Shimadzu spectrophotometer model UV 2700 (Shimadzu, Japan) using a 1 cm path-length quartz cell. Fluorescence spectra were recorded from 300 to 700 nm on a Horiba Nanolog fluorescence spectrophotometer (HORIBA Scientific, Edison, NJ, USA) using a xenon lamp as the excitation source and a 1 cm path-length fluorescence quartz cell. The morphologies and sizes of nanoparticles were characterized by JEM-2200FS (JEOL, Akishima, Japan) transmission electron microscopy (TEM). The microscope was capable of spherical aberration correction in a scanning transmission electron microscope (STEM) mode working at an accelerating voltage of 200 keV. The images were acquired by a high-angle annular dark-field (HAADF) detector. Zeta potential measurements were acquired using a Horiba Scientific SZ-100 nanoparticle analyzer (Horiba Scientific, Japan). A Thermo Scientific pH meter was utilized to measure the pH of the solutions (Thermo Fisher Scientific, Mexico City, Mexico).
Ft ir spectrum 400 spectrophotometer
Manufactured by PerkinElmer
Sourced in Mexico
The FT-IR Spectrum 400 spectrophotometer is a laboratory instrument designed for infrared spectroscopy analysis. It is capable of detecting and measuring the absorption of infrared radiation by molecules, which can provide information about their chemical structure and composition.
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Lab products found in correlation
Jem 2200f, by JEOL (1 mentions)
Sz 100 nanoparticle analyzer, by Horiba (1 mentions)
Nanolog fluorescence spectrophotometer, by Horiba (1 mentions)
Silica gel 60, by GE Healthcare (1 mentions)
Ascend 400 ft nmr spectrometer, by Bruker (1 mentions)
Pre coated silica gel 60, by Merck Group (1 mentions)
J 810 spectropolarimeter, by Jasco (1 mentions)
Uv 1800 spectrophotometer, by Shimadzu (1 mentions)
Microtof q 2 mass spectrometer, by Bruker (1 mentions)
2 protocols using ft ir spectrum 400 spectrophotometer
1
Characterization of Nanomaterials by Spectroscopy
2
Characterization of Natural Compounds
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Optical rotations were measured on a JASCO-1020 polarimeter. Electronic Circular Dichroism (ECD) was recorded on a JASCO J-810 spectropolarimeter. UV spectra were collected on a Shimadzu UV 1800 spectrophotometer. IR spectra were recorded in the ATR mode using a PerkinElmer FT-IR Spectrum 400 spectrophotometer. 1H and 13C NMR spectra were recorded on a Bruker ASCEND 400 FT-NMR spectrometer, operating at 400 MHz (1H) and 100 (13C) MHz. HR-ESI-TOF-MS spectra were measured with a Bruker micrOTOF-QII mass spectrometer. Unless otherwise indicated, column chromatography was carried out using Merck silica gel 60 (particle sizes less than 0.063 mm) and GE Healthcare Sephadex LH-20. For thin-layer chromatography (TLC), Merck pre-coated silica gel 60 F254 plates were used. Spots on TLC were detected under UV light and by spraying with anisaldehyde-H2SO4 reagent followed by heating.
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