Thioflavin t ultrapure grade

Manufactured by Eurogentec

Sourced in United Kingdom

Thioflavin T UltraPure Grade is a fluorescent dye used as a reagent in various analytical and research applications. It is a high-purity, specially formulated version of the Thioflavin T compound.

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Lab products found in correlation

1 2 dimyristoyl sn glycero 3 phospho l serine, by Avanti Polar Lipids (2 mentions) 1 2 dimyristoyl sn glycero 3 phospho l serine sodium salt, by Avanti Polar Lipids (2 mentions) Triton x 100, by Merck Group (1 mentions) Ethanol, by Merck Group (1 mentions) Alexa fluor 568 nhs ester, by Thermo Fisher Scientific (1 mentions) Alexa fluor 647 maleimide, by Thermo Fisher Scientific (1 mentions) Sylgard 184 kit, by Dow (1 mentions) Carbon black nanopowder, by Merck Group (1 mentions)

5 protocols using thioflavin t ultrapure grade

Lipid-based Amyloid Aggregation Assay

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1,2-Dimyristoyl-sn-glycero-3-phospho-l-serine (sodium salt; DMPS) was purchased from Avanti Polar Lipids (Alabama, USA). Sodium phosphate monobasic (NaH₂PO₄, BioPerformance Certified, >99.0%), sodium phosphate dibasic (Na₂HPO₄, ReagentPlus, >99.0%) and sodium azide (NaN₃, ReagentPlus, >99.5%) were purchased from Sigma Aldrich (Poole, UK). Thioflavin T UltraPure Grade (ThT, >95%) was purchased from Eurogentec (Southampton, UK).

van der Wateren I.M., Knowles T.P., Buell A.K., Dobson C.M, & Galvagnion C. (2018). C-terminal truncation of α-synuclein promotes amyloid fibril amplification at physiological pH †Electronic supplementary information (ESI) available: Supplementary methods, supplementary results, supplementary tables, supplementary figures. See DOI: 10.1039/c8sc01109e. Chemical Science, 9(25), 5506-5516.

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Thioflavin T Assay for Amyloid Detection

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All reagents were purchased from Sigma Aldrich, excluding thioflavin T UltraPure Grade (ThT ≥ 95%), which was purchased from Eurogentec Ltd.

Ikenoue T., Aprile F.A., Sormanni P, & Vendruscolo M. (2021). Rationally Designed Bicyclic Peptides Prevent the Conversion of Aβ42 Assemblies Into Fibrillar Structures. Frontiers in Neuroscience, 15, 623097.

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Phospholipid Membrane Characterization Protocol

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1,2-dimyristoyl-sn-glycero-3-phospho-L-serine (sodium salt) (DMPS) was purchased from Avanti Polar Lipids, Inc. Sodium phosphate monobasic (NaH₂PO₄, BioPerformance Certified, ≥ 99.0%), sodium phosphate dibasic (Na₂HPO₄, ReagentPlus^®, ≥ 99.0%), sodium chloride (NaCl, BioXtra ≥ 99.5%), 1,6-diphenylhexa-1,3,5-triene (DPH, 98%), ethanol (EtOH, ≥ 99.8%) and Triton X-100 were purchased from Sigma Aldrich. Thioflavin T UltraPure Grade (ThT, ≥ 95%) was purchased from EUROGENTEC LTD.

Galvagnion C., Buell A.K., Meisl G., Michaels T.C., Vendruscolo M., Knowles T.P, & Dobson C.M. (2015). Lipid vesicles trigger α-synuclein aggregation by stimulating primary nucleation. Nature chemical biology, 11(3), 229-234.

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Lipid-based Biomaterial Preparation

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1,2-Dimyristoyl-sn-glycero-3-phospho-L-serine (sodium salt; DMPS) was purchased from Avanti Polar Lipids (Alabama, USA). Sodium phosphate monobasic (NaH₂PO₄, BioPerformance Certified, ≥99.0%), sodium phosphate dibasic (Na₂HPO₄, ReagentPlus, ≥99.0%), sodium azide (NaN₃, ReagentPlus, ≥99.5%) and carbon black nanopowder were purchased from Sigma Aldrich (Poole, UK). Thioflavin T UltraPure Grade (ThT, ≥95%) was purchased from Eurogentec (Southampton, UK) and polydimethylsiloxane (PDMS) (Sylgard 184 kit) from Dow Corning (Midland, Michigan). Alexa Fluor 568 NHS-ester and Alexa Fluor 647 maleimide were purchased from Life Technologies (Carlsbad, California).

Brown J.W., Buell A.K., Michaels T.C., Meisl G., Carozza J., Flagmeier P., Vendruscolo M., Knowles T.P., Dobson C.M, & Galvagnion C. (2016). β-Synuclein suppresses both the initiation and amplification steps of α-synuclein aggregation via competitive binding to surfaces. Scientific Reports, 6, 36010.

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Thioflavin T-based Amyloid Assay

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Thioflavin T UltraPure Grade (ThT > 95%) was purchased from Eurogentec Ltd (Belgium). Sodium phosphate monobasic (NaH₂PO₄, BioPerformance Certified >99.0%), sodium phosphate dibasic (Na₂HPO₄, ReagentPlus, >99.0%) and sodium azide (NaN₃, ReagentPlus, >99.5%) were purchased from Sigma Aldrich, UK. 1,2-Dimyristoyl-sn-glycero-3-phospho-L-serine, sodium salt (DMPS) was purchased from Avanti Polar Lipids, Inc, USA.

Agerschou E.D., Flagmeier P., Saridaki T., Galvagnion C., Komnig D., Heid L., Prasad V., Shaykhalishahi H., Willbold D., Dobson C.M., Voigt A., Falkenburger B., Hoyer W, & Buell A.K. (2019). An engineered monomer binding-protein for α-synuclein efficiently inhibits the proliferation of amyloid fibrils. eLife, 8, e46112.

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