Scmos microscope camera

FEI Quanta 450 FEG scanning electron microscope (SEM, Dawson, NE) at a scanning voltage of 5 kV and a spot size of 4.0 was applied to capture the images of the optofluidic channel at a tilt angle of 45º.
The cells were stained using immunofluorescence staining method at a floating state.
To stain floating cells, the cells were first resuspended by using 0.25 % trypsin-EDTA in phosphate buffered saline (PBS; Sigma-Aldrich, St. Louis, MO). The cells were fixed with 4 % paraformaldehyde (PFA, Sigma-Aldrich, St. Louis, MO) in phosphate buffered saline (PBS, Sigma-Aldrich, St. Louis, MO) for 10 min and then treated with 0.3% Triton X-100 in PBS for 10 min. We applied 10 % goat serum for 1 hr to avoid non-specific binding of the staining molecules in the next steps. The Lamin-A primary antibody (Abcam, Cambridge, MA) was applied for 1hr. The cytoskeletal actin was stained with Alexa-555 conjugated phalloidin (Life Technologies, Carlsbad, CA) followed by staining the nucleus with 0.1% Hoechst 33342 (Sigma-Aldrich, St. Louis, MO) for 10 min. And the stained images were obtained by using laser confocal microscope (TCS SP8 Confocal Microscope, Leica, Germany). Bright field imaging is conducted by a phase-contrast inverted microscope (TE300, Nikon, Tokyo, Japan) and an sCMOS microscope camera (Zyla, Andor, Belfast, UK) was applied to capture highresolution images (~570 nm/pixel).