S monovette z gel tubes

An Airbus A310 performed a single successful test parabola and 30 regular parabolas in which phases of weightlessness were achieved. This microgravity phase of each parabola was flanked by a pull-up and a pull-out phase at about 1.8 g hypergravity (Figure 1A). Each phase lasted about 20 s. The parabolas were performed in six sets of five parabolas. Each set was separated by steady flight of 5 to 8 min. The steady flight in 1 g between two parabolas lasted for 2 min. The cabin conditions remained stable at all times with a pressure of 830 mbar, cabin luminosity of 800 lux and a temperature of 19–21°C with a humidity of 15%. All participants were at rest throughout the flight.
The participants had no prior experience with parabolic flights. Each participant flew just once, and was equipped with an intravenous cannula, from which the blood samples were obtained during the flight. Blood was collected in S-Monovette Z-Gel tubes (Sarstedt, Germany). Serum was obtained by centrifugation at 2500 g, 4 min, room temperature immediately after arrival of the plane. The decanted serum was stored at -80°C until use.

All biosamples were processed within 1 h of collection. Blood was collected into uncoated S-Monovette^® Z-Gel tubes and S-Monovette® EDTA KE tubes (Sarstedt, Leicester, UK) for serum and plasma isolation, respectively. Blood in Z-Gel tubes was allowed to clot for ≥ 20 min before centrifuging at 1600×g for 10 min at 4 °C. EDTA KE tubes were processed immediately upon receipt; samples were centrifuged at 1600×g for 10 min at 4 °C. 1 mL aliquots of serum and plasma were stored in sterile cryovials at − 80 °C.

Blood was collected into uncoated S-Monovette® Z-Gel tubes (Sarstedt, Leicester, UK) and allowed to clot for 20 min before centrifuging at 1,600 × g for 15 min at 4 °C. One-millilitre aliquots of serum were stored in sterile cryovials at − 80 °C.