Lungs were homogenized with ground glass in 2 ml of MEM media by grinding with a mortar and pestle. After centrifugation at 2000×g for 15 min, the supernatant was collected to detect proteins. DuoSet ELISA kits (KC, LIX, TNF-α, IL-1α, IL-1β, IL-6, IL-10, and amphiregulin (AREG)) from R&D Inc. (Minneapolis, MN) and Mouse Myeloperoxidase ELISA Kit from Thermo Fisher Scientific were used to measure the concentration of cytokines, chemokines, and myeloperoxidase in lung homogenates according to manufacturer’s instructions. Values below the detection limit were assigned a half of the lower limit of detection value. Total protein levels in the BAL fluid were measured by Pierce BCA Protein Assay Kit (Thermo Fisher Scientific).
Mouse myeloperoxidase elisa kit
Manufactured by Thermo Fisher Scientific
Sourced in United States
The Mouse Myeloperoxidase ELISA Kit is a quantitative sandwich enzyme-linked immunosorbent assay (ELISA) designed for the measurement of mouse myeloperoxidase (MPO) levels in biological samples.
Automatically generated - may contain errors
Lab products found in correlation
3 protocols using mouse myeloperoxidase elisa kit
1
Cytokine and Chemokine Profiling in Lungs
2
Plasma MPO Quantification by ELISA
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
We obtained plasma by centrifugation at 1500 g for 10 min at 4 °C. Plasma MPO levels were measured using a Mouse Myeloperoxidase ELISA kit (Thermo Fisher Scientific, Waltham, MA, USA) according to the manufacturer's instructions.
3
Quantifying Serum Myeloperoxidase in Mice
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
Mice serum MPO content was detected with an Elisa kit (Thermo, Mouse Myeloperoxidase ELISA Kit). All reagents and samples were brought to room temperature before use, and then 100 µL of each standard and sample were added to the appropriate wells and incubated for 2.5 h at room temperature. The solution was discarded and washed four times with Wash Buffer. Then, 100 µL of prepared biotinylated antibody (diluted 80-fold with Diluent Buffer) were added to each well. The samples were incubated for 1 h at room temperature with gentle shaking. After washing four times with Wash Buffer, the solution was discarded, and the 100 µL of prepared Streptavidin-HRP solution (diluted 400-fold with Diluent Buffer) were added to each well. The samples were incubated for 45 min at room temperature with gentle shaking. Next, after washing four times with Wash Buffer, 100 µL of TMB Substrate were added to each well. The samples were incubated for 30 min at room temperature in the dark with gentle shaking, and then measurements were recorded on a Microplate Reader at 450 nm.
About PubCompare
Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.
We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.
However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.
Ready to get started?
Sign up for free.
Registration takes 20 seconds.
Available from any computer
No download required
Revolutionizing how scientists
search and build protocols!