Cell invasion assays were performed using conventional Boyden transwell methods in keeping with the manufacturer’s protocol (BD BioCoat™ FluoroBlok Invasion System No. 354166). Melanoma cell migration was studied with the use of FluoroBlok Inserts System No. 351157. Quantitation of invasion was achieved by post-invasion cell labelling with Calcein AM (Fluka), and measuring the fluorescence of invading cell samples at excitation/emission wavelengths of 485/530 nm sensitivity 100 on a plate reader (BIO-TEK).
Biocoat fluoroblok invasion system no 354166
Manufactured by BD
The BD BioCoat™ FluoroBlok Invasion System No. 354166 is a cell culture insert system designed for the analysis of cell invasion. The system utilizes a FluoroBlok membrane that blocks the passage of fluorescent labels, allowing for the detection and quantification of invading cells.
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2 protocols using biocoat fluoroblok invasion system no 354166
1
Melanoma Cell Invasion Quantification
2
Boyden Transwell Cell Invasion Assay
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Cell invasion assays were performed using conventional Boyden transwell methods in keeping with the manufacturer’s protocol (BD BioCoat™ FluoroBlok Invasion System No. 354166) described previously [7 (link)].
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