Apoptosis was evaluated by citofluorimetric analysis of Annexin-V and 7-amino-actinomycin-D (7-AAD)-positive cells using the fluorescein isothiocyanate (FITC)-Annexin-V/7-AAD kit (Beckman Coulter, Milan, Italy). Stained cells were analyzed using the FACSCalibur™ (Becton–Dickinson). Positive staining for Annexin-V as well as double staining for Annexin-V and 7-AAD were interpreted as signs of early and late phases of apoptosis respectively [18 (link)].
Fitc annexin v 7 aad kit
Manufactured by Beckman Coulter
Sourced in Italy
The (FITC)-Annexin-V/7-AAD kit is a laboratory tool used for the detection and quantification of apoptosis in cell samples. It contains two fluorescent dyes: FITC-conjugated Annexin-V, which binds to phosphatidylserine, and 7-AAD, which stains DNA. This kit allows researchers to distinguish between viable, early apoptotic, and late apoptotic/necrotic cells through flow cytometric analysis.
Automatically generated - may contain errors
Lab products found in correlation
4 protocols using fitc annexin v 7 aad kit
1
Annexin-V and 7-AAD Apoptosis Assay
2
Apoptosis Evaluation by Flow Cytometry
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
Apoptosis was evaluated by citofluorimetric analysis of Annexin-V and 7-amino-actinomycin-D (7-AAD)-positive cells using the fluorescein isothiocyanate (FITC)-Annexin-V/7-AAD kit (Beckman Coulter, Milan, Italy). Stained cells were analyzed using the FACSCalibur™ (Becton Dickinson). Positive staining for Annexin-V as well as double staining for Annexin-V and 7-AAD were interpreted as signs of early and late phases of apoptosis respectively [37 (link)].
3
Apoptosis Evaluation by Flow Cytometry
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
Apoptosis was evaluated by cytofluorimetric analysis of Annexin-V and 7-amino-actinomycin-d (7-AAD)-positive cells using the FITC-Annexin-V/7-AAD kit (Beckman Coulter, Milan, Italy). Stained cells were analyzed using the FACSCalibur (Becton Dickinson). Positive staining for Annexin-V as well as double staining for Annexin-V and 7-AAD was interpreted as signs of early and late phases of apoptosis, respectively (Maddalena et al. 2011) (link).
4
Evaluating [18F]FDG Cytotoxicity in Cells
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
To determine [ 18 F]FDG cytotoxicity in labeled cells, 1 × 10 5 cells were stained with 5 mL of annexin V and 5 mL of 7-aminoactinomycin-D (7-AAD) using the fluorescein isothiocyanate (FITC) annexin V/7-AAD kit according to the manufacturer's protocol (Beckman Coulter, Milan, Italy). Positive cells were eva-luated by cytouorimetric analysis using the automated benchtop flow cytometry system FACSCalibur (Becton Dickinson, Franklin Lakes, NJ). Positive staining for annexin V and double staining for annexin V and 7-AAD were interpreted as signs of early and late phases of apoptosis, respectively. 15, (link)16 (link)
About PubCompare
Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.
We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.
However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.
Ready to get started?
Sign up for free.
Registration takes 20 seconds.
Available from any computer
No download required
Revolutionizing how scientists
search and build protocols!