Cd207 pe

Manufactured by BioLegend

Sourced in United States

CD207-PE is a fluorophore-conjugated antibody that targets the CD207 antigen. CD207, also known as Langerin, is a type II transmembrane protein expressed on the surface of Langerhans cells, a type of dendritic cell. The PE (phycoerythrin) fluorophore is used to label the CD207 antibody, enabling the detection and identification of CD207-positive cells through flow cytometry or other fluorescence-based techniques.

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2 protocols using cd207 pe

Multi-parameter Immune Cell Profiling

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Cells were incubated 10 min with the TruStain fcX (clone 93) washed and incubated with the antibodies during 20 minutes followed by two washes with PBS. Monoclonal antibodies specific for mouse molecules were purchased from Biolegend: CD3-FITC (clone 17A2), CD3-APC (clone 17A2), CD3-PerCp/Cy5.5 (clone 17A2), CD8-Brillant Violet 421 (clone 53-6.7), CD45-PE (clone 30-F11), CD45-PErCP(clone 30-F11), CD45.1-PE/Cy7 (clone A20), CD45.1-FITC (clone A20), CD103-APC (clone 2E7), CD103-PerCP (clone 2E7), CD69-APC/Cy7 (clone H1.2F3), CD69-APC (clone H1.2F3), CD44-PerCP (clone IM7), IFN-γ-PE (clone XMG1.2), IFN-γ-APC (clone XMG1.2), TNF-α-APC/Cy7 (clone MP6-XT22), CD11b-FITC (clone M1/70), CD207-PE (clone 4C7), XCR1-APC (clone ZET), XCR1-PerCP-Cy5.5 (clone ZET), CD11c-PE/Cy7 (clone N418), MHCII-APC/Cy7 (clone M5/114.15.2), CD24-PerCP-Cy5.5 (clone M1/69), CD80-APC (clone 16-10A1), CD80-PE/Cy7 (clone 16-10A1), CD86 Brilliant Violet 421 (clone GL-1), CCR7-PE/Cy7 (clone 4B12), IL-2-PE/Cy7 (clone JES6-5H4) IL-12/23-APC (clone C15.6), granzyme B-APC (clone GB11) and viability dye Zombie Aqua (ref 423101). Samples were acquired in a FACSCanto II cytometer (BD Bioscience) and data were analyzed using FlowJo version X.0.7 (Tree Star, Inc.). Gating strategies for all flow cytometry experiments are shown in Supplementary Fig. 4.

Menares E., Gálvez-Cancino F., Cáceres-Morgado P., Ghorani E., López E., Díaz X., Saavedra-Almarza J., Figueroa D.A., Roa E., Quezada S.A, & Lladser A. (2019). Tissue-resident memory CD8+ T cells amplify anti-tumor immunity by triggering antigen spreading through dendritic cells. Nature Communications, 10, 4401.

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Dendritic Cell Immunophenotyping by Flow Cytometry

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The cells were washed and resuspended in PBS. The single-cell suspensions were stained with the following antibodies: CD207-PE (144204, BioLegend, San Diego, CA, USA), FITC-MHCII (I-A-I-E, 107606, BioLegend, San Diego, CA, USA), APC/Cy7-CD11c (117324, BioLegend, San Diego, CA, USA), PE/Cy7-CD86 (25-0862-82, eBioscience, Carlsbad, USA), APC-CD40 (17-0401-82, eBioscience, Carlsbad, USA), PerCP/Cy5.5-7-aminoactinomycin D (7-AAD) (BioLegend, 420404; 0.5 μg/ml, San Diego, CA, USA). Flow cytometry was performed using the NovoCyte flow cytometer and ACEA NovoExpress™ software (ACEA Biosciences, San Diego, CA, USA).

Zhao F., Zhang C., Li G., Zheng H., Gu L., Zhou H., Xiao Y., Wang Z., Yu J., Hu Y., Zeng F., Wang X., Zhao Q., Hu J., Yue C., Zhou P., Huang N., Hao Y., Wu W., Cui K., Li W, & Li J. (2022). A role for whey acidic protein four-disulfide-core 12 (WFDC12) in the pathogenesis and development of psoriasis disease. Frontiers in Immunology, 13, 873720.

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