Pct kit

Arterial blood (5 ml) was extracted from patients before treatment; at 2, 6 and 12 h after treatment; and 12 h after CVVH. The levels of procalcitonin (PCT), tumor necrosis factor-α (TNF-α), interleukin 4 (IL-4), IL-6, IL-8 and IL-10 in serum were measured by ELISA. The PCT kit was purchased from Roche (Mannheim, Germany). TNF-α, IL-4, IL-6, IL-8 and IL-10 kits were all purchased from Beckman Coulter (Brea, CA, USA). All the procedures were performed according to the manufacturer's instructions. The OD values were read by a microplate reader (wavelength, 450 nm), and the corresponding concentrations of PCT, TNF-α, IL-4, IL-6, IL-8 and IL-10 were calculated and recorded.

A total of 5 mL venous blood was extracted from patients before treatment, and at 6, 12, and 24 hours after treatment for 5 days (at 6^th day), respectively, and the day of blood collection was uniform for all patients. The levels of PCT, CRP, IL-17, IL-6, and HMGB1 in serum were detected via enzyme-linked immunosorbent assay (ELSIA). The PCT kit was provided by Roche, German, and the CRP, IL-17, IL-6, and HMGB1 kits were manufactured by Beckman Coulter Inc., USA. In strict accordance with the instructions of relevant kits, the optical density (OD) value was read at a wavelength of 450 nm using a microplate reader, and the levels of PCT, CRP, IL-17, IL-6, and HMGB1 in corresponding samples were calculated. The serum D-dimer level in patients was detected via immunoturbidimetry, and the relevant kits were supplied by Beckman Coulter Inc., USA.

Instruments included a DEM-3 scientific plate washer (Beijing Tuopu Analytical Instrument Co., Ltd., Beijing, China), a Bio-Rad Model 680 microplate reader (Bio-Rad Laboratories, Inc., Hercules, CA, USA), an E601 electrochemistry luminescence analyzer (Roche Diagnostics, Basel, Switzerland), a BN-II specific protein apparatus (Siemens AG, Munich, Germany), and a UniCel DxI 800 immunoassay (Beckman Coulter, Inc., Brea, CA, USA). Reagents included a PCT kit (Roche Diagnostics), IL-6 kit (Beckman Coulter, Inc.), and CRP kit (Siemens AG).