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Individual elisa kits

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Individual ELISA kits are laboratory tools used to detect and measure specific proteins, hormones, or other molecules in a sample. The kits contain all the necessary reagents and materials required to perform a single enzyme-linked immunosorbent assay (ELISA) test.

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ELISAs) (2 citations)

4 protocols using individual elisa kits

1

Cytokine Quantification in Neutrophils

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Cytokine (IL6 and TNFα) determinations were performed in neutrophil-free supernatant using individual ELISA kits (Diaclone, lit for GEN-PROBE) following the manufacturer's instructions for use. The overall intra-assay coefficient of variation was calculated to be 3.3% for TNFα and 4.4% for IL6; the calculated overall interassay coefficient of variation was 9.0% for TNFα and 9.1% for IL6.
Capó X., Martorell M., Sureda A., Tur J.A, & Pons A. (2015). Effects of Docosahexaenoic Supplementation and In Vitro Vitamin C on the Oxidative and Inflammatory Neutrophil Response to Activation. Oxidative Medicine and Cellular Longevity, 2015, 187849.
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2

Cytokine Quantification by ELISA

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Cytokine (TNFα and IL-6) levels were determined in plasma using individual ELISA kits (Diaclone, Besancon Cedex, France) following the manufacturer’s instructions to use. The overall intra-assay coefficient of variation was calculated to be 3.2% for TNFα and 4.4% for IL-6; the calculated overall inter-assay coefficient of variation was 10.9% for TNFα and 9.1% for IL-6.
Monserrat-Mesquida M., Quetglas-Llabrés M., Capó X., Bouzas C., Mateos D., Pons A., Tur J.A, & Sureda A. (2020). Metabolic Syndrome Is Associated with Oxidative Stress and Proinflammatory State. Antioxidants, 9(3), 236.
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3

Plasma Biomarker Measurement Protocol

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IL-6, sCD62L, and sICAM3 plasma levels were determined using individual ELISA kits from Diaclone (Besançon, France). TNFα was determined using the RayBiotech (Norcross, GA, USA) ELISA kit. All procedures were performed following the supplier instructions for use.
MPO activity in plasma was measured by guaiacol oxidation, under identical conditions to those previously described [27 (link)].
Ferrer M.D., Capó X., Martorell M., Busquets-Cortés C., Bouzas C., Carreres S., Mateos D., Sureda A., Tur J.A, & Pons A. (2018). Regular Practice of Moderate Physical Activity by Older Adults Ameliorates Their Anti-Inflammatory Status. Nutrients, 10(11), 1780.
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4

Plasma Cytokine Quantification by ELISA

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Cytokine (TNFα and IL-6) levels were determined in plasma of all participants using individual ELISA kits (Diaclone, Besancon Cedex, France) following the manufacturer’s instructions for use. The intra- and inter-assay coefficients of variation were 3.2% and 10.9% for TNFα and 4.4% and 9.1% for IL-6, respectively.
Monserrat-Mesquida M., Quetglas-Llabrés M., Bouzas C., Capó X., Mateos D., Ugarriza L., Tur J.A, & Sureda A. (2021). Peripheral Blood Mononuclear Cells Oxidative Stress and Plasma Inflammatory Biomarkers in Adults with Normal Weight, Overweight and Obesity. Antioxidants, 10(5), 813.
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