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Anti mouse cd8 53 6

Manufactured by Thermo Fisher Scientific
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The Anti-mouse CD8 (53-6.7) is a lab equipment product used for the detection and analysis of CD8-positive cells in mouse samples. It functions as a cell surface marker, allowing for the identification and quantification of cytotoxic T cells and other CD8-expressing cell types.

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Lab products found in correlation

Anti mouse foxp3 fjk 16s, by Thermo Fisher Scientific (3 mentions) Live dead fixable aqua dead cell stain kit, by Thermo Fisher Scientific (2 mentions) Interleukin 2 (il 2), by Thermo Fisher Scientific (2 mentions) Anti ifn γ, by BioXCell (2 mentions) Anti il 12, by BioXCell (2 mentions) Anti il 4, by BioXCell (2 mentions) Cytofix cytoperm buffer, by Thermo Fisher Scientific (2 mentions) Anti cd28, by Thermo Fisher Scientific (2 mentions) Anti mouse helios 22f6, by BioLegend (2 mentions) Anti mouse ki67 b56, by BD (2 mentions) Anti mouse cd4 rm4 5, by Thermo Fisher Scientific (2 mentions) Anti cd3, by Thermo Fisher Scientific (2 mentions) Facscanto 2 cell analyzer, by BD (2 mentions) Tgf β, by R&D Systems (2 mentions) Facscalibur, by BD (1 mentions) Cd11b m1 70, by Thermo Fisher Scientific (1 mentions) Oct medium, by Electron Microscopy Sciences (1 mentions) Cryostat, by Leica (1 mentions) Er tr7, by Abcam (1 mentions) Anti mouse ifn γ xmg1, by Thermo Fisher Scientific (1 mentions)

Close spelling variants of this product

Anti-CD8 (106 citations) CD8 (53-6.7, (27 citations) Anti-CD8 (53-6.7, (16 citations) Anti-mouse CD8 (6 citations) Pacific blue-anti-mouse CD8 (53-6.7, (2 citations) Anti-mouse CD8 (clone 53–6.7, (2 citations)

5 protocols using anti mouse cd8 53 6

1

Induced Regulatory T Cell Differentiation

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Naïve CD4+ T cells were incubated in Treg cell differentiation condition in the presence of the following: plate-bound anti-CD3 (2 µg/ml, eBioscience), anti-CD28 (3 µg/ml, eBioscience), anti-IL-4 (10 µg/ml, BioXCell), anti-IFN-γ (10 µg/ml, BioXCell), anti-IL-12 (10 µg/ml, BioXcell), IL-2 100 U/ml, Peprotech) and TGF-β (5 ng/ml, R&D Systems). Cells were stained for surface markers with anti-mouse CD4 (RM4–5, eBioscience) and anti-mouse CD8 (53-6.7 eBiosciences). Intracellular staining was performed using Cytofix/Cytoperm buffer (eBioscience) to fix and permeabilize cells. Cells were then washed and stained with following antibodies: anti-mouse Helios (22F6, BioLegend), anti-mouse Ki67 (B56, BD Biosciences) and anti-mouse Foxp3 (FJK-16s, eBioscience). Dead cells were excluded from analysis using LIVE/DEAD Fixable Aqua Dead Cell Stain Kit (Life Technologies). Sample were run on FACSCanto II cell analyzer (BD Biosciences) and analyzed with FlowJo software.
Placek K., Hu G., Cui K., Zhang D., Ding Y., Lee J.E., Jang Y., Wang C., Konkel J.E., Song J., Liu C., Ge K., Chen W, & Zhao K. (2017). MLL4 prepares the enhancer landscape for Foxp3 induction via chromatin looping. Nature immunology, 18(9), 1035-1045.
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2

Multicolor flow cytometry of T-cell subsets

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Biotinylated antibodies against hCD8 (HIT8a) and mouse CD4 (GK1.5) in conjunction with streptavidin–allophycocyanin and phycoerythrin‐conjugated anti‐hCD4 (RPA‐T4) and anti‐mouse CD8 (53‐6.7) antibodies (all from eBioscience, San Diego, CA, USA) were used. Flow cytometry was carried out using a FACSCalibur instrument (BD Biosciences, Franklin Lakes, NJ, USA) and FlowJo software version 7.6.5 (Tree Star, Ashland, OR, USA).
Kasugai Y., Yoshida N., Ohshima K., Matsuo K., Seto M, & Tsuzuki S. (2016). New mouse model of acute adult T‐cell leukemia generated by transplantation of AKT, BCLxL, and HBZ‐transduced T cells. Cancer Science, 107(8), 1072-1078.
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3

Induced Regulatory T Cell Differentiation

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Naïve CD4+ T cells were incubated in Treg cell differentiation condition in the presence of the following: plate-bound anti-CD3 (2 µg/ml, eBioscience), anti-CD28 (3 µg/ml, eBioscience), anti-IL-4 (10 µg/ml, BioXCell), anti-IFN-γ (10 µg/ml, BioXCell), anti-IL-12 (10 µg/ml, BioXcell), IL-2 100 U/ml, Peprotech) and TGF-β (5 ng/ml, R&D Systems). Cells were stained for surface markers with anti-mouse CD4 (RM4–5, eBioscience) and anti-mouse CD8 (53-6.7 eBiosciences). Intracellular staining was performed using Cytofix/Cytoperm buffer (eBioscience) to fix and permeabilize cells. Cells were then washed and stained with following antibodies: anti-mouse Helios (22F6, BioLegend), anti-mouse Ki67 (B56, BD Biosciences) and anti-mouse Foxp3 (FJK-16s, eBioscience). Dead cells were excluded from analysis using LIVE/DEAD Fixable Aqua Dead Cell Stain Kit (Life Technologies). Sample were run on FACSCanto II cell analyzer (BD Biosciences) and analyzed with FlowJo software.
Placek K., Hu G., Cui K., Zhang D., Ding Y., Lee J.E., Jang Y., Wang C., Konkel J.E., Song J., Liu C., Ge K., Chen W, & Zhao K. (2017). MLL4 prepares the enhancer landscape for Foxp3 induction via chromatin looping. Nature immunology, 18(9), 1035-1045.
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4

Quantitative Analysis of Lymphoid Tissues

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Lymph nodes and spleens were removed from PMY-injected or control animals, uninfected or infected with VV as indicated. Organs were cryoprotected in 15% sucrose, embedded in OCT medium (Electron Microscopy Sciences) and frozen in dry-ice cooled isopentane. Fifteen-micron sections were cut on a Leica cryostat (Leica Microsystems). Sections were allowed to air dry and then fixed for 20 min at room temperature (RT) with 3.2% PFA. Sections were washed, blocked with 5% goat or donkey serum, permeabilized with 0.1% Triton-X, then stained with: anti mouse CD8 (53-6.7, eBioscience), CD11b (M1/70, eBioscience), CD38 (RPT-T8, eBioscience), or ERTR-7 (Abcam) along with anti-PMY (clone 2A4) antibody conjugated to Alexa 488. Non-conjugated antibodies were detected with secondary anti-rat antibody conjugated to Alexa 568 (Invitrogen). Slides were counterstained with DAPI (for DNA). Slides were analyzed on an SP5 inverted microscope (Leica Microsystems). For entire lymph node sections, the tile-scan function was used to produce montages covering the whole node. The total fluorescent signal for individual channels (such as the 647 nm wavelength channel for RPM) was quantitated using the measure function of ImageJ software.
Seedhom M.O., Hickman H.D., Wei J., David A, & Yewdell J.W. (2016). Protein Translation Activity: A New Measure of Host Immune Cell Activation. Journal of immunology (Baltimore, Md. : 1950), 197(4), 1498-1506.
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5

Comprehensive Immunophenotyping of Immune Cells

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Fluorochrome-conjugated monoclonal/polyclonal antibodies used in our studies were anti-mouse CD4 (GK1.5, BD Biosciences), anti-mouse CD25 (PC61.5l, eBioscience), anti-mouse Foxp3 (FJK-16s, eBioscience), anti-mouse CD8 (53-6.7, eBioscience), anti-human CD19 (H1B19, eBioscience), anti-human EGFR (AY13, BioLegend), anti-mouse CD45.1 (A20, eBioscience), anti-mouse CTLA-4 (UC10-4B9, eBioscience), anti-mouse neuropilin 1 (eDS304M, eBioscience), anti-mouse Lag-3 (C9B7W, eBioscience), anti-mouse CD11c (N418, eBioscience), anti-mouse IFN-γ (XMG1.2, eBioscience), anti-mouse TNF-α (MP6-XT22, eBioscience), anti-mouse Fas (SA367H8, BioLegend), anti-mouse FasL (MFL3, eBioscience), anti-mouse perforin (eBioMAK-D, eBioscience), anti-mouse GzB (NGZB, eBioscience), anti-mouse GzA (3G8.5, BioLegend), anti-mouse CD107α (1D4B, eBioscience), anti-mouse CD71 (R17217, eBioscience), anti-mouse CPT1a (8F6AE9, Abcam), anti-mouse Glut1 (ER3915, Abcam), and Fixable viability dye eFluor 780 (BD Biosciences). Intracellular staining was performed using the fixation/permeabilization concentrate (catalog 5123-43) and diluent (catalog 5223-56) buffer solutions and IC buffer (catalog 8333-56), according to the eBioscience Foxp3 staining kit. Stained cells were analyzed on an LSR Fortessa flow cytometer (BD Biosciences), and data were analyzed using FlowJo software (Tree Star).
Bolivar-Wagers S., Loschi M.L., Jin S., Thangavelu G., Larson J.H., McDonald-Hyman C.S., Aguilar E.G., Saha A., Koehn B.H., Hefazi M., Osborn M.J., Jensen M.C., Wagner J.E., Pennell C.A, & Blazar B.R. (2022). Murine CAR19 Tregs suppress acute graft-versus-host disease and maintain graft-versus-tumor responses. JCI Insight, 7(17), e160674.
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