3h diazepam

The APC analogs CLR1404, CLR1501 and CLR1502 were provided by Cellectar Biosciences, Inc. (Madison, WI, USA). These APC analogs consist of a cancer-targeting alkylphosphocholine scaffold with an attached cancer imaging, visualization or therapeutic moiety (refs ^{17 , 18 (link), 25 (link), 28 (link)} and Figure 1): iodine isotopes for CLR1404; 4,4-difluoro-4-bora-3a,4a-diaza-s-indacene (i.e. BODIPY, green fluorescence) for CLR1501; and 2-[2-[2-Chloro-3-[2-(1,3-dihydro-1,3,3-trimethyl-2H-indol-2-ylidene)-ethylidene]-1-cyclohexen-1-yl]-ethenyl]-1,3,3-trimethyl-3H-indolium chloride (i.e. IR-775, near infrared fluorescence) for CLR1502. Pharmacological cellular efflux transporter inhibitors were purchased: PSC833 and Ko143 from Tocris Biosciences, and MK571 from Sigma-Aldrich (St. Louis, MO, USA). [¹⁴C]-sucrose and [³H]-diazepam were purchased from American Radiolabeled Chemicals (St Louis, MO, USA).

¹⁴C-mannitol, ¹⁴C-caffeine, ³H-Digoxin and ³H-diazepam were obtained from American Radiolabeled Chemicals, Inc (Saint Louis, MO). Ultima Gold™ liquid scintillation cocktail was purchased from Perkin Elmer (Boston, MA). Valsartan was purchased from Sapphire Bioscience (Redfern, New South Wales, Australia). Digoxin, diazepam and ammonium acetate were obtained from Sigma-Aldrich Pty Ltd (Castle Hill, New South Wales, Australia). HPLC or LC-MS grade acetonitrile and methanol were all purchased from Merck KGaA (Darmstadt, Germany).