Liver tissues were resected (2cm*2cm) and fixed in 10% formalin for 12 hours. Paraffin-embedded sections (4-μm thick) were prepared for immunohistochemical and hematoxylin and eosin (H&E) staining. Sections were incubated with the following primary antibody at 4°C overnight, anti-SIRT3 (Abcam, UK,1:300), anti-SIRT6 (Proteintech.,China,1:100), anti-SIRT7 (Abcam, UK,1:1000). After incubation with labeled the following day using an anti-rabbit universal two-step detection kit (Beijing Zhongshan Jinqiao Biotechnology Co. LTD, China). Finally, the signal was detected by DAB method (Beijing Zhongshan Jinqiao Biotechnology Co. LTD, China). Images were collected using NanoZoomer S60 C13210 series (S60 C13210, Hamamatsu Photonics K.K., Japan).
Anti sirt6
Manufactured by Proteintech
Sourced in United Kingdom
Anti-SIRT6 is an antibody product used for research purposes. It is designed to detect and bind to the SIRT6 protein, which is a member of the sirtuin family of enzymes involved in various cellular processes. The antibody can be used in techniques such as Western blotting, immunoprecipitation, and immunohistochemistry to study the expression and localization of the SIRT6 protein.
Automatically generated - may contain errors
Lab products found in correlation
Anti sirt3, by Abcam (1 mentions)
Acetyl lysine antibody, by ABclonal (1 mentions)
Anti sirt6, by ABclonal (1 mentions)
Anti actb, by Proteintech (1 mentions)
Protease inhibitor cocktail, by Roche (1 mentions)
Anti lc3b, by Proteintech (1 mentions)
Oil red o, by Wuhan Servicebio Technology (1 mentions)
Anti caveolin 1, by Cell Signaling Technology (1 mentions)
Anti h3k9ac, by Cell Signaling Technology (1 mentions)
Anti p62, by Proteintech (1 mentions)
4 protocols using anti sirt6
1
Immunohistochemical Analysis of Liver Sirtuin Proteins
2
SIRT6 Regulation of FXR Acetylation
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
MPHs were harvested and lysed in lysis buffer containing protease inhibitors as previously described. Lysates were immunoprecipitated with anti-SIRT6 (Proteintech)/anti-FXR (Abclonal, Wuhan, China) antibodies or the mouse/rabbit lgG. The total lysates or corresponding immunoprecipitated samples were immunoblotted with anti-SIRT6 and anti-FXR antibodies.
For acetylation assays, Ad-Gfp or Ad-SIRT6 infected MPHs were exposed to APAP (10 mM) treatment for 24 hours. Cells were lysed in lysis buffer containing protease and acetylase inhibitors (Beyotime Biotechnology, Beijing, China). Anti-FXR antibody was used to immunoprecipitate endogenous FXR in protein samples, and acetylated FXR was detected with an acetyl-Lysine antibody (Abclonal, Wuhan, China) using Western blotting.
For acetylation assays, Ad-Gfp or Ad-SIRT6 infected MPHs were exposed to APAP (10 mM) treatment for 24 hours. Cells were lysed in lysis buffer containing protease and acetylase inhibitors (Beyotime Biotechnology, Beijing, China). Anti-FXR antibody was used to immunoprecipitate endogenous FXR in protein samples, and acetylated FXR was detected with an acetyl-Lysine antibody (Abclonal, Wuhan, China) using Western blotting.
3
Protein Extraction and Western Blot Analysis
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
Total protein was extracted from cells using a cell extraction buffer [50 mM Tris-HCl (pH 8.0), 4 M urea, and 1% Triton X-100] containing a protease inhibitor cocktail (Roche Diagnostics, catalogue number 04693132001). The samples were subsequently resolved using SDS-PAGE and analyzed by western blotting. The following antibodies were used: anti-H3 (CTS, #4499, 1:1,000), anti-γH2AX (CTS, #9718, 1:1,000), anti-H3K27cr (RevMab Biosciences, 31-1287-00, 1:800), anti-SIRT6 (Proteintech, #13572-1-AP, 1:1,000), anti-ACTB (Proteintech, #20536-1-AP, 1:5,000), anti-rabbit IgG (KPL, 074-1506, 1:5,000), and anti-mouse IgG (KPL, 074-1806, 1:5,000). The intensity of image was measured by Image J2 software (https://imagej.net/Fiji/Downloads ).
4
Quantifying Stem Cell Senescence in Umbilical Cord
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
Human umbilical cord tissues were fixed with 4% paraformaldehyde, dehydrated overnight in 30% sucrose solution at 4 • C, and stained with Oil Red O (Servicebio Technology CO., Ltd., Wuhan, China) for 30 min. The Oil Red O-positive areas and the staining intensity were quantified using Image J software. The tissue sections were incubated with anti-Sirt6, anti-LC3B, anti-P62 (1:50; Proteintech, Wuhan, China), anti-Caveolin-1, anti-H3K9ac (1:100; Cell Signaling Technology, Inc., Beverly, MA, USA) and anti-H3K56ac (1:100; PTM BIO Technology Co., Ltd., Hangzhou, China) antibodies, followed by secondary antibodies (Absin, China). The positively stained regions were observed under a microscope (Olympus, Tokyo, Japan).
About PubCompare
Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.
We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.
However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.
Ready to get started?
Sign up for free.
Registration takes 20 seconds.
Available from any computer
No download required
Revolutionizing how scientists
search and build protocols!