In this study, all the chemicals, standards, and reagents were purchased from Sigma and Merck and were of analytical grade. Specifically, Diclofenac sodium, Metformin, Baker’s yeast, PBS-solution, and EDTA were purchased from Sigma. However, dichloromethane, methanol, chloroform, ethyl acetate, n-Hexane, butanol, and silica gel column were procured from Merck.
Baker s yeast
Manufactured by Merck Group
Baker's yeast is a common laboratory equipment used in various experimental and research applications. It is a single-celled fungus that is widely used for its ability to ferment carbohydrates, producing carbon dioxide and ethanol as byproducts. Baker's yeast is a versatile tool that can be utilized in a range of laboratory settings to study cellular processes, enzyme activities, and metabolic pathways.
Automatically generated - may contain errors
Lab products found in correlation
Diclofenac sodium, by Merck Group (1 mentions)
Silica gel column, by Merck Group (1 mentions)
Pbs solution, by Merck Group (1 mentions)
Ethylene diamine tetraacetic acid (edta), by Merck Group (1 mentions)
Metformin, by Merck Group (1 mentions)
Methanol, by Merck Group (1 mentions)
Chloroform, by Merck Group (1 mentions)
N hexane, by Merck Group (1 mentions)
Dichloromethane, by Merck Group (1 mentions)
Fla3000 phosphorimager, by Fujifilm (1 mentions)
Genescreen plus, by PerkinElmer (1 mentions)
3 protocols using baker s yeast
1
Analytical Reagents Procurement Protocol
2
Northern Blot Analysis of Gene Expression
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Total RNA was purified as described [102 (link)]. For blotting, 5 and 10 μg of total RNA were separated in 1% denaturing agarose gel, transferred to nylon membrane (GeneScreen Plus; Perkin-Elmer), UV cross-linked, baked and hybridized at 42°C overnight with random primed 32 P-DNA fragments of genes of interest. Hybridization solution was 5X Denhardt’s, 5X SSC, 50% formamide, 0.2% SDS, 5 mM EDTA, 100 μg/ml total yeast RNA (Baker’s yeast, Sigma). Quantitation was by PhosphorImager FLA-3000 (Fuji Film). For sequential probings, signal was stripped, monitored for loss of 32 P before next probing.
3
Enzymatic Starch Conversion Process
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Soluble starch (Kupavnareaktiv, Russia), glucose and α-asparagine (Dia-M, Russia), baker’s yeast (Saff-Moment, France), corn extract (Sigma-Aldrich, United States), malt extract (Fluka, United States), L‑lysine monohydrocloride (PanReac, United States), a sorbent of XAD type (Sigma-Aldrich), Aspergillus oryzae α-amylase (Serva, Germany), and the tacrolimus reference preparation for HPLC (Zhejiang Hisun Pharmaceutical Co. Ltd., China) were used in this work. The rest of the reagents and solvents were of chemically pure or pure for analysis grades from Russian manufacturers.
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