Anti mouse or anti rabbit westernbreeze chromogenic immunodetection system kits

Treated cells or pellets of isolated EV were lysed with 100 μL lysis buffer and protein concentrations were determined using the Pierce™ Rapid Gold BCA Protein-Assay-Kit (ThermoFisher). Equivalent amounts of protein were subjected to 10% Mini-PROTEAN^® TGX™ Precast Protein Gels (Bio-Rad, Feldkirchen, Germany). Gels were blotted using a Mini Trans-Blot^® Cell (Bio-Rad) onto Amersham™ nitrocellulose membranes (Merck). All primary antibodies used are given in Table 1. Bound antibodies on the membranes were visualized using anti-mouse or anti-rabbit WesternBreeze^® Chromogenic Immunodetection System Kits (ThermoFisher). Band densities were quantified using Image J software (version 1.50i; National Institutes of Health, Bethesda, MA, USA).

Treated cells or pellets of isolated EVs were lysed with a 100 μL lysis buffer and protein concentrations were determined using the Pierce™ Rapid Gold BCA Protein-Assay-Kit (Thermo Fisher). Equivalent amounts (10–50 μg) of protein were subjected to 10% Mini-PROTEAN^® TGX™ Precast Protein Gels (Bio-Rad, Feldkirchen, Germany). Blotting of gels was performed using a Mini Trans-Blot^® Cell (Bio-Rad). All primary antibodies used are given in Table 2. Bound antibodies on the membranes were visualized using anti-mouse or anti-rabbit WesternBreeze^® Chromogenic Immunodetection System Kits (Thermo Fisher). Band densities were quantified using Image J software (version 1.50i; National Institutes of Health, Bethesda, MA, USA).