Human primary keratinocytes were seeded onto plastic inert filters (ThinCerts, Greiner Bio-one, Breda, The Netherlands) in CnT-PR medium (CELLnTEC, Bern, Switzerland). After 48h, cells were switched to CnT-PR-3D barrier (CELLnTEC) for 24h, and then cultured at the air-liquid interface for 10 days. Human skin equivalents using de-epidermised dermis were generated as described previously (van den Bogaard et al., 2012 (link)). Skin equivalents were treated, as indicated, with 500 nM GNF351 or 5 μM CH223191.
Cnt pr 3d barrier
Manufactured by CELLnTEC
The CnT-PR-3D barrier is a laboratory equipment designed to measure the transepithelial electrical resistance (TEER) of cell cultures grown on permeable supports. The device provides a non-invasive method to evaluate the integrity of cellular barriers, which is an important parameter in various research applications.
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Lab products found in correlation
3 protocols using cnt pr 3d barrier
1
Generating Skin Equivalents for Barrier Analysis
2
3D Culture of Keratinocytes with Inhibitors
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Mouse (5 × 105 cells) and human (2 × 105 cells) keratinocytes were seeded onto 12-mm diameter inserts (Millipore, cat. #PIHP01250) and cultured in CnT-Prime medium. After two days, the medium was replaced with CnT-PR 3D-Barrier (CellnTec, cat. #CnT-PR-3D); 16 h later, the airlift was performed according to the manufacturer’s instructions. 3D cultures were maintained in CnT-PR 3D-Barrier for 12 days and ultimately fixed in 4% paraformaldehyde to be processed for immunohistochemical analysis. During the final seven days of the culture, cells were treated with the appropriate vehicles and inhibitors, including 1A116 (500 nM), FRAX597 (5 nM), Y-27632 (1 μM), 10,058-F4 (500 nM), verteporfin (100 nM), and CX-5461 (100 nM). Concentration of inhibitors were chosen based on their minimal effect on control cells as indicated above.
3
Generating Skin Equivalents for Barrier Analysis
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
Human primary keratinocytes were seeded onto plastic inert filters (ThinCerts, Greiner Bio-one, Breda, The Netherlands) in CnT-PR medium (CELLnTEC, Bern, Switzerland). After 48h, cells were switched to CnT-PR-3D barrier (CELLnTEC) for 24h, and then cultured at the air-liquid interface for 10 days. Human skin equivalents using de-epidermised dermis were generated as described previously (van den Bogaard et al., 2012 (link)). Skin equivalents were treated, as indicated, with 500 nM GNF351 or 5 μM CH223191.
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