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Cd38 alexa fluor 700

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CD38 Alexa Fluor 700 is a fluorochrome-conjugated antibody that binds to the CD38 cell surface antigen. It is designed for use in flow cytometry and other fluorescence-based applications to detect and analyze cells expressing CD38.

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Lab products found in correlation

Cd34 pe, by Thermo Fisher Scientific (2 mentions) Facsdiva, by BD (2 mentions) Cd45ra brilliant violet 570, by BioLegend (2 mentions) Cd49f percp cy5, by BioLegend (1 mentions) Isotype control, by BD (1 mentions) Cd10 pe cy7, by BioLegend (1 mentions) Goat anti rabbit fitc, by Thermo Fisher Scientific (1 mentions) Sa apc cy7, by BioLegend (1 mentions) Fixation buffer, by BD (1 mentions) Appropriate isotype control, by BioLegend (1 mentions) Cd49f bv421, by BioLegend (1 mentions) Cd138 pe, by BioLegend (1 mentions) Igd apc cy7, by BioLegend (1 mentions) Cd27 fitc, by BioLegend (1 mentions) Facsaria 2, by BD (1 mentions) Cellquest, by BD (1 mentions) Cd45 percp, by BD (1 mentions) Cd19 apc, by BD (1 mentions) Anti human cd3 fitc, by BD (1 mentions) Cd16 cd56 pe, by BD (1 mentions)

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Alexa Fluor 700 (29 citations)

4 protocols using cd38 alexa fluor 700

1

Comprehensive Hematopoietic Progenitor Profiling

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Mononuclear cell surface markers were stained with the following fluorochromes: human hematopoietic lineage markers (CD2, CD3, CD14, CD16, CD19, CD56, CD235a) APC (eBioscience), CD34 PE, CD38 Alexa Fluor 700, CD7 PE-Cy5, CD10 PE-Cy7, CD49f PerCP-Cy5.5, CD45RA Brilliant Violet 570, CD135-Biotin, CD127 (IL7Rα) PE-Cy7, SA-APC-Cy7 (BioLegend). Following surface marker staining, cells were fixed with 2% paraformaldehyde, permeabilized with 0.1% Tween-20 and stained with goat anti-human ARID3a antibody (18 (link)), followed by rabbit anti-goat FITC (Invitrogen). Isotype controls (BD Biosciences, eBiosciences, BioLegend) were used for gating hematopoietic progenitor subsets as described (22 (link), 24 (link)) . Doublet exclusion was used to ensure analyses of single cells prior to forward/side scatter gating. Data were collected using an LSRII (BD Biogenics) and FACSDiva (BD Biosciences) software version 4.1, and were analyzed using FlowJo (Tree Star) software version 10.
Ratliff M.L., Ward J.M., Merrill J.T., James J.A, & Webb C.F. (2014). Differential Expression of the Transcription Factor ARID3a in Lupus Patient Hematopoietic Progenitor Cells. Journal of immunology (Baltimore, Md. : 1950), 194(3), 940-949.
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2

Identification of Hematopoietic Cell Markers

Cited 2 times
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Cell surface markers were identified with the following fluorochromes: human hematopoietic lineage markers (CD2, CD3, CD14, CD16, CD19, CD56, CD235a) conjugated to APC (eBioscience), CD34 PE, CD38 Alexa Fluor 700, CD49f BV421, and CD45RA Brilliant Violet 570 (all from BioLegend). Appropriate isotype controls (BioLegend) were used for gating HSCs as described [29 (link), 30 (link)]. In some cases, following surface marker staining, cells were fixed with fixation buffer (BD Biosciences), permeabilized with Transcription Factor Fixation/Permeabilization Buffer kit (eBioscience) and stained with goat anti-human ARID3a, followed by rabbit anti-goat FITC secondary antibody (Invitrogen) to identify ARID3a-expressing cells and/or with H2A.X phopspho (Ser139) APC-Fire750 (Biologend). Human anti-ARID3a peptide specific antibodies were generated and isolated over a peptide-specific column as described [55 (link)]. Cells were pretreated with anti-human CD32 antibody to block Fc receptor binding prior to surface staining to exclude non-specific binding. Doublet exclusion was used to ensure analyses of single cells prior to forward/side scatter gating. Data were collected using an LSRII (BD Biogenics) and FACSDiva (BD Biosciences) software version 4.1 or Stratedigm S1200Ex and CellCapTure acquisition software and were analyzed using FlowJo (Tree Star) software version 10.
Ratliff M.L., Garton J., James J.A, & Webb C.F. (2020). ARID3a expression in human hematopoietic stem cells is associated with distinct gene patterns in aged individuals. Immunity & Ageing : I & A, 17, 24.
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3

PBMC Isolation and Immunophenotyping

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Peripheral blood mononuclear cells (PBMCs) were isolated from blood samples with an EDTA tube by density gradient centrifugation using Ficoll–Paque, then resuspended in a freezing solution and stored in liquid nitrogen. Frozen PBMCs were thawed and washed, then stained with fluorescently labeled monoclonal antibodies [anti-human CD3-FITC, CD16/CD56-PE, CD45-PerCP, CD19-APC (Beckton Dickens (BD), MultitestTM), CD19-APC, CD27-FITC, CD24-PerCP, CD38-Alexa Fluor 700, IgD-APC/Cy7, and CD138-PE (Biolegend)] and, then six color immunofluorescence staining was utilized (BD FACS Aria II, Becton Dickinson, Franklin Lakes, NJ). Data were analyzed using Cell Quest (BD) and FlowJo7.6.5 software.
Ozdag Acarli A.N., Tuzun E., Sanli E., Koral G., Akbayir E., Cakar A., Sirin N.G., Soysal A., Aysal F., Durmus H., Parman Y, & Yilmaz V. (2023). Disease activity in chronic inflammatory demyelinating polyneuropathy: association between circulating B-cell subsets, cytokine levels, and clinical outcomes. Clinical and Experimental Immunology, 215(1), 65-78.
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4

Murine Germinal Center Characterization

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Wild-type mice (C57BL/6) were provided by Harlan, Israel. All experiments with mice were approved by the Weizmann Institute IACUC committee. Mice were immunized by injection of OVA (25 μl) into the hind footpad, coupled to the hapten NP-OVA precipitated in alum (Imject Alum, Thermo Scientific). Single-cell suspensions were obtained by forcing popliteal lymph node through a 70-μm mesh into ice-cold FACS buffer (EDTA 1 mM and 2% serum in PBS). Cells were incubated with 2 μg ml−1 anti-16/32 (clone 93) for blockage of fragment crystallizable receptors, for 5–10 min. Cell suspensions were washed and incubated with fluorescently labeled antibodies (B220 V500, FAS FITC, CD38 Alexa fluor 700; Biolegend) for 20–40 min. Germinal center cells were gated as live/single, B220+ CD38Lo FASHi. Cell suspensions were analyzed using a Cytoflex (Beckman) flow cytometer.
Dubiella C., Pinch B.J., Koikawa K., Zaidman D., Poon E., Manz T.D., Nabet B., He S., Resnick E., Rogel A., Langer E.M., Daniel C.J., Seo H.S., Chen Y., Adelmant G., Sharifzadeh S., Ficarro S.B., Jamin Y., da Costa B.M., Zimmerman M.W., Lian X., Kibe S., Kozono S., Doctor Z.M., Browne C.M., Yang A., Stoler-Barak L., Shah R.B., Vangos N.E., Geffken E.A., Oren R., Koide E., Sidi S., Shulman Z., Wang C., Marto J.A., Dhe-Paganon S., Look T., Zhou X.Z., Lu K.P., Sears R.C., Chesler L., Gray N.S, & London N. (2021). Sulfopin is a covalent inhibitor of Pin1 that blocks Myc-driven tumors in vivo. Nature chemical biology, 17(9), 954-963.
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