NCM460-NTR1 cells were generated from human colonic epithelial NCM460 cells (INCELL, San Antonio, Texas, USA) transduced with lentivirus particles as previously described10 (link) and maintained in M3:D culture media (INCELL) supplemented with 10% fetal bovine serum (FBS, Life Technologies, Grand Island, New York, USA). Human embryonic kidney fibroblasts, HEK293, and colonic cancer HCT-116 cells were maintained in Eagle's Minimum Essential Medium (MEM, Life Technologies) and McCoy5a (ATCC, Manassas, Virginia, USA), respectively, and supplemented with 10% FBS. Lipofectamine 2000, lipofectamine RNAimax and OptiMEM were from Life Technologies. NT was from Bachem Americas (Torrance, California, USA). TNBS and SR48962 were from Sigma Aldrich (St Louis, Missouri, USA), and DSS was from MP Biomedicals (Santa Ana, California, USA). Goat anti-AFTPH (sc-167055), mouse antivillin and rabbit anti-β tubulin were from Santa Cruz Biotechnology (Santa Cruz, California, USA).
M3 d culture media
Manufactured by INCELL
Sourced in United States
M3:D culture media is a specialized cell culture medium designed for the maintenance and growth of various cell types. It provides a balanced formulation of nutrients, growth factors, and other essential components to support cell viability and proliferation.
Automatically generated - may contain errors
Lab products found in correlation
Rabbit anti β tubulin, by Santa Cruz Biotechnology (2 mentions)
Lipofectamine rnaimax, by Thermo Fisher Scientific (2 mentions)
Lipofectamine 2000, by Thermo Fisher Scientific (2 mentions)
Dextran sulfate sodium (dss), by MP Biomedicals (2 mentions)
Minimum essential medium (mem), by Thermo Fisher Scientific (2 mentions)
Opti mem, by Thermo Fisher Scientific (2 mentions)
Mccoy5a, by American Type Culture Collection (2 mentions)
Sr48962, by Merck Group (2 mentions)
Ncm460, by INCELL (2 mentions)
Neurotensin, by Bachem (1 mentions)
2 protocols using m3 d culture media
1
Generation and Maintenance of NCM460-NTR1 Cells
2
Modeling Colonic Epithelial Cell Lines
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